Probiotics in Food Systems: Significance and Emerging Strategies Towards Improved Viability and Delivery of Enhanced Beneficial Value.

Preserving the efficacy of probiotic bacteria exhibits paramount challenges that need to be addressed during the development of functional food products. Several factors have been claimed to be responsible for reducing the viability of probiotics including matrix acidity, level of oxygen in products, presence of other lactic acid bacteria, and sensitivity to metabolites produced by other competing bacteria. Several approaches are undertaken to improve and sustain microbial cell viability, like strain selection, immobilization technologies, synbiotics development etc. Among them, cell immobilization in various carriers, including composite carrier matrix systems has recently attracted interest targeting to protect probiotics from different types of environmental stress (e.g., pH and heat treatments). Likewise, to successfully deliver the probiotics in the large intestine, cells must survive food processing and storage, and withstand the stress conditions encountered in the upper gastrointestinal tract. Hence, the appropriate selection of probiotics and their effective delivery remains a technological challenge with special focus on sustaining the viability of the probiotic culture in the formulated product. Development of synbiotic combinations exhibits another approach of functional food to stimulate the growth of probiotics. The aim of the current review is to summarize the strategies and the novel techniques adopted to enhance the viability of probiotics.

1. Introduction

Agricultural and food industry are constantly evolving entailing innovation phenomena that generate constant research and emerging technologies. The change of consumers’ preferences, needs and acceptances is a dynamic process, hence the maintenance of food quality via technology innovation is evident [1]. The cultural heritage of consumers, habits, and even sustainability factors can similarly affect technology innovations applied in the food industry [2,3].

Nowadays, consumers are becoming more health conscious and concerned about the beneficial value of food, thus directing manufacturers to emphasize on the promotion of functional foods. Therefore, the key for successful marketing and acceptance of novel foods depends not only on the concept of food quality throughout the chain but also to added value food functionalities [4]. These novel functional food products are either natural or processed foods that have been fortified with active compounds of known biological activity. These compounds when administered in defined quantitative and qualitative amounts, will provide clinically proven health benefits apart from those delivered by fundamental nutrients [5]. The development of probiotic food formulations is a key research area for the future functional food market. Economic forecasts expect an increase from 3.3 to 7 US$ billion for the global market of probiotic dietary supplements from 2015 to 2025 [6].

Probiotics are defined as “live microorganisms which when administered in adequate amounts confer a health benefit on the host” [7]. The health benefit of the hosts targets primarily on the modulation of gut microbiota [8,9]. Human gut microbiota includes the indigenous intestinal microflora that participates in diversified functions that improve host health [10]. Guidelines and information from the Food and Agriculture Organization of the United Nations (FAO)/World Health Organization (WHO) demonstrate the necessity for the probiotic strains to remain intact through the upper intestinal tract to ensure health promoting effects upon entering their site of action, regardless the delivery mode applied. For instance, to assure that, it has been stated that the so called “minimum therapeutic” level of viable probiotic microorganisms should be at least 106 CFU/g of viable cells throughout the product shelf-life [11]. One of the most significant features of probiotics is the production of substances like antibiotics, anticarcinogens, or other compounds with health promoting or pharmaceutical properties [12,13,14]. A recently emerging concept attracting substantial research and industry interest with respect to probiotics is their role in the gut-brain axis. Recent evidence and ongoing studies suggest that intestinal microbiota has a bi-directional effect on mood disorders. Research is focusing on interactions among probiotic modulation of gastrointestinal tract (GIT) and neurological or neuropsychiatric disorders via the enteric and central nervous system [15,16,17,18]. The central nervous systems, the autonomic nervous system (sympathetic and parasympathetic arms), the enteric nervous system, the hypothalamic pituitary adrenal axis and the gut microbiota, form the brain gut microbiota axis [19]. Sharkar et al. [20] used the term psychobiotics to define probiotic bacteria than upon ingestion convey mental health benefits, also including prebiotics that will stimulate the activity of beneficial microflora [20].

Based on the amount of ingested food along with the effect of storage on probiotic viability, it was suggested that a daily intake of 108–109 cfu/g probiotic bacteria could survive the upper ingestion to exert their positive physiological functions in the human body [21]. Karimi et al. [22] have stated that ~100 g/day of probiotic products should be consumed in order to deliver about 109 viable cells into the intestine on a regular daily basis [22]. Regular probiotics consumption was evidenced by dose-response studies, indicating the transient ability of L. rhamnosus GG to colonize GIT [23]. For instance, fifteen days after terminating the intervention in adults, Lactobacillus GG was recovered from stool samples of only 27% of the volunteers [23].

The inclusion of probiotics into a food matrix poses several technological challenges that need to be addressed [24]. These bacteria encounter a variety of stress factors, including temperature, acid and bile, increased concentration of certain ions or nutrient depletion, exposure to osmotic and oxidative stress in product matrices along with passage through the gastrointestinal transit that may detrimentally affect their viability and their functionality. Probiotics need either to adapt to such a dynamic environment or to be protected in order to survive and become available in adequate quantities and deliver their health benefits. Moreover, the addition of probiotics may alter the taste and aroma of the final food product due to production of different metabolites such as organic acids during fermentation and extended storage. Thus, it is essential, that the incorporation of a probiotic culture does not affect adversely the sensory and other quality characteristics of the product [25,26].

2. Probiotics and Delivery Systems

Food products supplemented with probiotics can carry a single or many different bacterial strains. Specifically, in fermented food products, probiotics can be added as starter cultures while other bacterial or yeast strains co-exist. However, the crucial point is for probiotics to be maintained in high counts during products shelf life. Although several bacteria strains and yeasts have been so far characterized as potentially probiotic, Lactobacillus and Bifidobacterium species constitute the main representatives thus being the ones most commonly studied. Both genera have a long history of safe use and have been characterized as “generally recognized as safe” (GRAS), being also dominant inhabitants in the microbiota of the human intestine [27]. Other species belonging to the genera of Lactococcus, Enterococcus, Propionibacteria, and Saccharomyces (e.g., S. cerevisiae and S. boulardii) are also included in the list of probiotics mainly because of their known health-promoting effects [28,29,30,31]. Bifidobacteria are anaerobic bacteria and most strains do not grow under atmospheric conditions of less than 90% air and 10% CO2. The optimal growth temperature is around 37 °C at a pH value of 6.5–7 [32]. For lactobacilli, the optimal growth temperature ranges from 30 to 40 °C, at a pH of 6.5. Also, some thermophilic strains grow well and present highly activated metabolism at temperatures around 45 °C [33,34,35]. Recent research is focusing on the characterization of novel strains with beneficial impact that constitute natural inhabitants in the gut. For instance, the probiotic and beneficial potential of Akkermansia municiphila with respect to immuno-modulation and gut modulation has been investigated [36]. Faecalibacterium prausnitzii strains, belonging to Firmicutes phyla, were recently isolated and evaluated for their probiotic potential [37]. The specific bacterium has been reported to decrease in patients with intestinal disorders, including Crohn’s disease, whereby it was proposed that application of F. prausnitzii as a probiotic could modulate dysbiosis phenomena in the gut [38].

Several systems have been developed for the delivery of probiotics to the GIT such as pharmaceutical formulations and food-based products. Pharmaceutical preparations in particular are considered more effective compared to commercial food-based carrier systems although their preference mainly depends on consumers’ perception [4]. Examples of pharmaceuticals for the delivery of probiotics currently include, capsules, liquids, powder beads, and tablets [39]. Strain selection and differentiation is considered a crucial step in both pharmaceutical and food systems (Figure 1). Likewise, an issue that needs evaluation is the fact that the preparation methods for the delivery matrix may affect cell viability and effective target release in the GIT.

Figure 1

Criteria for the selection of probiotic strains.

Food-based probiotic products account for a large number of probiotic formulations and can be divided in two distinct categories: Dairy products e.g., cheeses, yogurts, ice cream, milk, acidified milks and creams and non-dairy products, e.g., meats and meat products, bread or other fiber snacks, chocolates, fruit juices and other fruit preparations [40,41,42,43,44]. The vast availability of food products makes them a good and potentially effective carrier system for the delivery of probiotics. Nevertheless, their ability to deliver viable cells to the human intestine may differ considerably, depending largely on the physicochemical properties of each composite food matrix.

3. Significance of Cell Viability

Bacterial viability refers to the ability of a cell to grow and subsequently generate a colony of cells under defined environmental conditions [24]. Viability is generally considered as a prerequisite for the functionality of probiotics as it relates with consumers health-promoting properties, thus it constitutes an industrial challenge. Several studies have reported the significance of viable cells with respect to functional properties on probiotic characteristics. Antimicrobial compounds and short chain fatty acids are indicative metabolites produced from viable colonies [45]. Galdeano et al. conducted a study in mice on the effect of viable and non-viable lactobacilli and their persistence in gut and mucosal immune stimulation [46]. The authors demonstrated that the viability of bacteria was necessary to stimulate the gut immune system. In another clinical study, Pelletier et al. suggested that viable cells are more effective in lactose digestion compared to non-viable probiotic cells [47]. On the other hand, certain reports have reported functionalities associated with both alive and non-viable probiotics [48,49].

As a result, scientific research has recently focused on many different innovative techniques targeting to enhance the viability of probiotic cells during products’ shelf-life [50]. Many of these methods have been proved to be quite successful, nevertheless enhanced shelf-life of probiotics does not necessarily provide the appropriate robustness of a culture when exposed to the challenging conditions of the GIT [39]. Each probiotic strain may exhibit a different response on various stress factors of the digestive tract. Cell membrane may be affected after consumption, by several factors such as the gastric acid, bile salts, various digestive enzymes, the food matrix or even the host’s microbiota [51]. Likewise, probiotic strains can be detected within the gastrointestinal tract as viable, dormant, active or dead depending on the environmental conditions and the capacity of the strain to survive [51,52]. On the other hand, certain studies have demonstrated that viability is not mandatory for all probiotic effects as not all cell mechanisms are directly related to viability since even the dead cells proved to provide beneficial effects to the consumer [53]. Research should focus more on the interactions occurring in the gut after the consumption of probiotics considering the indigenous gut microbial diversity of each human [54]. Hence, the main challenge would be to construct a model that could provide a more personalized diet based on ingredients that can reassure consumers optimal health [55]. Implementation of metabolomics as part of transcriptomics and proteomics could elucidate the understanding of interactions between the metabolic pathways of gut microbiota and the host along with the effect of age, gender, lifestyle, diet to allow gut microbiota modulation. For instance, genome-scale metabolic modeling could elucidate host-microbiome-diet interactions to study the microbial gut metabolism in physiological and dysbiosis states [56].

4. Factors Affecting Viability of Probiotics

Many factors have been identified to influence the viability of probiotics in food products during processing and storage (Figure 2). These factors include intrinsic parameters of the product like pH, titratable acidity, oxygen, water activity, presence of salt, sugar and other compounds (hydrogen peroxide, bacteriocins, artificial flavoring and coloring agents etc.), processing parameters including fermentation conditions (incubation temperature, heat treatment, cooling and storage conditions of the product, packaging materials, scale of production), and finally microbiological parameters (strain of probiotics employed, rate and proportion of inoculation) [39,52].

Figure 2

Factors affecting viability of probiotics in food products (during processing and storage), as well as in the gastrointestinal tract (GIT).

4.1. Chemical Factors

Food components such as additives (sugars, salt, antimicrobials, aroma compounds, or even bacteriocins) can positively or negatively affect probiotic cells’ viability. Antimicrobial compounds and bacteriocins can significantly challenge the viability of probiotics in a food matrix, especially during storage, whereas prebiotics are known to have a positive effect on the viability of probiotics [57,58].

Oxygen levels and redox potential are also among the important factors affecting the viability of probiotic cultures, especially during storage, and primarily refer to anaerobic bacteria such as bifidobacteria [59]. The effect of oxygen on cell viability largely differentiates within the genera encountered in gut microbiota. For instance, lactobacilli are more tolerant to oxygen than bifidobacteria, to the point where oxygen levels are rarely an important issue in maintaining the survival of the former [60]. Therefore, oxygen concentration and oxygen permeability of the packaging should be maintained at low levels to effectively control losses in probiotic viability. Several methods have been proposed to reduce the oxygen content in packaged probiotic foods, e.g., vacuum packaging, addition of antioxidants or oxygen scavengers like ascorbic acid [61]. The sensitivity of oxygen, including bifidobacteria that are obligate anaerobes, limits their survival and use in industrial applications [62,63]. Several methods have been used to decrease oxygen levels during fermentation of products, like fermentation under vacuum-anaerobic conditions, addition of oxygen scavengers or genetic manipulation of bifidobacteria [64].

The water activity is another factor that may affect probiotic survival. Cell viability is particularly affected when a dry food matrix has an elevated water activity (a > 0.25) [65]. Titratable acidity and low pH values can also affect the viability of probiotics during storage. Usually, fruit juices with low pH and a high organic acid content impose a significant stress challenge to probiotics. Champagne, et al. [66] inoculated Lactobacillus rhamnosus R0011 in an apple-pear-raspberry juice blend, at 4.5 × 109 CFU/250 mL portion, and the viability was monitored during storage under conditions simulating consumer handling [66]. When the juices remained closed in the PET (polyethylene terephthalate) bottles, the viable population gradually dropped by 75% over 5 weeks of storage at 7 °C, while in opened bottles the reduction was only between 20% and 40%. Moreover, Ding and Shah [67] investigated the survival of eight different strains of free and microencapsulated probiotic bacteria in orange and apple juices during six weeks storage [67]. They reported that encapsulated probiotic bacteria survived in fruit juices throughout the entire storage period, whereas free probiotic bacteria showed a reduction in viability within five weeks. In general, fruit juices containing encapsulated probiotic bacteria were more stable than those containing free probiotic microorganisms. More recently, the survival of free Bifidobacterium longum NCIMB 8809 cells was studied during refrigerated storage for 6 weeks in model solutions [68]. Among all factors affecting cell viability, the most important ones that negatively changed cells viability were pH (3.2–4.0), citric acid (2–15 g/L), protein (0–10 g/L), and dietary fiber (0–8 g/L), with the pH and citric acid being the most significant. The highest cell survival (less than 0.4 log decrease) after 6 weeks of storage at 4 °C was observed in orange and pineapple, both of which had a pH value of about 3.8. Although the pH value of grapefruit and blackcurrant were similar (pH ∼ 3.2), there was a 0.2 log reduction difference deriving probably due to the high amount of citric acid of grapefruit (15.3 g/L). The large decrease in cell viability (∼8 logs) noted in pomegranate and strawberry juices was attributed to the high levels of phenolic compounds and the very low pH of these juices (pH ≤ 3) [68]. Also, large differences in acid resistance of free lactobacilli and bifidobacteria cells have been observed when added to orange, pineapple and cranberry juices and further stored at 4 °C [69]. Overall, encapsulation techniques are well documented to convey increased tolerance of probiotics in low pH and high water activity conditions, such as those encountered in fruit juices.

4.2. Biological Factors

Many biological factors such as strain type, antagonism with starter cultures, product natural microflora, produced enzymes, post-acidification and occurrence of various pathogenic or spoilage microorganisms are known to influence the viability of probiotics [70,71]. A desirable criterion for the selection of probiotic is to present functional, technological and safety properties, without providing negative features. The selected probiotic strain may show antagonistic effects against various microorganisms resulting in losses of cell viability. In many cases, the added probiotic culture may be affected by the starter culture used for food fermentation. On the other hand, the appropriate probiotic strain can demonstrate several antagonistic mechanisms including competition for nutrients, coaggregation with pathogens, and immune system stimulation [71]. A special reference needs to be addressed regarding the manufacture of fermented probiotic meat products as they are more complicated than other probiotics-containing products. The reasons associate with raw material characteristics [72] such as high salt content, low pH and water activity due to the acidification and drying processes. Fermented fish products have been also studied as a method of probiotic delivery [73]. In general, cell viability in a fermented meat environment will most likely be strain-dependent [72]. Therefore, the choice of appropriate microorganisms to be applied as probiotic in a fermented meat matrix is considered to be critical [74,75].

4.3. Physical Factors

Physical factors that affect probiotic survival include storage temperature, drying conditions or oxygen levels. Cell membranes of probiotics get damaged during freezing process due to mechanical stresses imposed by the development of ice crystals in the external medium (intercellular space) or inside the cells. The size of ice crystals can be reduced by applying rapid freezing rates that result in smaller ice crystals [76,77]. On top of that, the survival of probiotics may be further reduced during thawing where the cells are exposed to osmotic stresses [78].

The fermentation temperature also affects the viability of probiotic microorganisms; with the optimum temperature range for growth for the majority of LAB being within a range of 30–43 °C. Some bacteria, however, such as those of yogurt cultures and L. acidophilus can grow at 45 °C. Usually, temperatures above 45 °C during processing could negatively affect probiotic survival [60]. Species of bifidobacteria isolated from the human intestinal tract such as B. longum subsp. infantis, B. breve, B. bifidum, and B. adolescentis show an optimum growth temperatures in the range of 36–38 °C, whereas B. animalis subsp. lactis can grow at higher temperatures of 41–43 °C [59].

In order to maintain cultures for experimental and industrial uses, drying can be employed to reduce the cost of frozen storage and transportation; i.e., probiotic foods are sometimes dried in order to increase their shelf life at ambient temperature and to reduce the cost of frozen storage at very low temperatures, −20 to −40 °C [79,80]. Several drying methods can be applied however, freeze-drying, spray-drying and vacuum-drying [79,81,82] are the most applicable methods for bacterial culture preservation.

Spray-drying is an economical and flexible method for drying liquid foods; however, its application to preserve probiotic cultures usually entails significant losses in cell viability due to high temperatures, dehydration and osmotic phenomena. Based on several studies, the survival of probiotic cultures during spray-drying depends on several factors such as the species and strain of probiotics used, the drying parameters (outlet air temperature, type of atomization), and the drying and growth medium [60,83]. Freeze-drying, on the other hand, is an expensive process that maintains largely the viability of the probiotic cells [82,84,85].

5. Strategies for Enhanced Probiotic Viability

5.1. Selection of Probiotics

The selection and characterization of new species (e.g., Lactobacillus, Bifidobacterium, Propionibacterium, Faecalibacterium) constitutes a major field of research with respect to the selection of probiotics. The criteria for a strain to be characterized as a probiotic either for food or nutraceutical applications are constantly evolving and developing. These criteria can be roughly divided in four distinct categories: 1. technological, 2. safety, 3. functional and 4. physiological characteristics [60,86,87,88]. Viability during food processing and/or storage and survival after the passage in the upper intestinal transit along with the ability to exert health benefits to the host constitute the most important criteria for the probiotic selection. Most bacteria are unable to survive against the harmful environment during the upper GIT transit, including that of the gastric and duodenum. Hence, selection of the appropriate probiotic strain is a key factor in formulating products with viable probiotic cultures.

Several studies showed losses of 6 to 8 log cfu/g of probiotic bacteria during artificial gastric digestion [89,90,91], implying that the residual probiotic counts are not sufficient to exert health beneficial effects. Vijayakumar, et al. [92] studied the probiotic potential of the lactic acid bacteria L. plantarum KCC-24, that was isolated and characterized from Italian ryegrass (Loliummulti florum) forage [92]. The isolated strain exhibited significant antifungal activity against several strains, it was susceptible to numerous antibiotics, survived in low pH, was resistant to simulated gastric juices and bile salts (0.3% w/v). Also, L. plantarum KCC-24 exhibited good proteolytic activity, potent antioxidant and hydrogen peroxide resistance properties, thus proving to be an excellent probiotic candidate.

Turková, et al. [93] have evaluated eleven strains of Lactobacillus included in the Culture Collection of Dairy Microorganisms (CCDM) for selected probiotic properties such as survival in the gastrointestinal fluids, antimicrobial activity, and competition with non-toxigenic Escherichia coli O157:H7 for adhesion on Caco-2 cells [93]. All strains presented significant antimicrobial activity, whereby the three best performing strains inhibited the growth of at least sixteen indicator pathogenic strains. The degree of competitive inhibition of non-toxigenic E. coli O157:H7 adhesion on the surface of Caco-2 cells was found to be strain-dependent. However, only three of the strains were selected for additional studies of antimicrobial activity, i.e., L. gasseri CCDM 215, L. acidophilus CCDM 149, and L. helveticus CCDM 82 [93].

The effect of carrier food matrix on in vitro gastrointestinal survival and adhesion ability of probiotic L. acidophilus LA-5, B. animalis subsp. lactis BB-12 and P. jensenii 702 were evaluated on dairy products [94]. The carrier food matrix had a significant influence on the in vitro gastrointestinal tolerance of all probiotics upon exposure to low pH (pH 2.0) and 0.3% bile. For instance, Terpou et al. [95,96], reported a significant tolerance improvement in the case of ice cream and a moderate one in the case of yogurts, while Ranadheera et al. [94] observed that in vitro adhesion ability of probiotics was influenced by the carrier food matrix, with fruit yogurt providing the most favorable results [94].

Following an exposure to acidic conditions, B. longum presented an enhanced survival when compared to B. infantis, B. adolescentis and B. bifidum [97]. Amongst various strains of lactobacilli investigated L. acidophilus ATCC 4962, L. casei ASCC 290 and L. casei ASCC 292 were the most acid-tolerant. Live cell density was >107 cfu/mL after 2 h incubation at pH 2.0, whereas L. casei ASCC 1520, L. casei ASCC 1521, L. casei ASCC 279, L. casei ATCC 15820 and L. casei CSCC 2607 were the most acid-sensitive strains with only 104 total cfu/mL remaining after 2 h incubation [98]. Lo Curto, et al. [99] investigated the survival of three commercial probiotic strains (L. casei subsp. shirota, L. casei subsp. immunitas, L. acidophilus subsp. johnsonii) in the human upper GIT using a dynamic gastric model (DGM) of digestion followed by incubation under duodenal conditions. Higher survival was observed in stationary phase for all strains [99]. The L. acidophilus subsp. johnsonii exhibited the highest survival rate in both water and milk. Hence, it is well documented that the ability of the probiotic strain to tolerate gastric acid conditions and the toxicity of bile salts is one of the key factors to be fulfilled in the probiotic selection criteria.

5.2. Strain Adaptation on Food Matrix and Human Microenvironment

Traditionally, probiotic strains are selected based on stress-resistance phenotypes that guarantee their survival through the GIT and their survival rates within each food matrix. Production, storage, and use of LAB impose environmental stress on bacterial cells. Especially during industrial fermentation, LAB encounter a number of stress conditions such as low temperature, low pH, and low water activity [100]. Although the application of physical stress to microorganisms, is the most widely used method to induce cell inactivation and enhance food stability, microorganisms have evolved both physiological and genetic mechanisms to tolerate some extreme conditions in order to survive [71]. This is clearly of major significance to the food industry in relation to antagonism against pathogens or spoilage organisms [101]. Interestingly, the application of different stress conditions to improve the viability and stability of probiotics is of significant interest.

Probiotics sustain several molecular mechanisms to respond the environmental stress encountered either during processing or during ingestion and passage in the GIT. Hence, by elucidating the underlying mechanisms probiotics with high and enhanced viability could be developed. Stress adaptation is one of the strategies to improve the survival of probiotics. This is achieved by pre-treating (preculturing) the bacterial cells at a sublethal stress condition prior to exposure to a more harsh or lethal environment [102]. This approach allows probiotic bacteria to develop adaptive stress responses leading to an increase in their survival compared to those that are directly shifted into the same lethal stress condition [103]. Adaptive responses towards various types of stress, i.e., heat, cold, acid, bile salts, osmotic, oxygen, high pressure and nutrient starvation have been applied for the production of high tolerant probiotic strains [104,105]. These features usually resemble environmental conditions typically encountered by probiotics during human GIT transit, exposure to industrial-scale production protocols and in the food matrix environment [106]. Acid and osmotic stress, occurring from lactic acid production and application of food additives, are the predominant stress factors during yoghurt manufacture and refrigerated storage [107]. Recent advances in post-genomics technologies, i.e., transcriptomics and proteomics, have provided novel insights into the mechanisms by which probiotics counteract environmental stresses [108].

In their various applications in the food and feed industry, LAB can be exposed to osmotic stress when large quantities of salt or sugar are added in the product. Thus, they need to adapt to such a change in their environment in order to survive. Accumulation of compatible solutes (uptake or synthesis) under hyper-osmotic conditions and releasing (or degrading) them under hypo-osmotic conditions comprises one of the possible mechanistic actions. Compatible solutes may also stabilize enzymes and thereby provide protection not only against osmotic stress but also against high temperature, freeze thawing, and drying processing protocols [109,110,111]. Similarly, polyphenols present in the food matrix (e.g., blackcurrant juice) might equally impair or enhance lactobacilli growth depending on the concentration of anthocyanins [112].

Adaptive stress responses in probiotics are also associated with the alteration of various physiological features and structural cell components [113]. It has been reported that for acid tolerance response several mechanisms are linked to pH homeostasis by the proton-translocating F1F0-ATPase, alteration of cell membrane fatty acid composition that leads on the modification of cell membrane properties, the increase of alkalinity of cytoplasm by the activity of arginine deaminase, urease and glutamine decarboxylase and the production of several stress proteins [114]. Furthermore, the response to osmotic stress involves the accumulation of compatible solutes and activation of membrane associated proteins for maintaining turgor pressure of the cell [115].

Several reports have suggested that pre-adaptation can enhance the survival of probiotics in a food system [116,117]. Nevertheless, it is considered that adaptive responses are highly strain-dependent and vary largely according to the type of stress along with other experimental conditions [117]. Settachaimongkon, et al. [118] investigated the effect of preculturing L. rhamnosus GG and B. animalis subsp. lactis BB12 under sublethal stress conditions, on their survival and metabolite formation in set-yoghurt [118]. Both probiotic strains presented adaptive stress responses resulting in viability improvement without any adverse effects on milk acidification.

Osmotic stress is also considered a stress factor related with probiotic viability in food matrices. For example, flow cytometric studies, performed on some lactobacilli exposed to varying levels of sugar concentrations, showed losses of probiotic viability due to osmotic stress [119]. Gandhi and Shah [120] used flow cytometry to evaluate the probiotic cell viability when subjected to varying NaCl concentrations (0–5%) and studied the metabolic state of cells [120]. Double staining of the cells and metabolic activity measurements revealed the degree of cell injury when subjected to NaCl concentrations. They also reported on the variability in salt resistance of three probiotic strains; the salt resistance of L. casei was found to be higher than L. acidophilus and B. longum.

Oxygen stress is another hurdle that probiotic microorganisms may encounter during food production. Oxygen dissolves easily in milk and other liquid foods whereas oxygen permeation through the package could affect probiotic viability [121]. Some studies showed that gradual exposure to increasing concentrations of dissolved oxygen could improve viability in B. longum, L. acidophilus and Bifidobacterium sp. cultures [122,123].

Acid stress adaptation has been originally investigated for food pathogens since it can significantly influence the survival of these bacteria in acidic environments. Such a mechanism, although undesirable for pathogenic organisms, can be also effective in altering the survival of probiotics in acidic matrices and through their passage in the GIT. Several studies have focused on the potential stress response mechanisms of lactobacilli in order to improve their capacity to survive and function under industrial production conditions [124,125]. The effect of heat shock and induction of a stress response in Lactobacillus sp. and further exposure of L. rhamnosus GG cells to pressure pretreatment for improving thermo-tolerance upon exposure at 60 °C was also investigated [126,127,128]. Moreover, application of non-lethal heat shock allows bacteria to tolerate a second heat stress higher in intensity [129]. In another study, salt adaptation of L. paracasei NFBC 338 (0.3 M NaCl for 30 min) improved its viability during spray drying [126,127].

5.3. Selection of Food Packaging Systems

The physical properties of packaging material and packaging techniques could influence the survival of probiotics [130]. The majority of dairy probiotic and other products are stored and sold in the market in plastic packages with high oxygen permeability. Bifidobacteria, in particular, are anaerobes with a high susceptibility to oxygen, therefore a packaging with high oxygen permeability could affect their viability during storage. In this context, several factors such as temperature, relative humidity and crystallinity of the film matrix may affect the permeability of packaging material and thereby alter the probiotic viability [131,132]. Miller, et al. [133] have reported that packaging of yogurt in a container made of a material with good oxygen barrier properties along with an oxygen scavenging agent provides the most favorable environmental conditions for preserving probiotics [133]. Shah [121] reported greater survival of L. acidophilus in yoghurt packed in glass bottles rather than in plastic containers and suggested the use of packaging materials with greater thickness for better survival of both L. acidophilus and bifidobacteria in yoghurt [121]. Cruz, et al. [134] studied the viability of probiotics in yogurts packed in different plastic containers with different oxygen permeability along with the addition of glucose oxidase [134]. As expected, low oxygen permeability rates resulted in higher probiotic viability, even though a higher post-acidification and organic acid production was observed. Therefore, low oxygen permeability in containers favors the survival of probiotic cultures. However, the use of glass containers, having limited oxygen permeability, increases the material cost for probiotic packaging. Alternative packaging methods such as the inclusion of oxygen scavengers or absorbents, vacuum packaging or active packaging with the inclusion of oxygen barrier materials could provide a more cost effective solution [60].

5.4. Addition of Compounds as Probiotic Promoters

Different compounds may be added in probiotic products to act as growth promoters (e.g., sugars, vitamins, minerals, prebiotics) or as protectants against processing conditions (e.g., skim milk powder, whey protein, glycerol, lactose). Also, the use of prebiotics as protectant for probiotic microorganisms has been gaining considerable interest. The prebiotic definition was quite recently revised as “a substrate that is selectively utilized by host microorganisms conferring a health benefit” [135].

Tian, et al. [136] studied the feasibility of casein glycomacropeptide (GMP) hydrolysates as potential prebiotics in yogurt. The growth performance of B. animalis subsp. lactis (Bb12), L. bulgaricus and S. thermophilus in the presence of GMP hydrolysate produced with papain (GHP) was evaluated. The results showed an improvement in the growth of S. thermophilus, but lower effect on the growth of L. bulgaricus. However, the viable count of Bb12 of the yogurt obtained with the addition of 1.5% GHP was about four times higher than that of the control without GHP addition [136]. Shin, et al. [137] found that the viability of commercial Bifidobacterium spp. in skim milk improved by 55.7% after 4 weeks of refrigerated storage when fructo-oligosaccharides (FOS) were added [137]. The addition of oligofructose as prebiotic into yogurt (1.5% w/v) improved the viability of the probiotic organisms during refrigerated storage [138]. Also, inclusion of other protective compounds such as whey protein hydrolysate [139], inulin [140], and whey protein concentrate [141] was found to promote probiotic viability. Akalin, et al. [142] showed that incorporation of FOS in yogurt improved the viability of bifidobacteria [142].

Several of the aforementioned compounds (e.g., glycerol, skim milk powder, whey proteins and sugars) have been also utilized as thermo- or cryo-protectants during the production of probiotics [60]. Capela et al. [138] have reported that addition of glycerol prior to freeze drying reduces the osmotic pressure and therefore assists the adaptation of probiotics in the environmental conditions [138]. Skim milk solids have been reported to form a protective coating on cell wall proteins and stabilize the cell membrane [143]. Several carbohydrates also exert protective effects for probiotic bacteria during freeze drying. Trehalose is a well-known inert (non-reducing disaccharide) cryoprotectant due to its remarkably high glass transition temperature, and its ability to engage in strong ion–dipole interactions and hydrogen bonding with other biomolecules, enabling better survival of L. acidophilus [111].

Innovative delivery strategies based on incorporation of probiotic bacteria into protective matrices, such as whey proteins, proved the ability to provide extra protection for bacteria against environmental stresses. In a recent study Cordeiro et al. [144] evaluated skim milk, previously fermented by a L. casei or by P. freudenreichii strain, with the addition of whey protein isolate (WPI). Their results revealed that supplementation with 30% (w/v) of WPI increased the survival rate of both strains when challenged with acid, bile salts, heat and cold stress, compared to fermented skim milk without the addition of WPI [144]. Enhanced viability of freeze-dried L. plantarum cells using of whey protein microgels has also been presented. The protective effect was further improved with the increase of WPI concentration [145].

The influence of the carrier matrix should be also evaluated to select the appropriate probiotic strains for selective applications. The incorporation of cells in hydrocolloids has already demonstrated great potential in the view of bioactive solutions for functional food. Likewise, novel approaches using edible films and coatings have been shown to improve cell population survival [146,147,148,149]. In a recent work L. rhamnosus GG interplay survivability was studied, comprising selected biopolymers, in the presence of whey protein concentrate (WPC) as a potential vehicle [149]. The authors suggested kappa-carrageenan/locust bean gum and sodium alginate as the most promising performing system. Additionally, they concluded that the inclusion of WPI further increased L. rhamnosus GG stability. A successful example has also been presented by Pavli et al. [150] where Na-alginate edible films were evaluated as vehicles for delivering probiotic bacteria to sliced ham using high pressure processing (HPP). The probiotic-supplemented films were found to be efficient for probiotic delivery on the sliced ham, regardless the previous HPP treatment, since viability was fairly constant (>106 CFU/g) throughout storage time regardless the storage temperature (4 °C, 8 °C, 12 °C) [150].

5.5. Encapsulation of Probiotics

Lately, the most investigated method for improvement of probiotic survival and delivery of bioactive compounds is encapsulation (Figure 3). Probiotics encapsulation is known to enhance stability, facilitate handling and storage of probiotic cultures and protect sensitive probiotic lactic acid bacteria from oxygen, freezing and acidic conditions during production, storage and gastrointestinal transit [151]. So far, several parameters have been identified to affect the efficacy of encapsulation in probiotic protection, the method and the wall materials used, the pH, the initial cell population, the probiotic strain, and the food matrix among others [148,152,153,154].

Figure 3

Schematic presentation of the main advantages of probiotics encapsulation.

5.5.1. Encapsulation Materials

Several materials have been applied for probiotic cells encapsulation, including polysaccharides (alginate, plant/microbial gums, chitosan, starch, k-carrageenan, cellulose acetate phthalate), as well as proteins (gelatin, milk proteins) and fats [155,156,157,158] (Table 1). Based on recent studies the use of gum or biopolymeric matrices can retain the viability of encapsulated probiotic cells compared to free ones, providing in many cases antimicrobial effects [159,160,161,162] against pathogenic or spoilage microorganisms. Casein-based microencapsulation proved to improve the viability of Lactobacillus F19 and Bifidobacterium Bb12 during freeze drying and subsequent storage [163]. Phoem, et al. [164] microencapsulated B. longum using extrusion and emulsion techniques for protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. These results showed that encapsulated B. longum with Eleutherine americana and oligosaccharide extract prepared by the extrusion technique, survived better than that by the emulsion technique under adverse conditions. The viability of encapsulated cells was better than free cells at 65 °C for 15 min [164].

Table 1

Advances in combinations of wall materials and technologies for the microencapsulation of probiotic microorganisms.

Wall Materials	Encapsulation Technology	Microorganism	References
Whey	Agglomeration/Spray-drying	Saccharomyces boulardii	[167]
Whey Protein isolate/Gum Arabic	Complex coacervation	Lactobacillus paraplantarum, Lactobacillus paracasei	[168]
Soy protein isolate (SPI) and high methoxy pectin (HMP)	Complexation	Lactobacillus delbrueckii	[169]
Carboxymethyl-cellulose and chitosan	Crosslinking	Lactobacillus rhamnosus GG	[158]
Cellulose and chitosan	Crosslinking	Lactobacillus rhamnosus GG	[170]
Aguamiel, Ag, or sweet whey, SW, as inner aqueous phase	Double emulsion	Lactobacillus plantarum	[171]
Alginate	Dual aerosol	Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM	[172]
Alginate and Maltodextrin	Dual aerosol/freeze drying or spray drying	Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM	[173]
Whey protein concentrate and pullulan	Electrospinning	Bifidobacterium animalis subsp. lactis Bb12	[174]
Alginate and acidified zein	Electrospraying	Lactobacillus acidophilus	[175]
Alginate–human-like collagen	Electrostatic droplet generation	Bifidobacterium longum BIOMA 5920	[176]
Alginate-gelatin and MgO	Electrospraying	Pediococcus pentosaceus	[177]
Ca-alginate	Electrospraying	Lactobacillus plantrarum	[178]
Ca-alginate and chitosan	Electrospraying	Lactobacillus plantrarum	[179]
Whey protein isolate	Electrospraying	Lactobacillus plantrarum	[180]
Whey protein isolate/whey protein isolate and inulin/whey protein isolate and inulin and persian gum	Εlectrospraying/freeze drying/spray drying	Lactobacillus rhamnosus ATCC 7469	[181]
κ-carrageenan	Emulsification, freeze-drying or extrusion	Lactobacillus plantarum	[182]
Alginate	Emulsification/internal gelation	Bifidobacterium bifidum F-35	[183]
Alginate and pectin and gelatin	Emulsion	Lactobacillus plantarum	[184]
Chickpea protein–alginate	Emulsion	Bifidobacterium adolescentis	[185]
Casein, native whey and/or denatured whey proteins	Emulsion	Lactobacillus rhamnosus GG	[186]
Aluminum carboxymethyl cellulose–rice bran	Emulsion	Lactobacillus reuteri	[187]
Na- alginate (Al), alginate/1% gellan gum alginate/gum Arabic	External ionic gelation	Lactobacillus plantarum DKL 109	[188]
Na-alginate	Extrusion	Lactobacillus paracasei LAFTI® L26, Lactobacillus acidophilus Ki, Bifidobacterium animalis BB-12, Lactobacillus casei -01	[189]
Na-alginate and chitosan	Extrusion	Bifidobacterium pseudocatenulatum G4	[190]
Na-alginate and fructo-oligosaccharides	Extrusion	Lactobacillus casei LC-01 and Lactobacillus casei BGP 93	[191]
Alginate-whey protein	Extrusion	Lactobacillus delbrueckii subsp. Bulgaricus	[192]
Alginate coated with chitosan and gelatin	Extrusion	Lactobacillus plantarum TN9Lactobacillus plantarum TN9	[193]
Legume protein isolate–alginate	Extrusion	Bifidobacterium . adolescentis	[194]
Carrageenan-locust bean gum coated milk microspheres	Extrusion	Lactobacillus bulgaricus	[195]
Alginate–milk	Extrusion	Lactobacillus bulgaricus	[196]
Alginate (ALG) and alginate-psyllium (ALG-PSL)	Extrusion	Lactobacillus acidophilus	[197]
Alginate/chitosan/alginate	Extrusion	Lactobacillus salivarus	[198]
Alginate-skim milk	Extrusion	Lactobacillus reuteri DPC16	[199]
Alginate–chitosan	Extrusion	Enterococcus faecium MC13	[200]
Whey protein isolate	Extrusion	Lactobacillus rhamnosus GG	[201]
Pea protein isolate–alginate	Extrusion	Bifidobacterium adolescentis	[202]
Na-alginate	Extrusion/emulsion	Bifidobacterium. longum	[164]
Na-alginate coated with starch and chitosan	Extrusion	Lactobacillus acidophilus	[157]
Sweet whey and shellac	Fluidized bed microencapsulation	Lactobacillus reuteri	[203]
Gelatin and gum Arabic	Freeze drying	Bifidobacterium lactis	[204]
Na-alginate, gellan gum and skim milk powder	Freeze drying	Lactobacillus kefiranofaciens M1	[205]
Sugarcane bagasse (SB) and sodium alginate (naa)	Immobilization/extrusion	Lactobacillus rhamnosus NRRL 442	[206]
Pectin coated with whey protein heat treated or without heat treatment	Ionotropic gelation and electrostatic interactions	Lactobacillus acidophilus La5	[207]
Chitosan and carboxymethyl cellulose	Layer by layer	Lactobacillus acidophilus	[208]
Chitosan and dextran sulfate	Layer-by-layer technique (lbl) using oppositely charged polyelectrolytes	Saccharomyces boulardii	[209]
Sodium caseinate and gellan gum	Ph- induced gelation	Lactobacillus casei	[210]
Solid lipid microparticles With prebiotics (inulin, polydextrose)	Spray chilling	Lactobacillus acidophilus	[211]
Vegetable fat with lecithin	Spray chilling	Bifidobacterium lactis, Lactobacillus acidophilus	[212]
Gum Arabic and β-cyclodextrin	Spey chilling and spray drying	Lactobacillys acidophilus	[213]
Fructo-oligosaccharide (FOS) and whey proteins	Spray drying	Lactobacillus plantarum MTCC 5422	[214]
Gum Arabic/maltodextrin/whey protein concentrate	Spray drying	Lactobacillus acidophilus	[159]
Slurry fermentation with whey	Spray drying	Lactobacillus reuteri	[215]
Skim milk and whey, maltodextrin, pectin, and arabic gum	Spray drying	Lactobacillus plantarum	[216]
Reconstituted skim milk (RSM) with prebiotics (inulin, oligofructose-enriched inulin, and oligofructose	Spray drying	Bifidobacterium BB-12	[217]
Whey	Spray drying	Bifidobacterium Bb-12	[218]
Whey protein isolate with sodium alginate and denatured whey protein isolate with sodium alginate	Spray drying and freeze drying	Lactobacillus plantarum	[219]
Sweet whey or skim milk	Spray drying	Lactobacillus acidophilus La-5	[220]
Native rice starch and inulin	Spray drying	Lactobacillus rhamnosus	[221]
Maltodextrin	Spray drying	Lactobacillus casei	[222]
Whey protein	Spray drying	Lactobacilus acidophilus, Lactobacillus paracasei L26 and Bifidobacterium animalis BB-12	[223]
Poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid)	Supercritical carbon dioxide	Bifidobacterium longum Bb46	[224]
Alginates-chitosan	Vibrating technology/extrusion	Lactobacillus reuteri DSM 17938	[225]

Encapsulation of bifidobacteria in poly-(vinylpyrrolidone)-poly-(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex microparticles under supercritical conditions was applied by Thantsha, et al. [165]. They reported that the produced microparticles had suitable characteristics for food applications and protected the bacteria in simulated gastrointestinal fluids as well as improved the shelf life for 12 weeks at 30 °C [165]. The strain B. adolescentis (ATCC 15703) was entrapped within microcapsules prepared using 10.00% (w/w) chickpea protein isolates cross-linked with 0.20% (w/v) of genipin, or in the presence of 0.20% (w/v) alginate or k-carrageenan. Overall, the chickpea protein capsule in the presence of 0.10% (w/v) alginate offered the best protection to B. adolescentis in synthetic gastric juice. Poly(d,l-lactic-co-glycolic acid) (PLGA) based microcapsules, containing galacto-oligosaccharides encapsulated into an alginate–chitosan matrix along with a probiotic B. breve strain resulted in enhanced survival of the cells in acidic environment [166].

FOS in combination with whey protein isolate (WPI) or denatured whey protein isolate (DWPI) were also reported as carriers for probiotic strains of L. plantarum strains [214]. The produced microcapsules showed high encapsulation efficacy whereas particle stickiness and aggregation were reduced, along with better storage stability. Recently, Singh et al. [170] entrapped the probiotic strain L. rhamnosus GG in novel cellulose/chitosan-based particles supporting bacterial storage stability. The different particles also presented low toxicity to a colon CaCo-2 cell line [170]. In another study, Yao et al. [177] reported increased survival of P. pentosaceus after encapsulation in microgels with inorganic nanoparticles. More specifically the authors reported that the addition of MgO enhanced the viability of bacteria by filling the pores inside the microgels. Furthermore, it was observed that the presence of MgO-loaded microgels reduced the acid-induced degradation of bacteria by neutralizing the hydrogen ions in the gastric fluids [177]. Incorporation of probiotics to different food products has also been evaluated. Alginate- soy protein isolate (SPI) based hydrogel beads have been also used for incorporating L. plantarum cells into mango juice. Probiotics’ encapsulation showed successful resistance to thermal conditions (72 °C for 90 s) under pasteurization process [226].

5.5.2. Encapsulation Technologies

An effective encapsulation process can be defined as the process by which live cells are packaged within a shell material to offer protection against unfavorable environmental conditions and allowing for their controlled release under intestinal conditions [227]. So far, several methods are available for the encapsulation of probiotics, such as spray drying, extrusion, emulsion or phase separation, freeze drying, ionotropic gelation [228] (Table 2). All these methods have several advantages and disadvantages to offer in probiotic encapsulation. Spray drying for example, can be considered as the most efficient method in terms of cost, equipment availability, particle size, processing volumes and being a continuous process [143,229,230]. However, probiotic cell viability may be significantly influenced by spray drying conditions. Several reviews describe thoroughly the different microencapsulation methods used for probiotic bacteria. Hence, only few and most recent studies will be reported. Lately, complex coacervation has been successfully applied for probiotic encapsulation [168,204,231].

Table 2

Food applications of microencapsulated probiotic bacteria.

Food	Microorganism	Coating Materials	Method	References
Apple juice	Lactobacillus rhamnosus GG	WPI alone and in combination with a modified resistant starch (RS)	Spray drying	[233]
Carrot Juice	Lactobacillus casei	Chitosan-Ca-alginate	Extrusion	[234]
Carrot juice	Lactobacillus acidophilus	Alginate-inulin-xanthan gum	Extrusion	[235]
Cheddar cheese	Bifidobacterium longum	Na-alginate and palmitoylated alginate	(i) droplet extrusion method (ADE) and (ii) emulsion method	[236]
Dry fermented sausages	Lactobacillus reuteri	Alginate	Extrusion	[237]
Fermented milk	Lactobacillus casei ATCC393	Chios mastic gum	Freeze drying	[162]
Fruit juice	Lactobacillus paracasei L26	Alginate	Extrusion	[238]
Fruit juice	Lactobacillus rhamnosus GG	Whey/alginate	Droplet extrusion with coating via electrostatic deposition	[239]
Fruit juices	Bifidobacterium longum, Bifidobacterium breve	poly-γ-glutamic acid	Freeze drying	[240]
Fruit juices	Lactobacillus plantarum and Bifidobacterium longum	Alginate or pectin coated with chitosan, gelatin or glucomannan	Extrusion	[241]
Ice cream	Lactobacillus casei Lc-01 and Bifidobacterium lactis Bb-12	Alginate and Hi-maize resistant starch	Emulsion	[242]
Iranian yogurt drink (Doogh)	Lactobacillus acidophilus LA-5 and Bifidobacterium lactis Bb-12	Alginate	Extrusion	[243]
Kasar cheese	Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum BB-12	Alginate	Emulsion or extrusion	[244]
kefir	Bifidobacterium animals	Sodium alginate	Extrusion	[245]
Mango juice	Lactobacillus plantarum	Calcium-Alginate-Soy Protein Isolate	Gelation	[226]
Mozzarella cheese	Lactobacillus paracasei ssp. paracasei LBC-1	Alginate	Extrusion	[246]
Oaxaca cheese	Lactobacillus plantarum	Aguamiel, Ag, or sweet whey, SW, as inner aqueous phase	Double emulsion	[171]
Pecorino cheese	L. acidophilus, B. longum and B. lactis	Na-alginate	Extrusion	[247]
Pecorino cheese	Lactobacillus acidophilus and a mix of Bifidobacterium longum and Bifidobacterium lactis	Alginate	Extrusion	[248]
Pomegranate juice	L actobacillus plantarum	Alginate beads coating with double layer Chitosan	Extrusion	[249]
White-brined cheese	Bifidobacterium bifidum BB-12 and Lactobacillus acidophilus LA-5	Alginate	Emulsion or extrusion	[250]
Yogurt	Lactobacillus acidophilus LA-5	Pectin – Whey protein	Ionic gelation and complexation	[251]
Yogurt	Bifidobacterium bifidum F-35	Whey/alginate	Extrusion	[252]
Yogurt	Lactobacillus acidophilus ATCC 4356	Alginates	Extrusion	[246]
Yogurt	Bifidobacterium animalis subsp. lactis Bb12 and Lactobacillus rhamnosus	Alginate	Extrusion	[253]
Yogurt	Lactobacillus plantarum	Sodium alginate or pectin, coated with sodium alginate or chitosan	Extrusion	[91]
Yogurt	Lactobacillus casei	Sodium alginate (A), amidated low-methoxyl pectin (P), and blends	Extrusion	[254]
Yogurt	Lactobacillus acidophilus	alginate and chitosan	Extrusion	[255]
Yogurt—Ice cream	Lactobacillus acidophilus La-5	Na-alginate	Extrusion	[256]

A mixture of L. acidophilus LA-5, B. animalis subsp. lactis BB-12 and a novel potential probiotic strain P. jensenii 702, was spray dried (inlet temperature 195 °C and outlet temperature 85 °C) and the viability during storage was evaluated. Spray drying resulted in a significant reduction in the viability of all three probiotics. All three probiotics were able to maintain satisfactory viability levels (106–108 cfu/g) after spray drying, still their viability declined dramatically after storage at 30 °C. However, lactobacilli and propionibacteria remained virtually unaffected under storage at 4 °C, satisfying the recommendations regarding the level of viable cells in probiotic foods [232].

Two wall materials (native rice starch, NRS, and inulin, IN) without cross-linking agents were used as protectants of L. rhamnosus during spray-drying by determining the viability of the microorganism under two storage conditions [221]. The use of both colloidal prebiotics (NRS and IN) without a bonding agent was found suitable to protect L. rhamnosus during spray drying. The varying morphological and physicochemical properties conferred by each hydrocolloid, along with the different degree of protection provided by each of these encapsulants, suggested that the release of the microbial cells in the gastrointestinal system or in the food matrix could potentially differ depending on the wall material used in the encapsulation process. The viability and stability results indicated that both hydrocolloids protected substantially the L. rhamnosus cells upon spray-drying.

Fabrication of hydrogel Ca-alginate/chitosan microcapsules has been employed by Zaeim et al. [179] using electrospray techniques. In that study it was revealed that the different microcapsule matrices played an important role on survival of bacteria during storage and acid exposure. It was also presented that the outer layer of chitosan on Ca-alginate microcapsules was more efficient to protect bacteria at low pH environments [179].

Electro-spraying microencapsulation was also applied for L. plantarum, whereby cell viability was improved under refrigeration storage, simulation of gastric and intestinal fluids [178]. Recently, optimization of electrospraying conditions for probiotic encapsulation has been performed by Gomez-Mascaraque et al. [180]. The survival of protected L. plantarum evaluated during simulated in vitro gastrointestinal digestion used WPC matrix. The authors observed low viability losses and high product yields during digestion process that increased with the voltage, surfactant and prebiotic concentrations. Moreover, they presented enhanced protection of the food ingredient during storage at high relative humidity i.e., 53% and 75% [180].

6. Development of Synbiotics

As previously stated, the definition of prebiotic was recently restructured to “a substrate that is selectively utilized by host microorganisms conferring a health benefit” [134]. Hence, the definition is not limited only to fermentable carbohydrates but can also include components like polyphenols and polyunsaturated fatty acids. For instance, polyphenols might reach the colon and be further metabolized by gut microbiota [257]. Prebiotic compounds were focused to convey a health effect in the gut, reduced cardiometabolic risk and mental health (e.g., enhanced cognition). However, the new definition includes the modulation of any host microbial ecosystem [135]. Also, prebiotics will target possible health effects by modulating populations of Roseburia, Eubacterium and/or Faecalibacterium spp. populations apart from Lactobacillus and Bifidobacterium [135].

Fructans, FOS and inulin, as well as galacto-oligosaccharides (GOS) and lactulose, are the most commonly used compounds as prebiotics [258]. Nonetheless, an ample range of carbohydrates with different monosaccharide content and configuration of glycosidic linkages could exhibit potential prebiotic effect. Novel prebiotic compounds could derive from natural and renewable resources or synthesized enzymatically [259]. Dietary fibers and their hydrolysis products are becoming an emerging source of new ingredients with potential prebiotic activity.

Implementation of “-omic” approaches could elucidate underlying mechanisms of interaction between gut microbiota, including cross-feeding phenomena along with selectivity and specificity on fermentable carbohydrates and other components. Hence, the use of prebiotics is gaining considerable interest, as they target to sustain a healthy microbiome or restore microbial dysbiosis [260,261].

A synbiotic includes the combination of a probiotic and a prebiotic and should target to enhance the survival and the implantation of the probiotic in the GIT to promote beneficial bacterial groups [262] (Figure 4). Following the definition, a synbiotic can have either complementary or synergistic action. In the first case, the prebiotic is independently selected to enhance indigenous beneficial microbiota and the probiotic is selected for a targeted biological action. On the other hand, in the latter case, the prebiotic is chosen to support specifically the growth of the selected probiotic. Hence, the prebiotic is included to be selectively fermented by the probiotic strain; regardless the beneficial impact on the population of other bacteria [262]. The development of synbiotics is emerging to be of paramount importance as it can be used as a supplement in food and nutraceutical applications. Nutraceuticals can be used as dietary supplements or functional foods, as they are food ingredients or sourced from food products that, apart from the basic original nutritional value, provide extra benefits (e.g., chronic disease prevention, improving health) [263,264,265].

Figure 4

Beneficial effects of synbiotics.

Synbiotics, in a technological viewpoint, were also designed to overcome difficulties such as cell survival in the GIT. Likewise, the development of a combination in a single product could ensure an effective formulation, compared to the activity of the probiotic or prebiotic alone. Features like long-term stability during the shelf-life of food, drinks and resistance of probiotics to processing also exhibit a positive effect on the use of synbiotics.

Most synbiotic formulations include either yogurts or dairy drinks, however new products are under ongoing design. For instance, the development of synbiotic milk chocolate using encapsulated L. casei cells has been reported by Mandal et al. [266]. Milk chocolates presented a promising food delivery system for probiotics, whereby cell viability was enhanced with inulin. Studies conducted in vivo in mice fed with synbiotic milk chocolate led to an increase in faecal lactobacilli, decreased faecal coliforms and β-glucuronidase activity [266]. Criscio et al. [267] developed prebiotic, probiotic and synbiotic ice creams, whereby the synbiotic was formulated with inulin and Lactobacillus strains. Viable counts to ensure probiotic dosage were documented after frozen storage, where organoleptic characteristics were also maintained [267].

Encapsulation of food bioactives in micro- and nanoparticles via nanoscale control of food molecules could modify and enhance desired characteristics to develop functional foods. Encapsulation approaches aiming to develop synbiotics have been already applied in the literature [268]. Other novel approaches have been also presented, such as the non-dairy synbiotic beverages [269]. In that study, selected probiotic LAB were encapsulated by incorporating into rice-berry malt extract (RME). The aim was the production of a lactose free product through alginate hydrogel encapsulation including inulin. The results were quite promising since RME medium supported the growth of the selected LAB and alginate hydrogel significantly improved their survivability in the GIT. Finally, the authors suggested that the synbiotic beverage maintained high concentrations of L. plantarun cell under cool storage for 2 weeks.

Co-encapsulation of synbiotics usually occurs by employing alginate as a matrix. Alginate gels are stable at low pH values and can be swelled at higher pH values (as in intestinal environments) whereby release of the cells enhanced viability [270]. Atia et al., [271] studied an alginate-inulin synbiotic co-encapsulation of probiotic to target delivery in the colon as a site action. The results revealed that formulations containing inulin improved muco-adhesion properties of the probiotic beads, increasing also their protection from the acidic environment [271]. More recently synbiotic encapsulation of L. plantarum was also evaluated, using alginate-arabinoxylan composite microspheres, whereby encapsulation efficiency along with survival and storage stability were enhanced [268]. The same probiotic species have also been studied by Vaziri et al. [184] applying co-microencapsulation with DHA fatty acid in alginate-pectin-gelatin biocomposites.

7. Future Prospects

Incorporation of probiotics in foods at an industrial scale encompasses several microbiological, technological and economical challenges. Further research is required on the design of appropriate technologies, carrier matrices, and selection of bacterial strains to promote the survival of the bacterial cells under varying processing conditions (e.g., heat, osmotic and oxygen stresses) as well as during their passage through the upper gastrointestinal tract. Encapsulation of probiotic bacteria constitutes an approach that can be applied in a number of foods to achieve a wide variety of functional features. Nevertheless, several methods employed to improve probiotic viability such as microencapsulation induce an extra cost to food production process. This added manufacturing cost must be within acceptable limits to remain competitive in the globalized market of functional products.

Identifying the appropriate bacterial strains and the microencapsulation materials and process pose important challenges that need to be further addressed. It is of paramount importance that the microencapsulation methods are efficient, sustainable and environmentally friendly. Future research should focus on the optimization of probiotic cells use, considering as key factors safety and ecological production. Encapsulation materials that are not GRAS certified (e.g., chitosan) or of animal origin (e.g., gelatin) raise consumers’ concerns and remain a critical issue. On the other hand, the large size of microbial cells (1–3 μm), along with the high number of cells, can lead to large capsule sizes that may negatively influence the sensory properties of food.

Another issue that should be also considered is the safety of bacteria inclusion into food formulation, regardless that LAB strains are predominantly classified as GRAS. The origin of the strain, the delivery dose, the method and the period of supplementation, but also end users are included to assess safety. Groups of increased attention are infants, the elderly and people with compromised immune system. For instance, the expression of antibiotic resistant genes from probiotic strains combined with the indigenous gene expression of gut microbiota might result in DNA exchange between bacteria and human intestine cells as a secondary impact [272]. Ultimately, it is unequivocal that consumers should be directed towards making more conscious choices. To this end, knowledge transfer between academia, consumers, manufacturers and stakeholders is a prerequisite to avoid misinterpretation of solid scientific outcomes on the beneficial effects of probiotics.

At an industrial scale, there are several difficulties that hinder the application of microencapsulation. Different microencapsulation technologies are not yet fully exploited and require additional experimental work to be successfully implemented in real food matrices. Research efforts should be directed towards the enhancement of the properties of the microcapsules, expanding the currently available methods and overcoming the technological challenges for the production of novel functional foods. Indeed, new technical innovations are being introduced continuously. Companies using microencapsulation technologies do not hold great expectations for new, high-volume and enhanced beneficial value products. Within this context, the food industry will need further expertise to effectively introduce the most promising technologies in the evolution of the next food product generation.

On the other hand, development of synbiotics indicates a promising approach in the design of functional foods that will ultimately assist to modulate gut microbiota and convey health effects. One of the targets is to increase and maintain the cell viability after the passage through stomach and small intestine, aiming to compete with indigenous microbiota. Another major challenge will be the selection of the prebiotic candidate that will target to enhance the ability of the probiotic strain to survive in the GIT. Under this viewpoint, it is critical to investigate the selective fermentation of potential prebiotic compounds and develop novel and sustainable formulations, whereby renewable resources could be evaluated as onset materials to extract potential prebiotics.