Literature DB >> 22307289

Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene.

Jianlong He1, Kouta Sakaguchi, Tohru Suzuki.   

Abstract

For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.

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Year:  2012        PMID: 22307289      PMCID: PMC3318838          DOI: 10.1128/AEM.07093-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  27 in total

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