Literature DB >> 7071021

Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

H Ito, Y Ike, S Ikuta, K Itakura.   

Abstract

A simple solid phase method for the synthesis of oligodeoxyribonucleotides has been developed using the phosphotriester approach. Mononucleotide coupling units are sequentially added to the polystyrene copolymer with 1% divinylbenzene and two kinds of oligonucleotides, d(CACGACCCCTCCACGT) and d(AACTGGTATTACTGGGCG), are synthesized in a relatively high yield. One cycle of the mononucleotide addition is about 70 minutes, and this method is particularly suitable for the automation of the synthesis upon availability of an automatic synthesizer.

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Year:  1982        PMID： 7071021      PMCID： PMC320564          DOI： 10.1093/nar/10.5.1755

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Letter: Phosphite coupling procedure for generating internucleotide links.

Authors:  R I Letsinger; J L Finnan; G A Heavner; N B Lunsford
Journal:  J Am Chem Soc       Date:  1975-05-28       Impact factor: 15.419

2.  Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

Authors:  M J Gait; M Singh; R C Sheppard; M D Edge; A R Greene; G R Heathcliffe; T C Atkinson; C R Newton; A F Markham
Journal:  Nucleic Acids Res       Date:  1980-03-11       Impact factor: 16.971

3.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

4.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

5.  Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

6.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

7.  Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.

Authors:  A F Markham; M D Edge; T C Atkinson; A R Greene; G R Heathcliffe; C R Newton; D Scanlon
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

8.  Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.

Authors:  K Miyoshi; R Arentzen; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

9.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971

10.  Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).

Authors:  T Horn; M P Vasser; M E Struble; R Crea
Journal:  Nucleic Acids Symp Ser       Date:  1980
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  75 in total

1.  Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen.

Authors:  J Miyazaki; E Appella; K Ozato
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

2.  Interaction of the restriction endonuclease ScaI with its substrates.

Authors:  K Kita; N Hiraoka; F Kimizuka; A Obayashi; H Kojima; H Takahashi; H Saito
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

3.  Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms.

Authors:  C J Herbert; M Sarwar; S S Ner; I G Giles; M Akhtar
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857

4.  AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.

Authors:  K Kita; N Hiraoka; A Oshima; S Kadonishi; A Obayashi
Journal:  Nucleic Acids Res       Date:  1985-12-20       Impact factor: 16.971

5.  Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides.

Authors:  H Inoue; Y Hayase; A Imura; S Iwai; K Miura; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

6.  A simple and convenient synthesis of 3'-5'- or 2'-5'-linked oligoribonucleotide by polymerization of unprotected ribonucleoside using phosphorus tris-azole.

Authors:  T Shimidzu; K Yamana; N Kanda; S Maikuma
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

7.  Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.

Authors:  P J O'Hara; F M Horodyski; S T Nichol; J J Holland
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

8.  The presence of cysteine in the cytoplasmic domain of the vesicular stomatitis virus glycoprotein is required for palmitate addition.

Authors:  J K Rose; G A Adams; C J Gallione
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

9.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

10.  Effect of amino acid substitutions on the catalytic and regulatory properties of aspartate transcarbamoylase.

Authors:  E A Robey; S R Wente; D W Markby; A Flint; Y R Yang; H K Schachman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205
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