Literature DB >> 7465420

Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.

K Miyoshi, R Arentzen, T Huang, K Itakura.   

Abstract

Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, dCpCpApGpTpCpApCpGpApCpGpTpTpGpT, were synthesized by the phosphotriester method using di and trinucleotide blocks as coupling units.

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Year:  1980        PMID： 7465420      PMCID： PMC324317          DOI： 10.1093/nar/8.22.5507

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Tert-butoxycarbonylaminoacyl-4-(oxymethyl)-phenylacetamidomethyl-resin, a more acid-resistant support for solid-phase peptide synthesis.

Authors:  A R Mitchell; B W Erickson; M N Ryabtsev; R S Hodges; R B Merrifield
Journal:  J Am Chem Soc       Date:  1976-11-10       Impact factor: 15.419

2.  The monitoring of reactions in solid-phase peptide synthesis with picric acid.

Authors:  B F Gisin
Journal:  Anal Chim Acta       Date:  1972-01       Impact factor: 6.558

3.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

4.  Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

5.  Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

6.  Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Miyake; T Hozumi; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

7.  Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.

Authors:  V K Potapov; V P Veiko; O N Koroleva; Z A Shabarova
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
  7 in total
  30 in total

1.  Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Authors:  A Ono; T Ueda
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

2.  Continuing coevolution of virus and defective interfering particles and of viral genome sequences during undiluted passages: virus mutants exhibiting nearly complete resistance to formerly dominant defective interfering particles.

Authors:  N J DePolo; C Giachetti; J J Holland
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

3.  Studies on nucleic acid interactions. I. Stabilities of mini-duplexes (dG2A4XA4G2-dC2T4YT4C2) and self-complementary d(GGGAAXYTTCCC) containing deoxyinosine and other mismatched bases.

Authors:  Y Kawase; S Iwai; H Inoue; K Miura; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1986-10-10       Impact factor: 16.971

4.  Structure and gene expression of the E. coli Mn-superoxide dismutase gene.

Authors:  Y Takeda; H Avila
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971

5.  Cloning and sequence analysis of a cDNA encoding rat preprocholecystokinin.

Authors:  R J Deschenes; L J Lorenz; R S Haun; B A Roos; K J Collier; J E Dixon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

6.  A large fragment approach to DNA synthesis: total synthesis of a gene for the protease inhibitor eglin c from the leech Hirudo medicinalis and its expression in E. coli.

Authors:  H Rink; M Liersch; P Sieber; F Meyer
Journal:  Nucleic Acids Res       Date:  1984-08-24       Impact factor: 16.971

7.  Cloning and expression of the 1.3S biotin-containing subunit of transcarboxylase.

Authors:  V L Murtif; C R Bahler; D Samols
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

8.  Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

Authors:  S Inouye; X Soberon; T Franceschini; K Nakamura; K Itakura; M Inouye
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

9.  Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.

Authors:  P J O'Hara; F M Horodyski; S T Nichol; J J Holland
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

10.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971
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