Literature DB >> 190591

Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

J Stawinski, T Hozumi, S A Narang, C P Bahl, R Wu.   

Abstract

The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.

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Year:  1977        PMID： 190591      PMCID： PMC342437          DOI： 10.1093/nar/4.2.353

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Recognition sequence of restriction endonuclease III from Hemophilus influenzae.

Authors:  R Old; K Murray; G Boizes
Journal:  J Mol Biol       Date:  1975-02-25       Impact factor: 5.469

2.  Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.

Authors:  G A Wilson; F E Young
Journal:  J Mol Biol       Date:  1975-09-05       Impact factor: 5.469

3.  Improved triester approach for the synthesis of pentadecathymidylic acid.

Authors:  K Itakura; N Katagiri; C P Bahl; R H Wightman; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

4.  Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli.

Authors:  K Itakura; N Katagiri; S A Narang; C P Bahl; K J Marians; R Wu
Journal:  J Biol Chem       Date:  1975-06-25       Impact factor: 5.157

5.  Synthesis of the anticodon loop of Escherichia coli methionine transfer ribonucleic acid.

Authors:  T Neilson; E S Werstink
Journal:  J Am Chem Soc       Date:  1974-04-03       Impact factor: 15.419

6.  5 s RNA conformation. Studies of its partial T 1 ribonuclease digestion by gel electrophoresis and two-dimensional thin-layer chromatography.

Authors:  A D Mirzabekov; B E Griffin
Journal:  J Mol Biol       Date:  1972-12-30       Impact factor: 5.469

7.  The nucleotide sequence of the lac operator.

Authors:  W Gilbert; A Maxam
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

8.  Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors:  G G Brownlee; F Sanger
Journal:  Eur J Biochem       Date:  1969-12

9.  Deoxy oligonucleotide synthesis via the triester method.

Authors:  J C Catlin; F Cramer
Journal:  J Org Chem       Date:  1973-01-26       Impact factor: 4.354

10.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971
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  56 in total

1.  A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approach.

Authors:  A K Sood; S A Narang
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

2.  Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B.

Authors:  D Fong; D H Calhoun; W T Hsieh; B Lee; R D Wells
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

3.  Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Authors:  E de Vroom; A Fidder; J E Marugg; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

4.  Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support.

Authors:  T Tanaka; T Sakata; K Fujimoto; M Ikehara
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

5.  Synthesis and properties of 5'-triphosphoryl 2'-5' oligoadenylates (2-5A), and a general method for synthesis of 3', 5'-bisphosphorylated oligonucleotides.

Authors:  M Ikehara; K Oshie; A Hasegawa; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1981-04-24       Impact factor: 16.971

6.  A simple and convenient synthesis of 3'-5'- or 2'-5'-linked oligoribonucleotide by polymerization of unprotected ribonucleoside using phosphorus tris-azole.

Authors:  T Shimidzu; K Yamana; N Kanda; S Maikuma
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

7.  Synthesis, complete 1H assignments and conformations of the self-complementary hexadeoxyribonucleotide [d(CpGpApTpCpG)]2 and its fragments by high field NMR.

Authors:  J W Lown; C C Hanstock; R C Bleackley; J L Imbach; B Rayner; J J Vasseur
Journal:  Nucleic Acids Res       Date:  1984-03-12       Impact factor: 16.971

8.  General scheme of the phosphotriester condensation in the oligodeoxyribonucleotide synthesis with arylsulfonyl chlorides and arylsulfonyl azolides.

Authors:  V F Zarytova; D G Knorre
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

9.  Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

Authors:  E Ohtsuka; Y Taniyama; R Marumoto; H Sato; H Hirosaki; M Ikehara
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

10.  Chemical synthesis of tridecanucleoside dodecaphosphate sequence of SV40 DNA.

Authors:  J B Chattopadhyaya; C B Reese
Journal:  Nucleic Acids Res       Date:  1980-05-10       Impact factor: 16.971
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