Literature DB >> 7465417

Simplications in the synthesis of short oligonucleotide blocks.

C Broka, T Hozumi, R Arentzen, K Itakura.   

Abstract

A rapid and convenient procedure has been developed for the synthesis of fully protected mono, di and trideoxyribonucleotides utilizing an aryl phosphoroditriazolide. It affords advantages over coupling strategies employing condensing reagents, such as 2,4,6-triisopropylbenzenesulfonyl tetrazolide in preparing small oligonucleotides and is relatively free of the drawbacks inherent in other approaches using bifunctional phosphorylating reagents. In particular, the synthesis of trinucleotide blocks without purification at the dimer stage is described.

Entities:  

Mesh：

Substances：

Year:  1980        PMID： 7465417      PMCID： PMC324314          DOI： 10.1093/nar/8.22.5461

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Improved triester approach for the synthesis of pentadecathymidylic acid.

Authors:  K Itakura; N Katagiri; C P Bahl; R H Wightman; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

2.  Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.

Authors:  H M Hsiung; R Brousseau; J Michniewicz; S A Narang
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

3.  Deoxy oligonucleotide synthesis via the triester method.

Authors:  J C Catlin; F Cramer
Journal:  J Org Chem       Date:  1973-01-26       Impact factor: 4.354

4.  Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.

Authors:  J H van Boom; P M Burgers; G van der Marel; C H Verdegaal; G Wille
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

5.  The use of barium salts of protected deoxyribonucleoside-3' p-chlorophenyl phosphates for construction of oligonucleotides by the phosphotriester method: high-yield synthesis of dinucleotide blocks.

Authors:  G R Gough; K J Collier; H L Weith; P T Gilham
Journal:  Nucleic Acids Res       Date:  1979-12-11       Impact factor: 16.971

6.  Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method.

Authors:  K L Agarwal; F Riftina
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

7.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971
  7 in total
  29 in total

1.  Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Authors:  A Ono; T Ueda
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

2.  Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

Authors:  S V Suggs; R B Wallace; T Hirose; E H Kawashima; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

Review 3.  Recent developments in the chemical synthesis of polynucleotides.

Authors:  E Ohtsuka; M Ikehara; D Söll
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

4.  Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside.

Authors:  H Inoue; A Imura; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1985-10-11       Impact factor: 16.971

5.  Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides.

Authors:  H Inoue; Y Hayase; A Imura; S Iwai; K Miura; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

6.  A simple and convenient synthesis of 3'-5'- or 2'-5'-linked oligoribonucleotide by polymerization of unprotected ribonucleoside using phosphorus tris-azole.

Authors:  T Shimidzu; K Yamana; N Kanda; S Maikuma
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

7.  Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

Authors:  E Ohtsuka; Y Taniyama; R Marumoto; H Sato; H Hirosaki; M Ikehara
Journal:  Nucleic Acids Res       Date:  1982-04-24       Impact factor: 16.971

8.  Molecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors.

Authors:  R A Zivin; J H Condra; R A Dixon; N G Seidah; M Chrétien; M Nemer; M Chamberland; J Drouin
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2'-O-tetrahydrofuranyl-5'-O-dimethoxytritylnucleosides.

Authors:  E Ohtsuka; A Yamane; M Ikehara
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  The synthesis of blocked triplet-phosphoramidites and their use in mutagenesis.

Authors:  A Ono; A Matsuda; J Zhao; D V Santi
Journal:  Nucleic Acids Res       Date:  1995-11-25       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.