Literature DB >> 7443540

Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

M J Gait, M Singh, R C Sheppard, M D Edge, A R Greene, G R Heathcliffe, T C Atkinson, C R Newton, A F Markham.   

Abstract

A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.

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Year:  1980        PMID： 7443540      PMCID： PMC323974          DOI： 10.1093/nar/8.5.1081

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Letter: Polyamide supports for polypeptide synthesis.

Authors:  E Atherton; D L Clive; R C Sheppard
Journal:  J Am Chem Soc       Date:  1975-10-29       Impact factor: 15.419

2.  The use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach.

Authors:  N Katagiri; K Itakura; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

3.  Solid support synthesis of oligothymidylates using phosphorochloridates and 1-alkylimidazoles.

Authors:  R C Pless; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1975-06       Impact factor: 16.971

4.  A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approach.

Authors:  A K Sood; S A Narang
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

5.  Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.

Authors:  E Kaiser; R L Colescott; C D Bossinger; P I Cook
Journal:  Anal Biochem       Date:  1970-04       Impact factor: 3.365

6.  A new polymer-support method for the synthesis of ribooligonucleotide.

Authors:  K F Yip; K C Tsou
Journal:  J Am Chem Soc       Date:  1971-06-30       Impact factor: 15.419

7.  Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

8.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

9.  Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

10.  The mechanism of the chemical synthesis of oligonucleotides and its synthetic consequences.

Authors:  D G Knorre; V F Zarytova
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971
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  46 in total

1.  Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.

Authors:  R T Pon; S Yu
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  Synthesis of cyclic oligodeoxyribonucleotides via the 'filtration' approach.

Authors:  M V Rao; C B Reese
Journal:  Nucleic Acids Res       Date:  1989-10-25       Impact factor: 16.971

3.  The isolation and characterization of a cDNA clone encoding Lupinus angustifolius root nodule glutamine synthetase.

Authors:  M R Grant; A Carne; D F Hill; K J Farnden
Journal:  Plant Mol Biol       Date:  1989-11       Impact factor: 4.076

4.  Analysis of T cell receptor beta chains in rat thymus, and rat C alpha and C beta sequences.

Authors:  M Morris; A N Barclay; A F Williams
Journal:  Immunogenetics       Date:  1988       Impact factor: 2.846

5.  Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12.

Authors:  K L Roland; F E Powell; C L Turnbough
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

6.  Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.

Authors:  D E Woods; A F Markham; A T Ricker; G Goldberger; H R Colten
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

7.  HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.

Authors:  G M Bonora; C L Scremin; F P Colonna; A Garbesi
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

8.  Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance.

Authors:  C R Sharpe; A Sidoli; C S Shelley; M A Lucero; C C Shoulders; F E Baralle
Journal:  Nucleic Acids Res       Date:  1984-05-11       Impact factor: 16.971

9.  Isolation of the human HDL apoprotein A1 gene.

Authors:  C C Shoulders; F E Baralle
Journal:  Nucleic Acids Res       Date:  1982-08-25       Impact factor: 16.971

10.  Color coded triarylmethyl protecting groups useful for deoxypolynucleotide synthesis.

Authors:  E F Fisher; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971
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