Literature DB >> 2997733

Interaction of the restriction endonuclease ScaI with its substrates.

K Kita, N Hiraoka, F Kimizuka, A Obayashi, H Kojima, H Takahashi, H Saito.   

Abstract

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.

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Year:  1985        PMID： 2997733      PMCID： PMC322019          DOI： 10.1093/nar/13.19.7015

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

2.  Sequence-specific recognition of double helical nucleic acids by proteins.

Authors:  N C Seeman; J M Rosenberg; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

3.  Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate.

Authors:  P H Greene; M S Poonian; A L Nussbaum; L Tobias; D E Garfin; H W Boyer; H M Goodman
Journal:  J Mol Biol       Date:  1975-12-05       Impact factor: 5.469

4.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

5.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

6.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

7.  The interaction of the EcoRI restriction endonuclease with its substrate. A physico-chemical study employing natural and synthetic oligonucleotides and polynucleotides.

Authors:  M Goppelt; A Pingoud; G Maass; H Mayer; H Köster; R Frank
Journal:  Eur J Biochem       Date:  1980-02

8.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

9.  Escherichia coli mutants deficient in the production of alkaline phosphatase isozymes.

Authors:  A Nakata; M Yamaguchi; K Izutani; M Amemura
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

10.  Synthesis of deoxyoligonucleotides containing 7-deazaadenine: recognition and cleavage by restriction endonuclease Bgl II and Sau 3AI (nucleosides and nucleotides Part 55).

Authors:  A Ono; M Sato; Y Ohtani; T Ueda
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971
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  7 in total

1.  Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.

Authors:  V Butkus; L Petrauskiene; Z Maneliene; S Klimasauskas; V Laucys; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

2.  Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Authors:  A Ono; T Ueda
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

3.  Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease.

Authors:  N Bischofberger; P G Ng; T R Webb; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

4.  Analysis of substrate specificity of the PaeR7 endonuclease: effect of base methylation on the kinetics of cleavage.

Authors:  S S Ghosh; P S Obermiller; T J Kwoh; T R Gingeras
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

5.  Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.

Authors:  A Fliess; H Wolfes; F Seela; A Pingoud
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

6.  Synthesis of decadeoxyribonucleotides containing 5-modified uracils and their interactions with restriction endonucleases Bgl II, Sau 3AI and Mbo I (nucleosides and nucleotides 82).

Authors:  T Hayakawa; A Ono; T Ueda
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

7.  Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases.

Authors:  Hana Macícková-Cahová; Michal Hocek
Journal:  Nucleic Acids Res       Date:  2009-12       Impact factor: 16.971
  7 in total

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