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Literature DB >> 29687312

Production of isotope-labeled proteins in insect cells for NMR.

Bastian Franke¹, Christian Opitz¹, Shin Isogai¹, Anne Grahl¹, Leonildo Delgado¹, Alvar D Gossert², Stephan Grzesiek³.

Abstract

Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.

Entities: Species

Keywords: Abelson kinase; Algal extract; Baculovirus; Deuteration; GPCR; Yeast extract

Mesh：

Substances：

Year: 2018 PMID： 29687312 DOI： 10.1007/s10858-018-0172-7

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

34 in total

1. Isotope labeling of proteins in insect cells.

Authors: Lukasz Skora; Binesh Shrestha; Alvar D Gossert
Journal: Methods Enzymol Date: 2015-06-26 Impact factor: 1.600

2. Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.

Authors: André Strauss; Francis Bitsch; Gabriele Fendrich; Patrick Graff; René Knecht; Bernd Meyhack; Wolfgang Jahnke
Journal: J Biomol NMR Date: 2005-04 Impact factor: 2.835

3. An improved protocol for amino acid type-selective isotope labeling in insect cells.

Authors: Yaqun Zhang; Hui Wei; Dianlin Xie; Deepa Calambur; Andrew Douglas; Mian Gao; Frank Marsilio; William J Metzler; Nicolas Szapiel; Ping Zhang; Mark R Witmer; Luciano Mueller; David Hedin
Journal: J Biomol NMR Date: 2017-07-15 Impact factor: 2.835

4. The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus.

Authors: David J Wasilko; S Edward Lee; Kim J Stutzman-Engwall; Beverly A Reitz; Thomas L Emmons; Karl J Mathis; Michael J Bienkowski; Alfredo G Tomasselli; H David Fischer
Journal: Protein Expr Purif Date: 2009-01-11 Impact factor: 1.650

5. Robust and low cost uniform (15)N-labeling of proteins expressed in Drosophila S2 cells and Spodoptera frugiperda Sf9 cells for NMR applications.

Authors: Annalisa Meola; Célia Deville; Scott A Jeffers; Pablo Guardado-Calvo; Ieva Vasiliauskaite; Christina Sizun; Christine Girard-Blanc; Christian Malosse; Carine van Heijenoort; Julia Chamot-Rooke; Thomas Krey; Eric Guittet; Stéphane Pêtres; Félix A Rey; François Bontems
Journal: J Struct Biol Date: 2014-08-28 Impact factor: 2.867

6. Established insect cell line from the cabbage looper, Trichoplusia ni.

Authors: W F Hink
Journal: Nature Date: 1970-05-02 Impact factor: 49.962

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Authors: I Kusumoto
Journal: J Nutr Date: 2001-09 Impact factor: 4.798

8. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli.

Authors: V A Luckow; S C Lee; G F Barry; P O Olins
Journal: J Virol Date: 1993-08 Impact factor: 5.103

9. Backbone NMR resonance assignment of the Abelson kinase domain in complex with imatinib.

Authors: Navratna Vajpai; André Strauss; Gabriele Fendrich; Sandra W Cowan-Jacob; Paul W Manley; Wolfgang Jahnke; Stephan Grzesiek
Journal: Biomol NMR Assign Date: 2008-03-05 Impact factor: 0.746

10. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae).

Authors: J L Vaughn; R H Goodwin; G J Tompkins; P McCawley
Journal: In Vitro Date: 1977-04

18 in total

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Authors: Jerome Boisbouvier; Lewis E Kay
Journal: J Biomol NMR Date: 2018-07 Impact factor: 2.835

Review 2. GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures.

Authors: Ichio Shimada; Takumi Ueda; Yutaka Kofuku; Matthew T Eddy; Kurt Wüthrich
Journal: Nat Rev Drug Discov Date: 2018-11-09 Impact factor: 84.694

Review 3. Solution NMR: A powerful tool for structural and functional studies of membrane proteins in reconstituted environments.

Authors: Robbins Puthenveetil; Olga Vinogradova
Journal: J Biol Chem Date: 2019-09-24 Impact factor: 5.157

4. Solution NMR spectroscopy of GPCRs: Residue-specific labeling strategies with a focus on ¹³C-methyl methionine labeling of the atypical chemokine receptor ACKR3.

Authors: Andrew B Kleist; Francis Peterson; Robert C Tyler; Martin Gustavsson; Tracy M Handel; Brian F Volkman
Journal: Methods Cell Biol Date: 2018-11-15 Impact factor: 1.441