| Literature DB >> 29077445 |
Ruslan P Kurta1, Jeffrey J Donatelli2,3, Chun Hong Yoon4, Peter Berntsen5, Johan Bielecki1,6, Benedikt J Daurer6, Hasan DeMirci7,8, Petra Fromme9, Max Felix Hantke6, Filipe R N C Maia6,10, Anna Munke6, Carl Nettelblad6,11, Kanupriya Pande3,12, Hemanth K N Reddy6, Jonas A Sellberg6,13, Raymond G Sierra4, Martin Svenda6, Gijs van der Schot6, Ivan A Vartanyants14,15, Garth J Williams16, P Lourdu Xavier17,18, Andrew Aquila4, Peter H Zwart3,12, Adrian P Mancuso1.
Abstract
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.Entities:
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Year: 2017 PMID： 29077445 PMCID： PMC5757528 DOI： 10.1103/PhysRevLett.119.158102
Source DB: PubMed Journal: Phys Rev Lett ISSN： 0031-9007 Impact factor: 9.161