| Literature DB >> 28869609 |
Raghu R Edupuganti1, Simon Geiger2, Rik G H Lindeboom1, Hailing Shi3, Phillip J Hsu3, Zhike Lu3, Shuang-Yin Wang1, Marijke P A Baltissen1, Pascal W T C Jansen1, Martin Rossa2, Markus Müller2, Hendrik G Stunnenberg1, Chuan He3, Thomas Carell2, Michiel Vermeulen1.
Abstract
RNA modifications are integral to the regulation of RNA metabolism. One abundant mRNA modification is N6-methyladenosine (m6A), which affects various aspects of RNA metabolism, including splicing, translation and degradation. Current knowledge about the proteins recruited to m6A to carry out these molecular processes is still limited. Here we describe comprehensive and systematic mass-spectrometry-based screening of m6A interactors in various cell types and sequence contexts. Among the main findings, we identified G3BP1 as a protein that is repelled by m6A and positively regulates mRNA stability in an m6A-regulated manner. Furthermore, we identified FMR1 as a sequence-context-dependent m6A reader, thus revealing a connection between an mRNA modification and an autism spectrum disorder. Collectively, our data represent a rich resource and shed further light on the complex interplay among m6A, m6A interactors and mRNA homeostasis.Entities:
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Year: 2017 PMID： 28869609 PMCID： PMC5725193 DOI： 10.1038/nsmb.3462
Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369