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Literature DB >> 18453706

A preliminary neutron crystallographic study of thaumatin.

Susana C M Teixeira¹, Matthew P Blakeley, Ricardo M F Leal, Edward P Mitchell, V Trevor Forsyth.

Abstract

A preliminary neutron crystallographic study of the sweet protein thaumatin is presented. Large hydrogenated crystals were prepared in deuterated crystallization buffer using the gel-acupuncture method. Data were collected to a resolution of 2 A on the LADI-III diffractometer at the Institut Laue Langevin (ILL). The results demonstrate the feasibility of a full neutron crystallographic analysis of this structure aimed at providing relevant information on the location of H atoms, the distribution of charge on the protein surface and localized water in the structure. This information will be of interest for understanding the specificity of thaumatin-receptor interactions and will contribute to further understanding of the molecular mechanisms underlying the perception of taste.

Entities: Chemical Disease

Mesh：

Substances：

Year: 2008 PMID： 18453706 PMCID： PMC2376403 DOI： 10.1107/S1744309108008294

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

36 in total

1. Surfactant layers at the air/water interface: structure and composition.

Authors: J R Lu; R K Thomas; J Penfold
Journal: Adv Colloid Interface Sci Date: 2000-01-01 Impact factor: 12.984

2. Comparison of hydrogen determination with X-ray and neutron crystallography in a human aldose reductase-inhibitor complex.

Authors: M P Blakeley; A Mitschler; I Hazemann; F Meilleur; D A A Myles; A Podjarny
Journal: Eur Biophys J Date: 2006-04-19 Impact factor: 1.733

3. Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatin.

Authors: Teodorico Tancredi; Annalisa Pastore; Severo Salvadori; Veronica Esposito; Piero A Temussi
Journal: Eur J Biochem Date: 2004-06

4. Molecular genetic identification of a candidate receptor gene for sweet taste.

Authors: M Kitagawa; Y Kusakabe; H Miura; Y Ninomiya; A Hino
Journal: Biochem Biophys Res Commun Date: 2001-04-27 Impact factor: 3.575

5. A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin.

Authors: Monika Budayova-Spano; Françoise Bonneté; Natalie Ferté; Mohamed El Hajji; Flora Meilleur; Matthew Paul Blakeley; Bertrand Castro
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2006-02-28

6. Quantum model of catalysis based on a mobile proton revealed by subatomic x-ray and neutron diffraction studies of h-aldose reductase.

Authors: Matthew P Blakeley; Federico Ruiz; Raul Cachau; Isabelle Hazemann; Flora Meilleur; Andre Mitschler; Stephan Ginell; Pavel Afonine; Oscar N Ventura; Alexandra Cousido-Siah; Michael Haertlein; Andrzej Joachimiak; Dean Myles; Alberto Podjarny
Journal: Proc Natl Acad Sci U S A Date: 2008-02-04 Impact factor: 11.205

7. In situ data collection and structure refinement from microcapillary protein crystallization.

Authors: Maneesh K Yadav; Cory J Gerdts; Ruslan Sanishvili; Ward W Smith; L Spencer Roach; Rustem F Ismagilov; Peter Kuhn; Raymond C Stevens
Journal: J Appl Crystallogr Date: 2005-12 Impact factor: 3.304

8. Identification and characterization of a fruit-specific, thaumatin-like protein that accumulates at very high levels in conjunction with the onset of sugar accumulation and berry softening in grapes.

Authors: D B Tattersall; R van Heeswijck; P B Høj
Journal: Plant Physiol Date: 1997-07 Impact factor: 8.340

A preliminary neutron crystallographic study of thaumatin.

1. Surfactant layers at the air/water interface: structure and composition.

2. Comparison of hydrogen determination with X-ray and neutron crystallography in a human aldose reductase-inhibitor complex.

3. Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatin.

4. Molecular genetic identification of a candidate receptor gene for sweet taste.

5. A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin.

6. Quantum model of catalysis based on a mobile proton revealed by subatomic x-ray and neutron diffraction studies of h-aldose reductase.

7. In situ data collection and structure refinement from microcapillary protein crystallization.

8. Identification and characterization of a fruit-specific, thaumatin-like protein that accumulates at very high levels in conjunction with the onset of sugar accumulation and berry softening in grapes.

9. Mammalian sweet taste receptors.

Review 10. Natural sweet macromolecules: how sweet proteins work.

1. High-resolution structure of the recombinant sweet-tasting protein thaumatin I.

2. A preliminary neutron crystallographic study of an A-DNA crystal.

3. A preliminary neutron diffraction study of gamma-chymotrypsin.

4. Neutron fibre diffraction studies of amyloid using H2O/D2O isotopic replacement.

5. Preliminary neutron crystallographic study of human transthyretin.

Review 6. Neutron crystallography: opportunities, challenges, and limitations.