Literature DB >> 19255494

A preliminary neutron diffraction study of gamma-chymotrypsin.

Walter R P Novak1, Aaron G Moulin, Matthew P Blakeley, Ilme Schlichting, Gregory A Petsko, Dagmar Ringe.   

Abstract

The crystal preparation and preliminary neutron diffraction analysis of gamma-chymotrypsin are presented. Large hydrogenated crystals of gamma-chymotrypsin were exchanged into deuterated buffer via vapor diffusion in a capillary and neutron Laue diffraction data were collected from the resulting crystal to 2.0 A resolution on the LADI-III diffractometer at the Institut Laue-Langevin (ILL) at room temperature. The neutron structure of a well studied protein such as gamma-chymotrypsin, which is also amenable to ultrahigh-resolution X-ray crystallography, represents the first step in developing a model system for the study of H atoms in protein crystals.

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Year:  2009        PMID： 19255494      PMCID： PMC2650460          DOI： 10.1107/S1744309109006630

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  31 in total

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Journal:  Proteins       Date:  1996-12

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Authors:  M M Dixon; B W Matthews
Journal:  Biochemistry       Date:  1989-08-22       Impact factor: 3.162

7.  Preliminary neutron diffraction studies of Escherichia coli dihydrofolate reductase bound to the anticancer drug methotrexate.

Authors:  Brad C Bennett; Flora Meilleur; Dean A A Myles; Elizabeth E Howell; Chris G Dealwis
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-04-20

8.  Nuclear magnetic resonance and neutron diffraction studies of the complex of ribonuclease A with uridine vanadate, a transition-state analogue.

Authors:  B Borah; C W Chen; W Egan; M Miller; A Wlodawer; J S Cohen
Journal:  Biochemistry       Date:  1985-04-09       Impact factor: 3.162

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Authors:  X D Cheng; B P Schoenborn
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10.  Inhibition of chymotrypsin by a complex of ortho-vanadate and benzohydroxamic acid: structure of the inert complex and its mechanistic interpretation.

Authors:  Aaron Moulin; Jason H Bell; R F Pratt; Dagmar Ringe
Journal:  Biochemistry       Date:  2007-05-01       Impact factor: 3.162
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  1 in total

1.  Time-of-flight neutron diffraction study of bovine γ-chymotrypsin at the Protein Crystallography Station.

Authors:  Louis M Lazar; S Zoe Fisher; Aaron G Moulin; Andrey Kovalevsky; Walter R P Novak; Paul Langan; Gregory A Petsko; Dagmar Ringe
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-04-27
  1 in total

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