Literature DB >> 6095205

Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

J E Marugg, N Piel, L W McLaughlin, M Tromp, G H Veeneman, G A van der Marel, J H van Boom.   

Abstract

Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1,1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5'-phosphorylated DNA-fragments on the same type of solid support.

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Year:  1984        PMID： 6095205      PMCID： PMC320404          DOI： 10.1093/nar/12.22.8639

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.

Authors:  T Dörper; E L Winnacker
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971

2.  New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

Authors:  V A Efimov; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

3.  Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

Authors:  V Kohli; A Balland; M Wintzerith; R Sauerwald; A Staub; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

4.  Synthesis of ppTppp via phosphotriester intermediates.

Authors:  C Schattenkerk; G M Visser; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1983-11-11       Impact factor: 16.971

5.  A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

Authors:  G A van der Marel; C A van Boeckel; G Wille; J H van Boom
Journal:  Nucleic Acids Res       Date:  1982-04-10       Impact factor: 16.971

6.  Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.

Authors:  M J Gait; H W Matthes; M Singh; B S Sproat; R C Titmas
Journal:  Nucleic Acids Res       Date:  1982-10-25       Impact factor: 16.971

7.  Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors:  C B Reese; L Zard
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

8.  Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.

Authors:  C T Wreesmann; A Fidder; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971
  8 in total
  11 in total

1.  RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.

Authors:  T C White; P Borst
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

2.  Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Authors:  E de Vroom; A Fidder; J E Marugg; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

3.  A general method for the synthesis of 3'-sulfhydryl and phosphate group containing oligonucleotides.

Authors:  K C Gupta; P Sharma; P Kumar; S Sathyanarayana
Journal:  Nucleic Acids Res       Date:  1991-06-11       Impact factor: 16.971

4.  The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides.

Authors:  A W Cornelissen; M P Verspieren; J J Toulmé; B W Swinkels; P Borst
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

5.  Synthesis of nucleic acid methylphosphonates via the 1-hydroxybenzotriazole phosphotriester approach.

Authors:  J E Marugg; E de Vroom; C E Dreef; M Tromp; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

6.  Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco.

Authors:  R A Hooft van Huijsduijnen; B J Cornelissen; L C van Loon; J H van Boom; M Tromp; J F Bol
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598

7.  Topogenesis of microbody enzymes: a sequence comparison of the genes for the glycosomal (microbody) and cytosolic phosphoglycerate kinases of Trypanosoma brucei.

Authors:  K A Osinga; B W Swinkels; W C Gibson; P Borst; G H Veeneman; J H Van Boom; P A Michels; F R Opperdoes
Journal:  EMBO J       Date:  1985-12-30       Impact factor: 11.598

8.  Two tandemly linked identical genes code for the glycosomal glyceraldehyde-phosphate dehydrogenase in Trypanosoma brucei.

Authors:  P A Michels; A Poliszczak; K A Osinga; O Misset; J Van Beeumen; R K Wierenga; P Borst; F R Opperdoes
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

9.  Characterization of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei.

Authors:  B W Swinkels; W C Gibson; K A Osinga; R Kramer; G H Veeneman; J H van Boom; P Borst
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598

10.  The topogenic signal of the glycosomal (microbody) phosphoglycerate kinase of Crithidia fasciculata resides in a carboxy-terminal extension.

Authors:  B W Swinkels; R Evers; P Borst
Journal:  EMBO J       Date:  1988-04       Impact factor: 11.598
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