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Literature DB >> 7088712

A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

G A van der Marel, C A van Boeckel, G Wille, J H van Boom.

Abstract

A new and attractive phosphorylation procedure which allows the introduction, via phosphotriester intermediates, of 5'-phosphate functions of DNA fragments is described. The method is based on the activation of bifunctional phosphorylating agents with 1-hydroxybenzotriazole. The approach will be exemplified by the synthesis of pACGC using four different 5'-phosphotriester intermediates.

Entities: Chemical

Mesh：

Substances：

Year: 1982 PMID： 7088712 PMCID： PMC320613 DOI： 10.1093/nar/10.7.2337

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

1 in total

1. Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.

Authors: J H van Boom; J F de Rooy
Journal: J Chromatogr Date: 1977-01-21

1 in total

10 in total

1. Conformational and thermodynamic consequences of the introduction of a nick in duplexed DNA fragments: an NMR study augmented by biochemical experiments.

Authors: J M Pieters; R M Mans; H van den Elst; G A van der Marel; J H van Boom; C Altona
Journal: Nucleic Acids Res Date: 1989-06-26 Impact factor: 16.971

2. Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Authors: J E Marugg; N Piel; L W McLaughlin; M Tromp; G H Veeneman; G A van der Marel; J H van Boom
Journal: Nucleic Acids Res Date: 1984-11-26 Impact factor: 16.971

3. Preparation of oligonucleotides corresponding to the acceptor stem of yeast tRNAPhe and their interaction with yeast ATP(CTP):tRNA nucleotidyltransferase.

Authors: G H Wang; L W McLaughlin; H Sternbach; F Cramer
Journal: Nucleic Acids Res Date: 1984-09-11 Impact factor: 16.971

4. Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Authors: T Kempe; W I Sundquist; F Chow; S L Hu
Journal: Nucleic Acids Res Date: 1985-01-11 Impact factor: 16.971

5. Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support.

Authors: T Tanaka; T Sakata; K Fujimoto; M Ikehara
Journal: Nucleic Acids Res Date: 1987-08-11 Impact factor: 16.971

10. Preparation of the individual diastereomers of adenylyl-(2'-5')-P-thioadenylyl-(2'-5')-adenosine and their 5'-phosphorylated derivatives.

Authors: E de Vroom; A Fidder; C P Saris; G A van der Marel; J H van Boom
Journal: Nucleic Acids Res Date: 1987-12-10 Impact factor: 16.971

10 in total

A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

1. Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.

1. Conformational and thermodynamic consequences of the introduction of a nick in duplexed DNA fragments: an NMR study augmented by biochemical experiments.

2. Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

3. Preparation of oligonucleotides corresponding to the acceptor stem of yeast tRNAPhe and their interaction with yeast ATP(CTP):tRNA nucleotidyltransferase.

4. Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

5. Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support.

6. Synthesis of riboguanosine pentaphosphate ppprGpp (Magic Spot II) via a phosphotriester approach.

7. Synthesis of ppTppp via phosphotriester intermediates.

8. Conformational analysis of oligoarabinonucleotides. An NMR and CD study.

9. Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.

10. Preparation of the individual diastereomers of adenylyl-(2'-5')-P-thioadenylyl-(2'-5')-adenosine and their 5'-phosphorylated derivatives.