Literature DB >> 6296777

Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

V Kohli, A Balland, M Wintzerith, R Sauerwald, A Staub, J P Lecocq.   

Abstract

The phosphotriester method for the stepwise synthesis of deoxyoligonucleotides has been employed using HPLC-grade silica gel (Porasil B) as the solid support. The procedure results in a convenient flow-through system for the synthesis of oligomers where all the reaction steps including the zinc bromide method of detritylation are compatible with the selected support. Deoxyoligonucleotides of 25-30 nucleotides in length can be synthesized in high yields utilising stable phosphotriester intermediates. Ease of handling of the solid support allows convenient synthesis of mixed oligonucleotide sequences.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6296777      PMCID： PMC327016          DOI： 10.1093/nar/10.22.7439

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  A general method for inserting specific DNA sequences into cloning vehicles.

Authors:  C P Bahl; K J Marians; R Wu
Journal:  Gene       Date:  1976       Impact factor: 3.688

2.  Total synthesis of a human leukocyte interferon gene.

Authors:  M D Edge; A R Green; G R Heathcliffe; P A Meacock; W Schuch; D B Scanlon; T C Atkinson; C R Newton; A F Markham
Journal:  Nature       Date:  1981-08-20       Impact factor: 49.962

3.  Synthesis of oligodeoxyribonucleotides on silica gel support.

Authors:  F Chow; T Kempe; G Palm
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

4.  Sequence analysis of short DNA fragments.

Authors:  C P Tu; R Wu
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Chemical synthesis of genes for human insulin.

Authors:  R Crea; A Kraszewski; T Hirose; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

7.  Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

8.  High-pressure liquid chromatography in polynucleotide synthesis.

Authors:  H J Fritz; R Belagaje; E L Brown; R H Fritz; R A Jones; R G Lees; H G Khorana
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

9.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728

10.  Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Miyake; T Hozumi; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
View more
  7 in total

1.  Mass spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments.

Authors:  L Alder; A Rosenthal; D Cech
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

2.  Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Authors:  J E Marugg; N Piel; L W McLaughlin; M Tromp; G H Veeneman; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

3.  High-level production of biologically active human alpha 1-antitrypsin in Escherichia coli.

Authors:  M Courtney; A Buchwalder; L H Tessier; M Jaye; A Benavente; A Balland; V Kohli; R Lathe; P Tolstoshev; J P Lecocq
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

4.  Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.

Authors:  M Jaye; H de la Salle; F Schamber; A Balland; V Kohli; A Findeli; P Tolstoshev; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1983-04-25       Impact factor: 16.971

5.  The influence of mRNA primary and secondary structure on human IFN-gamma gene expression in E. coli.

Authors:  L H Tessier; P Sondermeyer; T Faure; D Dreyer; A Benavente; D Villeval; M Courtney; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1984-10-25       Impact factor: 16.971

6.  Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis.

Authors:  R P Harvey; E Degryse; L Stefani; F Schamber; J P Cazenave; M Courtney; P Tolstoshev; J P Lecocq
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.

Authors:  H W Matthes; W M Zenke; T Grundström; A Staub; M Wintzerith; P Chambon
Journal:  EMBO J       Date:  1984-04       Impact factor: 11.598
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.