Literature DB >> 7177846

Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.

M J Gait, H W Matthes, M Singh, B S Sproat, R C Titmas.   

Abstract

A new kieselguhr-polydimethylacrylamide support has been used in a continuous flow, column system for solid phase synthesis of oligodeoxyribonucleotides by a phosphotriester procedure. Using only protected mononucleotides a 14-mer, 20-mer and 27-mer were assembled in high repetitive yield using a simple manually operated, bench top apparatus.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 7177846      PMCID： PMC326914          DOI： 10.1093/nar/10.20.6243

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Synthesis of oligodeoxyribonucleotides on silica gel support.

Authors:  F Chow; T Kempe; G Palm
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

2.  Syringe method for stepwise chemical synthesis of oligonucleotides.

Authors:  T Tanaka; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1982-05-25       Impact factor: 16.971

3.  Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

Authors:  M J Gait; M Singh; R C Sheppard; M D Edge; A R Greene; G R Heathcliffe; T C Atkinson; C R Newton; A F Markham
Journal:  Nucleic Acids Res       Date:  1980-03-11       Impact factor: 16.971

4.  Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T).

Authors:  M J Gait; R C Sheppard
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

5.  Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method.

Authors:  K Miyoshi; K Itakura
Journal:  Nucleic Acids Symp Ser       Date:  1980

6.  Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.

Authors:  K E Norris; F Norris; K Brunfeldt
Journal:  Nucleic Acids Symp Ser       Date:  1980

7.  Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

Authors:  H Ito; Y Ike; S Ikuta; K Itakura
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

8.  Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

9.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971

10.  Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).

Authors:  T Horn; M P Vasser; M E Struble; R Crea
Journal:  Nucleic Acids Symp Ser       Date:  1980
  10 in total
  35 in total

1.  Prediction of oligodeoxyribonucleotides eluting salt conditions on anion exchange column.

Authors:  J L Vaerman; P Moureau; Y Larondelle; P Martiat; M Philippe
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

2.  Ribulose 1,5-bisphosphate carboxylase. Effect on the catalytic properties of changing methionine-330 to leucine in the Rhodospirillum rubrum enzyme.

Authors:  B E Terzaghi; W A Laing; J T Christeller; G B Petersen; D F Hill
Journal:  Biochem J       Date:  1986-05-01       Impact factor: 3.857

3.  EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.

Authors:  M M Waye; M E Verhoeyen; P T Jones; G Winter
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971

4.  Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms.

Authors:  C J Herbert; M Sarwar; S S Ner; I G Giles; M Akhtar
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857

5.  Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs.

Authors:  W B Cruse; J Aymani; O Kennard; T Brown; A G Jack; G A Leonard
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

6.  Improved synthesis of oligodeoxyribonucleotide using 3-methoxy-4-phenoxybenzoyl group for amino protection.

Authors:  R K Mishra; K Misra
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

7.  Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment.

Authors:  T Brown; G Kneale; W N Hunter; O Kennard
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

8.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

9.  Solid-phase synthesis of DNA fragments containing the modified base 7-hydro-8-oxo-2'-deoxyguanosine.

Authors:  H C Roelen; C P Saris; H F Brugghe; H van den Elst; J G Westra; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

10.  Inosine.adenine base pairs in a B-DNA duplex.

Authors:  P W Corfield; W N Hunter; T Brown; P Robinson; O Kennard
Journal:  Nucleic Acids Res       Date:  1987-10-12       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.