Literature DB >> 7177853

New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

V A Efimov, S V Reverdatto, O G Chakhmakhcheva.   

Abstract

A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucleotides as coupling units.

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Year:  1982        PMID： 7177853      PMCID： PMC326957          DOI： 10.1093/nar/10.21.6675

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  The use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach.

Authors:  N Katagiri; K Itakura; S A Narang
Journal:  J Am Chem Soc       Date:  1975-12-10       Impact factor: 15.419

2.  A reliable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysis.

Authors:  C P Tu; E Jay; C P Bahl; R Wu
Journal:  Anal Biochem       Date:  1976-07       Impact factor: 3.365

3.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

4.  Chemical synthesis of deoxyoligonucleotides by the modified triester method.

Authors:  S A Narang; R Brousseau; H M Hsiung; J J Michniewicz
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

5.  Chemical synthesis of genes for human insulin.

Authors:  R Crea; A Kraszewski; T Hirose; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

6.  Protected deoxyribonucleoside-3' aryl phosphodiesters as key intermediates in polynucleotide synthesis. Construction of an icosanucleotide analogous to the sequence at the ends of Rous sarcoma virus 35S RNA.

Authors:  G R Gough; C K Singleton; H L Weith; P T Gilham
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

7.  High-pressure liquid chromatography in polynucleotide synthesis.

Authors:  H J Fritz; R Belagaje; E L Brown; R H Fritz; R A Jones; R G Lees; H G Khorana
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

8.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

9.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

10.  A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides.

Authors:  A K Seth; E Jay
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
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  28 in total

1.  Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms.

Authors:  C J Herbert; M Sarwar; S S Ner; I G Giles; M Akhtar
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857

2.  Thermodynamic behaviour of the heptadecadeoxynucleotide d(CGCGCGTTTTTCGCGCG) forming B and Z hairpins in aqueous solution.

Authors:  L E Xodo; G Manzini; F Quadrifoglio; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

3.  Synthesis of DNA fragments containing 5,6-dihydrothymine, a major product of thymine gamma radiolysis.

Authors:  J C Schulhof; D Molko; R Teoule
Journal:  Nucleic Acids Res       Date:  1988-01-11       Impact factor: 16.971

4.  Oligonucleotides, part 5+: synthesis and fluorescence studies of DNA oligomers d(AT)5 containing adenines covalently linked at C-8 with dansyl fluorophore.

Authors:  D Singh; V Kumar; K N Ganesh
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

5.  HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.

Authors:  G M Bonora; C L Scremin; F P Colonna; A Garbesi
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

6.  General scheme of the phosphotriester condensation in the oligodeoxyribonucleotide synthesis with arylsulfonyl chlorides and arylsulfonyl azolides.

Authors:  V F Zarytova; D G Knorre
Journal:  Nucleic Acids Res       Date:  1984-02-24       Impact factor: 16.971

7.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

8.  Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach.

Authors:  G M Bonora; G Biancotto; M Maffini; C L Scremin
Journal:  Nucleic Acids Res       Date:  1993-03-11       Impact factor: 16.971

9.  Guanine modification during chemical DNA synthesis.

Authors:  J S Eadie; D S Davidson
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

10.  Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis.

Authors:  V A Efimov; A A Buryakova; I Y Dubey; N N Polushin; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1986-08-26       Impact factor: 16.971
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