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Literature DB >> 6856470

Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides.

Abstract

The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reactions during solid-phase synthesis can thus be performed with high efficiency and result in a high yield synthesis of long chain oligodeoxyribonucleotides.

Entities: Chemical

Mesh：

Substances：

Year: 1983 PMID： 6856470 PMCID： PMC325909 DOI： 10.1093/nar/11.9.2575

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

4 in total

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Authors: R L Letsinger; W B Lunsford
Journal: J Am Chem Soc Date: 1976-06-09 Impact factor: 15.419

2. Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates.

Authors: G W Daub; E E van Tamelen
Journal: J Am Chem Soc Date: 1977-05-11 Impact factor: 15.419

3. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

4. Gene expression: chemical synthesis of E. coli ribosome binding sites and their use in directing the expression of mammalian proteins in bacteria.

Authors: E Jay; A K Seth; J Rommens; A Sood; G Jay
Journal: Nucleic Acids Res Date: 1982-10-25 Impact factor: 16.971

4 in total

20 in total

1. Mutational analysis of the immunoglobulin heavy chain promoter region.

Authors: D W Ballard; A Bothwell
Journal: Proc Natl Acad Sci U S A Date: 1986-12 Impact factor: 11.205

2. Generation of a variant t(2;8) translocation of Burkitt's lymphoma by site-specific recombination via the kappa light-chain joining signals.

Authors: P Hartl; M Lipp
Journal: Mol Cell Biol Date: 1987-06 Impact factor: 4.272

3. Synthesis and application of derivatizable oligonucleotides.

Authors: K J Gibson; S J Benkovic
Journal: Nucleic Acids Res Date: 1987-08-25 Impact factor: 16.971

4. The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli.

Authors: M Brombach; C L Pon
Journal: Mol Gen Genet Date: 1987-06

5. Filter disc supported oligonucleotide synthesis by the phosphite method.

Authors: J Ott; F Eckstein
Journal: Nucleic Acids Res Date: 1984-12-11 Impact factor: 16.971

6. Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Authors: J E Marugg; N Piel; L W McLaughlin; M Tromp; G H Veeneman; G A van der Marel; J H van Boom
Journal: Nucleic Acids Res Date: 1984-11-26 Impact factor: 16.971

7. Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides.

Authors: J Nielsen; M Taagaard; J E Marugg; J H van Boom; O Dahl
Journal: Nucleic Acids Res Date: 1986-09-25 Impact factor: 16.971

8. Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.

Authors: B A Connolly; P Rider
Journal: Nucleic Acids Res Date: 1985-06-25 Impact factor: 16.971

9. Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.

Authors: T Tanaka; S Tamatsukuri; M Ikehara
Journal: Nucleic Acids Res Date: 1986-08-11 Impact factor: 16.971

10. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.

Authors: R Schneider; I Gander; U Müller; R Mertz; E L Winnacker
Journal: Nucleic Acids Res Date: 1986-02-11 Impact factor: 16.971