Literature DB >> 6200828

Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.

C T Wreesmann, A Fidder, G A van der Marel, J H van Boom.   

Abstract

Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.

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Year:  1983        PMID： 6200828      PMCID： PMC326589          DOI： 10.1093/nar/11.23.8389

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  The case of sulfonation in the chemical synthesis of oligonucleotides.

Authors:  A Kraszewski; J Stawiński; M Wiewiórowski
Journal:  Nucleic Acids Res       Date:  1980-05-24       Impact factor: 16.971

2.  The use of high-performance liquid chromatography in the isolation and analysis of oligoribonucleotides synthesized by the T4 RNA ligase reaction.

Authors:  L W McLaughlin; E Romaniuk
Journal:  Anal Biochem       Date:  1982-07-15       Impact factor: 3.365

3.  Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.

Authors:  J H van Boom; P M Burgers; G van der Marel; C H Verdegaal; G Wille
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

4.  Further studies on oligoribonucleotide synthesis.

Authors:  W T Markiewicz; E Biała; R W Adamiak; K Grześkowiak; R Kierzek; A Kraszewski; J Stawiński; M Wiewiórowski
Journal:  Nucleic Acids Symp Ser       Date:  1980

5.  High-pressure liquid chromatography in polynucleotide synthesis.

Authors:  H J Fritz; R Belagaje; E L Brown; R H Fritz; R A Jones; R G Lees; H G Khorana
Journal:  Biochemistry       Date:  1978-04-04       Impact factor: 3.162

6.  Conformational analysis of the single-stranded ribonucleic acid A-A-C-C. A one-dimensional and two-dimensional proton NMR study at 500 MHz.

Authors:  J Doornbos; C T Wreesmann; J H Van Boom; C Altona
Journal:  Eur J Biochem       Date:  1983-04-05

7.  A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides.

Authors:  A K Seth; E Jay
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
  7 in total
  6 in total

1.  Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Authors:  E de Vroom; A Fidder; J E Marugg; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

2.  Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.

Authors:  J E Marugg; N Piel; L W McLaughlin; M Tromp; G H Veeneman; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

3.  Preparation of oligonucleotides corresponding to the acceptor stem of yeast tRNAPhe and their interaction with yeast ATP(CTP):tRNA nucleotidyltransferase.

Authors:  G H Wang; L W McLaughlin; H Sternbach; F Cramer
Journal:  Nucleic Acids Res       Date:  1984-09-11       Impact factor: 16.971

4.  Preparation of covalently linked DNA-RNA hybrids and arabinocytidine containing DNA fragments.

Authors:  E de Vroom; H C Roelen; C P Saris; T N Budding; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1988-04-11       Impact factor: 16.971

5.  Synthesis of nucleic acid methylphosphonates via the 1-hydroxybenzotriazole phosphotriester approach.

Authors:  J E Marugg; E de Vroom; C E Dreef; M Tromp; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

6.  Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.

Authors:  E de Vroom; H J Broxterman; L A Sliedregt; G A van der Marel; J H van Boom
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971
  6 in total

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