| Literature DB >> 26919431 |
Natsuko Izumi1, Keisuke Shoji2, Yuriko Sakaguchi3, Shozo Honda4, Yohei Kirino4, Tsutomu Suzuki3, Susumu Katsuma2, Yukihide Tomari5.
Abstract
PIWI-interacting RNAs (piRNAs) play a crucial role in transposon silencing in animal germ cells. In piRNA biogenesis, single-stranded piRNA intermediates are loaded into PIWI-clade proteins and cleaved by Zucchini/MitoPLD, yielding precursor piRNAs (pre-piRNAs). Pre-piRNAs that are longer than the mature piRNA length are then trimmed at their 3' ends. Although recent studies implicated the Tudor domain protein Papi/Tdrkh in pre-piRNA trimming, the identity of Trimmer and its relationship with Papi/Tdrkh remain unknown. Here, we identified PNLDC1, an uncharacterized 3'-5' exonuclease, as Trimmer in silkworms. Trimmer is enriched in the mitochondrial fraction and binds to Papi/Tdrkh. Depletion of Trimmer and Papi/Tdrkh additively inhibits trimming, causing accumulation of ∼35-40-nt pre-piRNAs that are impaired for target cleavage and prone to degradation. Our results highlight the cooperative action of Trimmer and Papi/Tdrkh in piRNA maturation.Entities:
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Year: 2016 PMID： 26919431 PMCID： PMC4856147 DOI： 10.1016/j.cell.2016.01.008
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582