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Literature DB >> 17384646

The 3' termini of mouse Piwi-interacting RNAs are 2'-O-methylated.

Tomoya Ohara¹, Yuriko Sakaguchi, Takeo Suzuki, Hiroki Ueda, Kenjyo Miyauchi, Tsutomu Suzuki.

Abstract

Piwi-interacting RNAs (piRNAs) are a germline-specific class of small noncoding RNAs that are essential for spermatogenesis, but their function and biogenesis remain elusive. Here we report a post-transcriptional modification of mouse piRNAs. Mass spectrometric analysis reveals that the piRNAs tested are fully modified by 2'-O-methylation at their 3' termini. This observation may provide a clue to the biogenesis and function of piRNAs in spermatogenesis.

Entities: Gene Species

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Year: 2007 PMID： 17384646 DOI： 10.1038/nsmb1220

Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369

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Cited

113 in total

1. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA.

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Journal: Cell Res Date: 2011-09-20 Impact factor: 25.617

2. A role for transcription from a piRNA cluster in de novo piRNA production.

Authors: Shinpei Kawaoka; Hiroshi Mitsutake; Takashi Kiuchi; Maki Kobayashi; Mayu Yoshikawa; Yutaka Suzuki; Sumio Sugano; Toru Shimada; Jun Kobayashi; Yukihide Tomari; Susumu Katsuma
Journal: RNA Date: 2011-12-22 Impact factor: 4.942

3. Profiling sex-specific piRNAs in zebrafish.

Authors: Xiang Zhou; Zhixiang Zuo; Fang Zhou; Wei Zhao; Yuriko Sakaguchi; Takeo Suzuki; Tsutomu Suzuki; Hanhua Cheng; Rongjia Zhou
Journal: Genetics Date: 2010-09-13 Impact factor: 4.562

4. Kinetic and functional analysis of the small RNA methyltransferase HEN1: the catalytic domain is essential for preferential modification of duplex RNA.

Authors: Giedrius Vilkaitis; Alexandra Plotnikova; Saulius Klimasauskas
Journal: RNA Date: 2010-08-12 Impact factor: 4.942

The 3' termini of mouse Piwi-interacting RNAs are 2'-O-methylated.

1. Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA.

2. A role for transcription from a piRNA cluster in de novo piRNA production.

3. Profiling sex-specific piRNAs in zebrafish.

4. Kinetic and functional analysis of the small RNA methyltransferase HEN1: the catalytic domain is essential for preferential modification of duplex RNA.

5. miR-ID: a novel, circularization-based platform for detection of microRNAs.

6. Selective amplification and sequencing of cyclic phosphate-containing RNAs by the cP-RNA-seq method.

7. Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity.

8. A marked end.

9. The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians.

10. piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism.