| Literature DB >> 25477465 |
Jason Tenboer1, Shibom Basu2, Nadia Zatsepin3, Kanupriya Pande1, Despina Milathianaki4, Matthias Frank5, Mark Hunter5, Sébastien Boutet4, Garth J Williams4, Jason E Koglin4, Dominik Oberthuer6, Michael Heymann7, Christopher Kupitz2, Chelsie Conrad2, Jesse Coe2, Shatabdi Roy-Chowdhury2, Uwe Weierstall3, Daniel James3, Dingjie Wang3, Thomas Grant8, Anton Barty7, Oleksandr Yefanov7, Jennifer Scales1, Cornelius Gati6,7, Carolin Seuring6, Vukica Srajer9, Robert Henning9, Peter Schwander1, Raimund Fromme2, Abbas Ourmazd1, Keith Moffat9,10, Jasper J Van Thor11, John C H Spence3, Petra Fromme2, Henry N Chapman6,7, Marius Schmidt1,12.
Abstract
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.Entities:
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Year: 2014 PMID： 25477465 PMCID： PMC4361027 DOI： 10.1126/science.1259357
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728