Nathaniel V Nucci1, Kathleen G Valentine1, A Joshua Wand2. 1. Department of Biochemistry and Biophysics, Johnson Research Foundation, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104-6059, USA. 2. Department of Biochemistry and Biophysics, Johnson Research Foundation, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104-6059, USA. Electronic address: wand@mail.med.upenn.edu.
Abstract
High-resolution multi-dimensional solution NMR is unique as a biophysical and biochemical tool in its ability to examine both the structure and dynamics of macromolecules at atomic resolution. Conventional solution NMR approaches, however, are largely limited to examinations of relatively small (<25kDa) molecules, mostly due to the spectroscopic consequences of slow rotational diffusion. Encapsulation of macromolecules within the protective nanoscale aqueous interior of reverse micelles dissolved in low viscosity fluids has been developed as a means through which the 'slow tumbling problem' can be overcome. This approach has been successfully applied to diverse proteins and nucleic acids ranging up to 100kDa, considerably widening the range of biological macromolecules to which conventional solution NMR methodologies may be applied. Recent advances in methodology have significantly broadened the utility of this approach in structural biology and molecular biophysics.
High-resolution multi-dimensional solution NMR is unique as a biophysical and biochemical tool in its ability to examine both the structure and dynamics of macromolecules at atomic resolution. Conventional solutionn class="Chemical">NMR approaches, however, are largely limited to examinations of relatively small (<25kDa) molecules, mostly due to the spectroscopic consequences of slow rotational diffusion. Encapsulation of macromolecules within the protective nanoscale aqueous interior of reverse micelles dissolved in low viscosity fluids has been developed as a means through which the 'slow tumbling problem' can be overcome. This approach has been successfully applied to diverse proteins and nucleic acids ranging up to 100kDa, considerably widening the range of biological macromolecules to which conventional solution NMR methodologies may be applied. Recent advances in methodology have significantly broadened the utility of this approach in structural biology and molecular biophysics.
Authors: Nathaniel V Nucci; Bryan S Marques; Sabrina Bédard; Jakob Dogan; John M Gledhill; Veronica R Moorman; Ronald W Peterson; Kathleen G Valentine; Alison L Wand; A Joshua Wand Journal: J Biomol NMR Date: 2011-07-12 Impact factor: 2.835
Authors: Kathleen G Valentine; Ronald W Peterson; Jamil S Saad; Michael F Summers; Xianzhong Xu; James B Ames; A Joshua Wand Journal: Structure Date: 2010-01-13 Impact factor: 5.006
Authors: Igor Dodevski; Nathaniel V Nucci; Kathleen G Valentine; Gurnimrat K Sidhu; Evan S O'Brien; Arthur Pardi; A Joshua Wand Journal: J Am Chem Soc Date: 2014-02-19 Impact factor: 15.419
Authors: José A Caro; Kyle W Harpole; Vignesh Kasinath; Jackwee Lim; Jeffrey Granja; Kathleen G Valentine; Kim A Sharp; A Joshua Wand Journal: Proc Natl Acad Sci U S A Date: 2017-06-05 Impact factor: 11.205
Authors: Nathaniel V Nucci; Brian Fuglestad; Evangelia A Athanasoula; A Joshua Wand Journal: Proc Natl Acad Sci U S A Date: 2014-09-08 Impact factor: 11.205
Authors: Brian Fuglestad; Bryan S Marques; Christine Jorge; Nicole E Kerstetter; Kathleen G Valentine; A Joshua Wand Journal: Methods Enzymol Date: 2018-12-10 Impact factor: 1.600