Literature DB >> 24264768

α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression.

Roman J Lichtenecker1, Katharina Weinhäupl, Walther Schmid, Robert Konrat.   

Abstract

(13)C-α-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing E. coli strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of (15)N-chiral amino acid synthesis using organic chemistry.

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Year:  2013        PMID： 24264768     DOI： 10.1007/s10858-013-9796-9

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  18 in total

1.  Synthesis of a 13C-methyl-group-labeled methionine precursor as a useful tool for simplifying protein structural analysis by NMR spectroscopy.

Authors:  Michael Fischer; Karin Kloiber; Johannes Häusler; Karin Ledolter; Robert Konrat; Walther Schmid
Journal:  Chembiochem       Date:  2007-04-16       Impact factor: 3.164

Review 2.  Anatomy of hot spots in protein interfaces.

Authors:  A A Bogan; K S Thorn
Journal:  J Mol Biol       Date:  1998-07-03       Impact factor: 5.469

3.  Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds.

Authors:  Roman J Lichtenecker; Nicolas Coudevylle; Robert Konrat; Walther Schmid
Journal:  Chembiochem       Date:  2013-04-05       Impact factor: 3.164

4.  NMR assignment methods for the aromatic ring resonances of phenylalanine and tyrosine residues in proteins.

Authors:  Takuya Torizawa; Akira Mei Ono; Tsutomu Terauchi; Masatsune Kainosho
Journal:  J Am Chem Soc       Date:  2005-09-14       Impact factor: 15.419

5.  A novel method for selective isotope labeling of bacterially expressed proteins.

Authors:  K M Lee; E J Androphy; J D Baleja
Journal:  J Biomol NMR       Date:  1995-01       Impact factor: 2.835

6.  Application of SAIL phenylalanine and tyrosine with alternative isotope-labeling patterns for protein structure determination.

Authors:  Mitsuhiro Takeda; Akira M Ono; Tsutomu Terauchi; Masatsune Kainosho
Journal:  J Biomol NMR       Date:  2009-08-07       Impact factor: 2.835

7.  Improved pulse sequences for sequence specific assignment of aromatic proton resonances in proteins.

Authors:  Frank Löhr; Robert Hänsel; Vladimir V Rogov; Volker Dötsch
Journal:  J Biomol NMR       Date:  2007-01-20       Impact factor: 2.835

8.  Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing.

Authors:  Roman Lichtenecker; Martin L Ludwiczek; Walther Schmid; Robert Konrat
Journal:  J Am Chem Soc       Date:  2004-05-05       Impact factor: 15.419

9.  Biosynthetic 13C labeling of aromatic side chains in proteins for NMR relaxation measurements.

Authors:  Kaare Teilum; Ulrika Brath; Patrik Lundström; Mikael Akke
Journal:  J Am Chem Soc       Date:  2006-03-01       Impact factor: 15.419

10.  A 13C labeling strategy reveals a range of aromatic side chain motion in calmodulin.

Authors:  Vignesh Kasinath; Kathleen G Valentine; A Joshua Wand
Journal:  J Am Chem Soc       Date:  2013-06-19       Impact factor: 15.419
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  17 in total

1.  Aromatic Ring Dynamics, Thermal Activation, and Transient Conformations of a 468 kDa Enzyme by Specific 1H-13C Labeling and Fast Magic-Angle Spinning NMR.

Authors:  Diego F Gauto; Pavel Macek; Alessandro Barducci; Hugo Fraga; Audrey Hessel; Tsutomu Terauchi; David Gajan; Yohei Miyanoiri; Jerome Boisbouvier; Roman Lichtenecker; Masatsune Kainosho; Paul Schanda
Journal:  J Am Chem Soc       Date:  2019-07-05       Impact factor: 15.419

2.  A sharp thermal transition of fast aromatic-ring dynamics in ubiquitin.

Authors:  Vignesh Kasinath; Yinan Fu; Kim A Sharp; A Joshua Wand
Journal:  Angew Chem Int Ed Engl       Date:  2014-12-04       Impact factor: 15.336

3.  Bacterial production of site specific 13C labeled phenylalanine and methodology for high level incorporation into bacterially expressed recombinant proteins.

Authors:  Bhargavi Ramaraju; Hana McFeeters; Bernhard Vogler; Robert L McFeeters
Journal:  J Biomol NMR       Date:  2016-12-27       Impact factor: 2.835

4.  Characterizing Fast Conformational Exchange of Aromatic Rings Using Residual Dipolar Couplings: Distinguishing Jumplike Flips from Other Exchange Mechanisms.

Authors:  Matthias Dreydoppel; Mikael Akke; Ulrich Weininger
Journal:  J Phys Chem B       Date:  2022-09-30       Impact factor: 3.466

Review 5.  Measuring Entropy in Molecular Recognition by Proteins.

Authors:  A Joshua Wand; Kim A Sharp
Journal:  Annu Rev Biophys       Date:  2018-01-18       Impact factor: 12.981

6.  Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.

Authors:  Vilius Kurauskas; Paul Schanda; Remy Sounier
Journal:  Methods Mol Biol       Date:  2017

7.  Simultaneous measurement of 1HC/N-R2's for rapid acquisition of backbone and sidechain paramagnetic relaxation enhancements (PREs) in proteins.

Authors:  C Ashley Barnes; Mary R Starich; Nico Tjandra; Pushpa Mishra
Journal:  J Biomol NMR       Date:  2021-02-24       Impact factor: 2.835

8.  Site-selective 13C labeling of proteins using erythrose.

Authors:  Ulrich Weininger
Journal:  J Biomol NMR       Date:  2017-02-28       Impact factor: 2.835

Review 9.  Late metabolic precursors for selective aromatic residue labeling.

Authors:  Julia Schörghuber; Leonhard Geist; Gerald Platzer; Michael Feichtinger; Marilena Bisaccia; Lukas Scheibelberger; Frederik Weber; Robert Konrat; Roman J Lichtenecker
Journal:  J Biomol NMR       Date:  2018-05-28       Impact factor: 2.835

10.  Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection.

Authors:  Ulrich Weininger; Ulrika Brath; Kristofer Modig; Kaare Teilum; Mikael Akke
Journal:  J Biomol NMR       Date:  2014-04-05       Impact factor: 2.835
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