| Literature DB >> 28755366 |
Vilius Kurauskas1,2,3, Paul Schanda1,2,3, Remy Sounier4.
Abstract
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.Entities:
Keywords: Detergent; Deuteration; Methyl labeling; NMR spectroscopy; Refolding
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Year: 2017 PMID： 28755366 PMCID： PMC5551963 DOI： 10.1007/978-1-4939-7151-0_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745