Literature DB >> 23564734

Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds.

Roman J Lichtenecker1, Nicolas Coudevylle, Robert Konrat, Walther Schmid.   

Abstract

You can have one without the other: A new metabolic precursor compound can selectively introduce (13)C and (2)H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2013        PMID： 23564734     DOI： 10.1002/cbic.201200737

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  15 in total

1.  Independent valine and leucine isotope labeling in Escherichia coli protein overexpression systems.

Authors:  Roman J Lichtenecker; Katharina Weinhäupl; Lukas Reuther; Julia Schörghuber; Walther Schmid; Robert Konrat
Journal:  J Biomol NMR       Date:  2013-11       Impact factor: 2.835

2.  Specific labeling and assignment strategies of valine methyl groups for NMR studies of high molecular weight proteins.

Authors:  Guillaume Mas; Elodie Crublet; Olivier Hamelin; Pierre Gans; Jérôme Boisbouvier
Journal:  J Biomol NMR       Date:  2013-11       Impact factor: 2.835

3.  α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression.

Authors:  Roman J Lichtenecker; Katharina Weinhäupl; Walther Schmid; Robert Konrat
Journal:  J Biomol NMR       Date:  2013-11-22       Impact factor: 2.835

4.  Exploiting E. coli auxotrophs for leucine, valine, and threonine specific methyl labeling of large proteins for NMR applications.

Authors:  Yoan R Monneau; Yojiro Ishida; Paolo Rossi; Tomohide Saio; Shiou-Ru Tzeng; Masayori Inouye; Charalampos G Kalodimos
Journal:  J Biomol NMR       Date:  2016-06-02       Impact factor: 2.835

5.  The guide sRNA sequence determines the activity level of box C/D RNPs.

Authors:  Andrea Graziadei; Frank Gabel; John Kirkpatrick; Teresa Carlomagno
Journal:  Elife       Date:  2020-03-23       Impact factor: 8.140

Review 6.  Measuring Entropy in Molecular Recognition by Proteins.

Authors:  A Joshua Wand; Kim A Sharp
Journal:  Annu Rev Biophys       Date:  2018-01-18       Impact factor: 12.981

Review 7.  Application and methodology of dissolution dynamic nuclear polarization in physical, chemical and biological contexts.

Authors:  Sami Jannin; Jean-Nicolas Dumez; Patrick Giraudeau; Dennis Kurzbach
Journal:  J Magn Reson       Date:  2019-06-04       Impact factor: 2.229

8.  Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.

Authors:  Vilius Kurauskas; Paul Schanda; Remy Sounier
Journal:  Methods Mol Biol       Date:  2017

9.  Structure-Based Assignment of Ile, Leu, and Val Methyl Groups in the Active and Inactive Forms of the Mitogen-Activated Protein Kinase Extracellular Signal-Regulated Kinase 2.

Authors:  Yao Xiao; Lisa R Warner; Michael P Latham; Natalie G Ahn; Arthur Pardi
Journal:  Biochemistry       Date:  2015-07-13       Impact factor: 3.162

Review 10.  Late metabolic precursors for selective aromatic residue labeling.

Authors:  Julia Schörghuber; Leonhard Geist; Gerald Platzer; Michael Feichtinger; Marilena Bisaccia; Lukas Scheibelberger; Frederik Weber; Robert Konrat; Roman J Lichtenecker
Journal:  J Biomol NMR       Date:  2018-05-28       Impact factor: 2.835
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