Literature DB >> 20383561

CACA-TOCSY with alternate 13C-12C labeling: a 13Calpha direct detection experiment for mainchain resonance assignment, dihedral angle information, and amino acid type identification.

Koh Takeuchi1, Dominique P Frueh, Zhen-Yu J Sun, Sebastian Hiller, Gerhard Wagner.   

Abstract

We present a (13)C direct detection CACA-TOCSY experiment for samples with alternate (13)C-(12)C labeling. It provides inter-residue correlations between (13)C(alpha) resonances of residue i and adjacent C(alpha)s at positions i - 1 and i + 1. Furthermore, longer mixing times yield correlations to C(alpha) nuclei separated by more than one residue. The experiment also provides C(alpha)-to-sidechain correlations, some amino acid type identifications and estimates for psi dihedral angles. The power of the experiment derives from the alternate (13)C-(12)C labeling with [1,3-(13)C] glycerol or [2-(13)C] glycerol, which allows utilizing the small scalar (3)J(CC) couplings that are masked by strong (1)J(CC) couplings in uniformly (13)C labeled samples.

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Year:  2010        PMID： 20383561      PMCID： PMC2868269          DOI： 10.1007/s10858-010-9410-3

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  23 in total

1.  Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction.

Authors:  Nobuhisa Shimba; Alan S Stern; Charles S Craik; Jeffrey C Hoch; Volker Dötsch
Journal:  J Am Chem Soc       Date:  2003-03-05       Impact factor: 15.419

2.  Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy.

Authors:  Federica Castellani; Barth van Rossum; Annette Diehl; Mario Schubert; Kristina Rehbein; Hartmut Oschkinat
Journal:  Nature       Date:  2002-11-07       Impact factor: 49.962

3.  A novel strategy for the assignment of side-chain resonances in completely deuterated large proteins using 13C spectroscopy.

Authors:  Alexander Eletsky; Osvaldo Moreira; Helena Kovacs; Konstantin Pervushin
Journal:  J Biomol NMR       Date:  2003-06       Impact factor: 2.835

4.  Use of protonless NMR spectroscopy to alleviate the loss of information resulting from exchange-broadening.

Authors:  Shang-Te Danny Hsu; Carlos W Bertoncini; Christopher M Dobson
Journal:  J Am Chem Soc       Date:  2009-06-03       Impact factor: 15.419

5.  Speeding up (13)C direct detection biomolecular NMR spectroscopy.

Authors:  Wolfgang Bermel; Ivano Bertini; Isabella C Felli; Roberta Pierattelli
Journal:  J Am Chem Soc       Date:  2009-10-28       Impact factor: 15.419

6.  Carbon-detected NMR experiments to investigate structure and dynamics of biological macromolecules.

Authors:  Z Serber; C Richter; V Dötsch
Journal:  Chembiochem       Date:  2001-04-02       Impact factor: 3.164

7.  NMR experiments for resonance assignments of 13C, 15N doubly-labeled flexible polypeptides: application to the human prion protein hPrP(23-230).

Authors:  A Liu; R Riek; G Wider; C von Schroetter; R Zahn; K Wüthrich
Journal:  J Biomol NMR       Date:  2000-02       Impact factor: 2.835

8.  High-resolution 3D CANCA NMR experiments for complete mainchain assignments using C(alpha) direct detection.

Authors:  Koh Takeuchi; Dominique P Frueh; Sven G Hyberts; Zhen-Yu J Sun; Gerhard Wagner
Journal:  J Am Chem Soc       Date:  2010-03-10       Impact factor: 15.419

9.  Poisson-gap sampling and forward maximum entropy reconstruction for enhancing the resolution and sensitivity of protein NMR data.

Authors:  Sven G Hyberts; Koh Takeuchi; Gerhard Wagner
Journal:  J Am Chem Soc       Date:  2010-02-24       Impact factor: 15.419

10.  Selective backbone labeling of proteins using 1,2-13C2-pyruvate as carbon source.

Authors:  Chenyun Guo; Chun Geng; Vitali Tugarinov
Journal:  J Biomol NMR       Date:  2009-05-26       Impact factor: 2.835
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  11 in total

1.  4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins.

Authors:  Barbara Krähenbühl; Sebastian Hiller; Gerhard Wider
Journal:  J Biomol NMR       Date:  2011-09-27       Impact factor: 2.835

2.  Paramagnetic relaxation enhancement to improve sensitivity of fast NMR methods: application to intrinsically disordered proteins.

Authors:  François-Xavier Theillet; Andres Binolfi; Stamatis Liokatis; Silvia Verzini; Philipp Selenko
Journal:  J Biomol NMR       Date:  2011-10-19       Impact factor: 2.835

3.  Improving the chemical shift dispersion of multidimensional NMR spectra of intrinsically disordered proteins.

Authors:  Wolfgang Bermel; Marta Bruix; Isabella C Felli; Vasantha Kumar M V; Roberta Pierattelli; Soraya Serrano
Journal:  J Biomol NMR       Date:  2013-01-12       Impact factor: 2.835

4.  A new broadband homonuclear mixing pulse for NMR with low applied power.

Authors:  Paul Coote; Kendra E Leigh; Tsyr-Yan Yu; Navin Khaneja; Gerhard Wagner; Haribabu Arthanari
Journal:  J Chem Phys       Date:  2014-07-14       Impact factor: 3.488

5.  Improved NMR experiments with ¹³C-isotropic mixing for assignment of aromatic and aliphatic side chains in labeled proteins.

Authors:  Helena Kovacs; Alvar Gossert
Journal:  J Biomol NMR       Date:  2014-01-04       Impact factor: 2.835

Review 6.  Emerging solution NMR methods to illuminate the structural and dynamic properties of proteins.

Authors:  Haribabu Arthanari; Koh Takeuchi; Abhinav Dubey; Gerhard Wagner
Journal:  Curr Opin Struct Biol       Date:  2019-07-19       Impact factor: 6.809

7.  HNCA-TOCSY-CANH experiments with alternate (13)C- (12)C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment.

Authors:  Koh Takeuchi; Maayan Gal; Hideo Takahashi; Ichio Shimada; Gerhard Wagner
Journal:  J Biomol NMR       Date:  2010-11-26       Impact factor: 2.835

8.  Selective (15)N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy.

Authors:  Chan Cao; Jia-Liang Chen; Yin Yang; Feng Huang; Gottfried Otting; Xun-Cheng Su
Journal:  J Biomol NMR       Date:  2014-07-08       Impact factor: 2.835

Review 9.  13C Direct Detected NMR for Challenging Systems.

Authors:  Isabella C Felli; Roberta Pierattelli
Journal:  Chem Rev       Date:  2022-01-13       Impact factor: 72.087

Review 10.  Modern Technologies of Solution Nuclear Magnetic Resonance Spectroscopy for Three-dimensional Structure Determination of Proteins Open Avenues for Life Scientists.

Authors:  Toshihiko Sugiki; Naohiro Kobayashi; Toshimichi Fujiwara
Journal:  Comput Struct Biotechnol J       Date:  2017-04-13       Impact factor: 7.271
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