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Literature DB >> 20382479

Characterization of a direct detection device imaging camera for transmission electron microscopy.

Anna-Clare Milazzo¹, Grigore Moldovan, Jason Lanman, Liang Jin, James C Bouwer, Stuart Klienfelder, Steven T Peltier, Mark H Ellisman, Angus I Kirkland, Nguyen-Huu Xuong.

Abstract

The complete characterization of a novel direct detection device (DDD) camera for transmission electron microscopy is reported, for the first time at primary electron energies of 120 and 200 keV. Unlike a standard charge coupled device (CCD) camera, this device does not require a scintillator. The DDD transfers signal up to 65 lines/mm providing the basis for a high-performance platform for a new generation of wide field-of-view high-resolution cameras. An image of a thin section of virus particles is presented to illustrate the substantially improved performance of this sensor over current indirectly coupled CCD cameras. Copyright 2010 Elsevier B.V. All rights reserved.

Entities: Disease Species

Mesh：

Year: 2010 PMID： 20382479 PMCID： PMC2923635 DOI： 10.1016/j.ultramic.2010.03.007

Source DB: PubMed Journal: Ultramicroscopy ISSN： 0304-3991 Impact factor: 2.689

12 in total

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Journal: Microsc Res Tech Date: 2000-05-01 Impact factor: 2.769

3. Experimental characterisation of CCD cameras for HREM at 300 kV

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Journal: Ultramicroscopy Date: 2000-09 Impact factor: 2.689

4. Direct electron imaging in electron microscopy with monolithic active pixel sensors.

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Journal: Ultramicroscopy Date: 2007-01-28 Impact factor: 2.689

5. A charge coupled device camera with electron decelerator for intermediate voltage electron microscopy.

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Journal: Rev Sci Instrum Date: 2008-04 Impact factor: 1.523

6. A modified method for lead staining of thin sections.

Authors: T Sato
Journal: J Electron Microsc (Tokyo) Date: 1968

7. Visualizing flock house virus infection in Drosophila cells with correlated fluorescence and electron microscopy.

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8. Experimental observation of the improvement in MTF from backthinning a CMOS direct electron detector.

Authors: G McMullan; A R Faruqi; R Henderson; N Guerrini; R Turchetta; A Jacobs; G van Hoften
Journal: Ultramicroscopy Date: 2009-05-18 Impact factor: 2.689

9. The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.

Authors: Gina E Sosinsky; John Crum; Ying Z Jones; Jason Lanman; Benjamin Smarr; Masako Terada; Maryann E Martone; Thomas J Deerinck; John E Johnson; Mark H Ellisman
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10. Detective quantum efficiency of electron area detectors in electron microscopy.

Authors: G McMullan; S Chen; R Henderson; A R Faruqi
Journal: Ultramicroscopy Date: 2009-05-07 Impact factor: 2.689

19 in total

1. Initial evaluation of a direct detection device detector for single particle cryo-electron microscopy.

Authors: Anna-Clare Milazzo; Anchi Cheng; Arne Moeller; Dmitry Lyumkis; Erica Jacovetty; James Polukas; Mark H Ellisman; Nguyen-Huu Xuong; Bridget Carragher; Clinton S Potter
Journal: J Struct Biol Date: 2011-09-10 Impact factor: 2.867

2. Resolution measures in molecular electron microscopy.

Authors: Pawel A Penczek
Journal: Methods Enzymol Date: 2010 Impact factor: 1.600

3. Direct electron detection yields cryo-EM reconstructions at resolutions beyond 3/4 Nyquist frequency.

Authors: Benjamin E Bammes; Ryan H Rochat; Joanita Jakana; Dong-Hua Chen; Wah Chiu
Journal: J Struct Biol Date: 2012-01-21 Impact factor: 2.867

4. CTER-rapid estimation of CTF parameters with error assessment.

Authors: Pawel A Penczek; Jia Fang; Xueming Li; Yifan Cheng; Justus Loerke; Christian M T Spahn
Journal: Ultramicroscopy Date: 2014-02-07 Impact factor: 2.689

Review 5. Confessions of an icosahedral virus crystallographer.

Authors: John E Johnson
Journal: Microscopy (Oxf) Date: 2013-01-04 Impact factor: 1.571

6. Practical performance evaluation of a 10k × 10k CCD for electron cryo-microscopy.

Authors: Benjamin E Bammes; Ryan H Rochat; Joanita Jakana; Wah Chiu
Journal: J Struct Biol Date: 2011-05-17 Impact factor: 2.867

Review 7. Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography.

Authors: Juan Chang; Xiangan Liu; Ryan H Rochat; Matthew L Baker; Wah Chiu
Journal: Adv Exp Med Biol Date: 2012 Impact factor: 2.622

Review 8. Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions.

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9. Cryo-electron tomography for structural characterization of macromolecular complexes.

Authors: Julia Cope; John Heumann; Andreas Hoenger
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10. Improving signal to noise in labeled biological specimens using energy-filtered TEM of sections with a drift correction strategy and a direct detection device.

Authors: Ranjan Ramachandra; James C Bouwer; Mason R Mackey; Eric Bushong; Steven T Peltier; Nguyen-Huu Xuong; Mark H Ellisman
Journal: Microsc Microanal Date: 2014-03-19 Impact factor: 4.127