Literature DB >> 7765788

Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, Forskolin.

R Sakata1, Y Shoyama, H Murakami.   

Abstract

The ratio of hapten to bovine serum albumin in an antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against forskolin was produced by fusing splenocytes immunized with a forskolin-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cells. The cross-reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.57%. A very small cross-reaction appeared with 1-deoxy, 9-deoxy and 1,9-dideoxy forskolin derivatives. The full measuring range of the assay extends from 6 ng to 200 ng of forskolin. The competitive ELISA assay used for this analysis was found to be more sensitive than TLC (10 micrograms), GLC (30 ng) and HPLC (1 microgram) methods.

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Year:  1994        PMID： 7765788     DOI： 10.1007/BF00754612

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  15 in total

1.  Establishment and characterization of monoclonal antibodies against Chuzan virus K-47.

Authors:  N Eto; K Yamada; A Koga; S Shirahata; H Murakami
Journal:  Cytotechnology       Date:  1991-05       Impact factor: 2.058

2.  Variation in forskolin content in the roots of Coleus forskohlii.

Authors:  R A Vishwakarma; B R Tyagi; B Ahmed; A Husain
Journal:  Planta Med       Date:  1988-10       Impact factor: 3.352

3.  Production of Forskolin by Axenic Coleus forskohlii Roots Cultivated in Shake Flasks and 20-l Glass Jar Bioreactors*.

Authors:  R Krombholz; R Mersinger; W Kreis; E Reinhard
Journal:  Planta Med       Date:  1992-08       Impact factor: 3.352

4.  Identification of viruses infected in Rehmannia glutinosa Libosch. var purpurea Makino and effect of virus infection on root yield and iridoid glycoside contents.

Authors:  M Matsumoto; Y Shoyama; I Nishioka; H Iwai; S Wakimoto
Journal:  Plant Cell Rep       Date:  1989-03       Impact factor: 4.570

5.  Formation of forskolin by suspension cultures of Coleus forskohlii.

Authors:  R Mersinger; H Dornauer; E Reinhard
Journal:  Planta Med       Date:  1988-06       Impact factor: 3.352

6.  GLC method for assay of forskolin, a novel positive inotropic and blood pressure-lowering agent.

Authors:  P K Inamdar; H Dornauer; N J de Souza
Journal:  J Pharm Sci       Date:  1980-12       Impact factor: 3.534

7.  The positive inotropic-acting forskolin, a potent adenylate cyclase activator.

Authors:  H Metzger; E Lindner
Journal:  Arzneimittelforschung       Date:  1981

8.  Catalytic unit of adenlyate cyclase: purification and identification by affinity crosslinking.

Authors:  E Pfeuffer; R M Dreher; H Metzger; T Pfeuffer
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

9.  Quantitative Determination of Forskolin by TLC and HPLC.

Authors:  P K Inamdar; P V Kanitkar; J Reden; N J de Souza
Journal:  Planta Med       Date:  1984-02       Impact factor: 3.352
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  14 in total

1.  Production of monoclonal antibodies and ELISA for thebaine and codeine.

Authors:  Y Shoyama; T Fukada; H Murakami
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

2.  Production of monoclonal antibodies and development of an ELISA for solamargine.

Authors:  M Ishiyama; Y Shoyama; H Murakami; H Shinohara
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

3.  Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

Authors:  H Tanaka; N Fukuda; Y Shoyama
Journal:  Cytotechnology       Date:  1999-03       Impact factor: 2.058

4.  Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies.

Authors:  Boonchoo Sritularak; Osamu Morinaga; Chun-Su Yuan; Yukihiro Shoyama; Hiroyuki Tanaka
Journal:  J Nat Med       Date:  2009-04-18       Impact factor: 2.343

5.  Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody.

Authors:  W Putalun; H Tanaka; Y Shoyama
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

6.  Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.

Authors:  N Fukuda; H Tanaka; Y Shoyama
Journal:  Cytotechnology       Date:  2000-11       Impact factor: 2.058

7.  Production of monoclonal antibodies against antitumor cyclohexapeptide RA-VII from Rubia cordifolia and their characterization.

Authors:  Tomoyo Hasuda; Yukio Hitotsuyanagi; Koichi Takeya
Journal:  J Nat Med       Date:  2011-02-08       Impact factor: 2.343

8.  Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.

Authors:  Hiroyuki Tanaka; Shayu Yan; Norio Miura; Yukihiro Shoyama
Journal:  Cytotechnology       Date:  2003-07       Impact factor: 2.058

9.  Monoclonal antibodies against naturally occurring bioactive compounds.

Authors:  Y Shoyama; H Tanaka; N Fukuda
Journal:  Cytotechnology       Date:  1999-09       Impact factor: 2.058

10.  Preparation of monoclonal antibody against crocin and its characterization.

Authors:  L Xuan; H Tanaka; Y Xu; Y Shoyama
Journal:  Cytotechnology       Date:  1999-01       Impact factor: 2.058
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