Literature DB >> 22359060

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

H Tanaka1, N Fukuda, Y Shoyama.   

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

Entities:  

Year:  1999        PMID： 22359060      PMCID： PMC3449911          DOI： 10.1023/A:1008068718392

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  11 in total

1.  ANTIBODIES SPECIFIC FOR RIBONUCLEOSIDES AND RIBONUCLEOTIDES AND THEIR REACTION WITH DNA.

Authors:  B F ERLANGER; S M BEISER
Journal:  Proc Natl Acad Sci U S A       Date:  1964-07       Impact factor: 11.205

2.  Production of monoclonal antibodies and ELISA for thebaine and codeine.

Authors:  Y Shoyama; T Fukada; H Murakami
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

3.  Production of monoclonal antibodies and development of an ELISA for solamargine.

Authors:  M Ishiyama; Y Shoyama; H Murakami; H Shinohara
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

4.  High-performance liquid chromatographic analysis of acidic saponins of ginseng and related plants.

Authors:  H Yamaguchi; R Kasai; H Matsuura; O Tanaka; T Fuwa
Journal:  Chem Pharm Bull (Tokyo)       Date:  1988-09       Impact factor: 1.645

5.  Characterization of two novel polysaccharides having immunological activities from the root of Panax ginseng.

Authors:  M Tomoda; K Hirabayashi; N Shimizu; R Gonda; N Ohara; K Takada
Journal:  Biol Pharm Bull       Date:  1993-11       Impact factor: 2.233

6.  [Simultaneous analysis of ginsenosides of various ginseng radix by HPLC].

Authors:  K Samukawa; H Yamashita; H Matsuda; M Kubo
Journal:  Yakugaku Zasshi       Date:  1995-03       Impact factor: 0.302

7.  Cannabis, 21. Biotransformation of cannabinol to its glycosides by in vitro plant tissue.

Authors:  H Tanaka; S Morimoto; Y Shoyama
Journal:  J Nat Prod       Date:  1993-12       Impact factor: 4.050

8.  [HPLC separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from Ginseng drug preparations (author's transl)].

Authors:  O Sticher; F Soldati
Journal:  Planta Med       Date:  1979-05       Impact factor: 3.352

9.  HPLC separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng drug preparations. 2nd communication.

Authors:  F Soldati; O Sticher
Journal:  Planta Med       Date:  1980-08       Impact factor: 3.352

10.  [Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2): Comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root].

Authors:  I Kitagawa; T Taniyama; H Shibuya; T Noda; M Yoshikawa
Journal:  Yakugaku Zasshi       Date:  1987-07       Impact factor: 0.302
View more
  10 in total

1.  Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies.

Authors:  Boonchoo Sritularak; Osamu Morinaga; Chun-Su Yuan; Yukihiro Shoyama; Hiroyuki Tanaka
Journal:  J Nat Med       Date:  2009-04-18       Impact factor: 2.343

2.  Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.

Authors:  N Fukuda; H Tanaka; Y Shoyama
Journal:  Cytotechnology       Date:  2000-11       Impact factor: 2.058

3.  Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs.

Authors:  Benyakan Pongkitwitoon; Seiichi Sakamoto; Osamu Morinaga; Thaweesak Juengwatanatrakul; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2010-08-14       Impact factor: 2.343

4.  Pharmacology of ginsenosides: a literature review.

Authors:  Kar Wah Leung; Alice Sze-Tsai Wong
Journal:  Chin Med       Date:  2010-06-11       Impact factor: 5.455

5.  Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.

Authors:  Hiroyuki Tanaka; Shayu Yan; Norio Miura; Yukihiro Shoyama
Journal:  Cytotechnology       Date:  2003-07       Impact factor: 2.058

6.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

7.  K-targeted metabolomic analysis extends chemical subtraction to DESIGNER extracts: selective depletion of extracts of hops (Humulus lupulus).

Authors:  René F Ramos Alvarenga; J Brent Friesen; Dejan Nikolić; Charlotte Simmler; José G Napolitano; Richard van Breemen; David C Lankin; James B McAlpine; Guido F Pauli; Shao-Nong Chen
Journal:  J Nat Prod       Date:  2014-12-01       Impact factor: 4.050

Review 8.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06

Review 9.  Lateral flow immunoassay for small-molecules detection in phytoproducts: a review.

Authors:  Poomraphie Nuntawong; Waraporn Putalun; Hiroyuki Tanaka; Satoshi Morimoto; Seiichi Sakamoto
Journal:  J Nat Med       Date:  2022-02-16       Impact factor: 3.192

Review 10.  Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.

Authors:  Seiichi Sakamoto; Waraporn Putalun; Sornkanok Vimolmangkang; Waranyoo Phoolcharoen; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2017-11-21       Impact factor: 2.343
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.