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Literature DB >> 19003395

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.

Abstract

The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg(1) was produced by fusing sprenocytes immunized with aginsenoside Rg(1)-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 mug ml(-1) to 10 mug ml(-1) ofginsenoside Rg(1).

Entities: Chemical Disease Species

Year: 2000 PMID： 19003395 PMCID： PMC3449635 DOI： 10.1023/A:1008162703957

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

20 in total

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Journal: Cytotechnology Date: 1995-01 Impact factor: 2.058

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Journal: Cytotechnology Date: 1995-01 Impact factor: 2.058

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Authors: H Tanaka; N Fukuda; Y Shoyama
Journal: Cytotechnology Date: 1999-03 Impact factor: 2.058

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Journal: Proc Natl Acad Sci U S A Date: 1982-02 Impact factor: 11.205

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Journal: Yakugaku Zasshi Date: 1987-07 Impact factor: 0.302

10. Preparation of monoclonal antibody against crocin and its characterization.

Authors: L Xuan; H Tanaka; Y Xu; Y Shoyama
Journal: Cytotechnology Date: 1999-01 Impact factor: 2.058

12 in total

1. Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies.

Authors: Boonchoo Sritularak; Osamu Morinaga; Chun-Su Yuan; Yukihiro Shoyama; Hiroyuki Tanaka
Journal: J Nat Med Date: 2009-04-18 Impact factor: 2.343

2. Protective effect of ginsenosides Rg1 and Re on lipopolysaccharide-induced sepsis by competitive binding to Toll-like receptor 4.

Authors: Fei Su; Yin Xue; Yuemin Wang; Lili Zhang; Wangxue Chen; Songhua Hu
Journal: Antimicrob Agents Chemother Date: 2015-07-06 Impact factor: 5.191

Review 3. Isolation and analysis of ginseng: advances and challenges.

Authors: Lian-Wen Qi; Chong-Zhi Wang; Chun-Su Yuan
Journal: Nat Prod Rep Date: 2011-01-24 Impact factor: 13.423

4. Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs.

Authors: Benyakan Pongkitwitoon; Seiichi Sakamoto; Osamu Morinaga; Thaweesak Juengwatanatrakul; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal: J Nat Med Date: 2010-08-14 Impact factor: 2.343

5. Establishment of indirect competitive enzyme-linked immunosorbent assay for the detection of platycodin D in Radix Platycodonis.

Authors: Shuai Luo; Yanfei He; Hongxiang Sun
Journal: J Food Drug Anal Date: 2020-09-15 Impact factor: 6.157

6. Pharmacology of ginsenosides: a literature review.

Authors: Kar Wah Leung; Alice Sze-Tsai Wong
Journal: Chin Med Date: 2010-06-11 Impact factor: 5.455

7. Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.

Authors: Hiroyuki Tanaka; Shayu Yan; Norio Miura; Yukihiro Shoyama
Journal: Cytotechnology Date: 2003-07 Impact factor: 2.058

8. Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors: Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal: Int J Anal Chem Date: 2012-02-14 Impact factor: 1.885

Review 9. Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review.

Authors: Xin Yan; Yan Zhao; Yue Zhang; Huihua Qu
Journal: Molecules Date: 2017-02-26 Impact factor: 4.411

Review 10. Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.

Authors: Seiichi Sakamoto; Waraporn Putalun; Sornkanok Vimolmangkang; Waranyoo Phoolcharoen; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal: J Nat Med Date: 2017-11-21 Impact factor: 2.343