Literature DB >> 19003135

Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody.

W Putalun, H Tanaka, Y Shoyama.   

Abstract

Immunoaffinity column using anti-solamargine monoclonal antibody for separation of solasodine glycosides was established. This method was specific for solasodine glycosides which was detected by thin layer chromatography and the western blotting. Total solasodine glycosides have been separated directly from the crude extract of Solanum khasianum fruit by the newly established immunoaffinity column.

Entities:  

Year:  1999        PMID： 19003135      PMCID： PMC3449769          DOI： 10.1023/A:1008032524419

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  5 in total

1.  Production of monoclonal antibodies and development of an ELISA for solamargine.

Authors:  M Ishiyama; Y Shoyama; H Murakami; H Shinohara
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

2.  A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine.

Authors:  H Tanaka; W Putalun; C Tsuzaki; Y Shoyama
Journal:  FEBS Lett       Date:  1997-03-10       Impact factor: 4.124

3.  Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, Forskolin.

Authors:  R Sakata; Y Shoyama; H Murakami
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

4.  Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry.

Authors:  Y Shoyama; T Fukada; T Tanaka; A Kusai; K Nojima
Journal:  Biol Pharm Bull       Date:  1993-10       Impact factor: 2.233

5.  A simple and quantitative purification of glycosphingolipids and phospholipids by thin-layer chromatography blotting.

Authors:  T Taki; T Kasama; S Handa; D Ishikawa
Journal:  Anal Biochem       Date:  1994-12       Impact factor: 3.365
  5 in total
  4 in total

1.  Anti-solasodine glycoside single-chain Fv antibody stimulates biosynthesis of solasodine glycoside in plants.

Authors:  W Putalun; F Taura; W Qing; H Matsushita; H Tanaka; Y Shoyama
Journal:  Plant Cell Rep       Date:  2003-09-20       Impact factor: 4.570

2.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

Review 3.  Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review.

Authors:  Xin Yan; Yan Zhao; Yue Zhang; Huihua Qu
Journal:  Molecules       Date:  2017-02-26       Impact factor: 4.411

Review 4.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06
  4 in total

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