Literature DB >> 19003338

Preparation of monoclonal antibody against crocin and its characterization.

L Xuan1, H Tanaka, Y Xu, Y Shoyama.   

Abstract

Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. They were identified as IgG2a and IgG2b possessing lambda light chain, respectively. Their wide reactivities against crocetin glycosides were discussed.

Entities:  

Year:  1999        PMID： 19003338      PMCID： PMC3449468          DOI： 10.1023/A:1007993615489

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  15 in total

1.  [Carotenoid-glycosides. Investigation of carotenoid-composition of saffron].

Authors:  H Pfander; F Wittwer
Journal:  Helv Chim Acta       Date:  1975-09-24       Impact factor: 2.164

2.  Crocin, safranal and picrocrocin from saffron (Crocus sativus L.) inhibit the growth of human cancer cells in vitro.

Authors:  J Escribano; G L Alonso; M Coca-Prados; J A Fernandez
Journal:  Cancer Lett       Date:  1996-02-27       Impact factor: 8.679

3.  Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component.

Authors:  H Murakami; H Masui; G H Sato; N Sueoka; T P Chow; T Kano-Sueoka
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

4.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

5.  Inhibitory effect of crocetin on intracellular nucleic acid and protein synthesis in malignant cells.

Authors:  F I Abdullaev
Journal:  Toxicol Lett       Date:  1994-02-01       Impact factor: 4.372

6.  Crocin (crocetin di-gentiobiose ester) prevents the inhibitory effect of ethanol on long-term potentiation in the dentate gyrus in vivo.

Authors:  M Sugiura; Y Shoyama; H Saito; K Abe
Journal:  J Pharmacol Exp Ther       Date:  1994-11       Impact factor: 4.030

7.  Effects of Crocus sativus L. on the ethanol-induced impairment of passive avoidance performances in mice.

Authors:  Y Zhang; Y Shoyama; M Sugiura; H Saito
Journal:  Biol Pharm Bull       Date:  1994-02       Impact factor: 2.233

8.  Post-harvest degradation of carotenoid glucose esters in saffron.

Authors:  S Morimoto; Y Umezaki; Y Shoyama; H Saito; K Nishi; N Irino
Journal:  Planta Med       Date:  1994-10       Impact factor: 3.352

9.  Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, Forskolin.

Authors:  R Sakata; Y Shoyama; H Murakami
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

10.  Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry.

Authors:  Y Shoyama; T Fukada; T Tanaka; A Kusai; K Nojima
Journal:  Biol Pharm Bull       Date:  1993-10       Impact factor: 2.233
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  8 in total

1.  Preparation and purification of monoclonal antibodies against chloramphenicol.

Authors:  Yu Yi; Zhuhuan Wang; Min Li; Keyin Zhu; Guoqing Ying
Journal:  Cytotechnology       Date:  2011-12-11       Impact factor: 2.058

2.  Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin.

Authors:  N Fukuda; H Tanaka; Y Shoyama
Journal:  Cytotechnology       Date:  2000-11       Impact factor: 2.058

3.  New minor glycoside components from saffron.

Authors:  Nguyen Huu Tung; Yukihiro Shoyama
Journal:  J Nat Med       Date:  2012-11-21       Impact factor: 2.343

4.  Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies.

Authors:  Hiroyuki Tanaka; Shayu Yan; Norio Miura; Yukihiro Shoyama
Journal:  Cytotechnology       Date:  2003-07       Impact factor: 2.058

5.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

Review 6.  Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review.

Authors:  Xin Yan; Yan Zhao; Yue Zhang; Huihua Qu
Journal:  Molecules       Date:  2017-02-26       Impact factor: 4.411

Review 7.  Preventing Dementia Using Saffron, The Kampo Medicine, Kamiuntanto, and Their Combination, Kamiuntantokabankoka.

Authors:  Kenny Kuchta; Kosuke Aritake; Yoshihiro Urade; Nguyen Huu Tung; Chun-Su Yuan; Yui Sasaki; Koichi Shimizu; Yukihiro Shoyama
Journal:  Front Pharmacol       Date:  2022-03-04       Impact factor: 5.810

Review 8.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06
  8 in total

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