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Literature DB >> 1369335

Establishment and characterization of monoclonal antibodies against Chuzan virus K-47.

N Eto¹, K Yamada, A Koga, S Shirahata, H Murakami.

Abstract

We established sixteen mouse monoclonal antibodies reactive to Chuzan virus K-47 strain using P3-X63-Ag8-U1 cells as fusion partner cells. Among them, CG53/2/4 recognized a 100K structural protein of the virus. The 100K antigen lost it's antigenicity for CG53/2/4 after mild periodate oxidation treatment, suggesting that the 100K viral antigen is a glycoprotein. In addition, CG53/2/4 neutralized the viral infectivity. This indicates that the 100K glycoprotein is essential for the infection of the virus. The other monoclonal antibodies reacted with a 41K antigen of the virus. Especially CG1/1 showed the highest reactivity to the virus. Forward step sandwich assay using CG1/1 and biotinylated CG53/2/4 could detect the virus at 10TCID50/ml. Therefore, these monoclonal antibodies can eventually predict the virus infection to the animals before their sideration.

Entities: Chemical Species

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Year: 1991 PMID： 1369335 DOI： 10.1007/bf00353698

Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058

20 in total

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Authors: Y Goto; Y Miura; Y Kono
Journal: Nihon Juigaku Zasshi Date: 1988-04

6. Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component.

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Journal: J Immunol Methods Date: 1980 Impact factor: 2.303

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9. Ibaraki virus, an agent of epizootic disease of cattle resembling bluetongue. II. Isolation of the virus in bovine cell culture.

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Journal: Jpn J Microbiol Date: 1969-06

10. Serologic evidence for the etiologic role of Chuzan virus in an epizootic of congenital abnormalities with hydranencephaly-cerebellar hypoplasia syndrome of calves in Japan.

Authors: Y Goto; Y Miura; Y Kono
Journal: Am J Vet Res Date: 1988-12 Impact factor: 1.156

4 in total