Literature DB >> 6171820

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

S V Suggs, R B Wallace, T Hirose, E H Kawashima, K Itakura.   

Abstract

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One beta 2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned beta 2-microglobulin sequence contains 217 base pairs of the 3' untranslated region of the mRNA and 328 base pairs (97%) of the coding region.

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Year:  1981        PMID： 6171820      PMCID： PMC349099          DOI： 10.1073/pnas.78.11.6613

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  The base-pairing specificity of cellulose-pdT9.

Authors:  S Gillam; K Waterman; M Smith
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2.  The complete amino acid sequence of beta 2-microglobulin.

Authors:  B A Cunningham; J L Wang; I Berggård; P A Peterson
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3.  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).

Authors:  M A Epstein; B G Achong; Y M Barr; B Zajac; G Henle; W Henle
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4.  Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids.

Authors:  I Berggård; A G Bearn
Journal:  J Biol Chem       Date:  1968-08-10       Impact factor: 5.157

5.  Messenger RNA in HeLa cells: kinetics of formation and decay.

Authors:  R H Singer; S Penman
Journal:  J Mol Biol       Date:  1973-08-05       Impact factor: 5.469

6.  Further studies on the properties of oligonucleotide cellulose columns.

Authors:  C R Astell; M T Doel; P A Jahnke; M Smith
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

7.  Synthesis and properties of oligonucleotide--cellulose columns.

Authors:  C R Astell; M Smith
Journal:  Biochemistry       Date:  1972-10-24       Impact factor: 3.162

8.  Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.

Authors:  J R Parnes; B Velan; A Felsenfeld; L Ramanathan; U Ferrini; E Appella; J G Seidman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

9.  Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules.

Authors:  K Nakamuro; N Tanigaki; D Pressman
Journal:  Proc Natl Acad Sci U S A       Date:  1973-10       Impact factor: 11.205

10.  Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin.

Authors:  P A Peterson; L Rask; J B Lindblom
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205
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  148 in total

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Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857

4.  A reliable method for the use of oligonucleotides as probes in blot-hybridization experiments.

Authors:  J Paquette; C Sapienza
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

5.  Identification of a shark sequence resembling the major histocompatibility complex class I alpha 3 domain.

Authors:  K Hashimoto; T Nakanishi; Y Kurosawa
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

6.  Frequency and organization of papA homologous DNA sequences among uropathogenic digalactoside-binding Escherichia coli strains.

Authors:  K Denich; A Craiu; H Rugo; G Muralidhar; P O'Hanley
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

7.  Characterization and gestational regulation of corticotropin-releasing hormone messenger RNA in human placenta.

Authors:  D M Frim; R L Emanuel; B G Robinson; C M Smas; G K Adler; J A Majzoub
Journal:  J Clin Invest       Date:  1988-07       Impact factor: 14.808

8.  Human genomic library screened with 17-base oligonucleotide probes yields a novel interferon gene.

Authors:  R M Torczynski; M Fuke; A P Bollon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  Domain structures and molecular evolution of class I and class II major histocompatibility gene complex (MHC) products deduced from amino acid and nucleotide sequence homologies.

Authors:  K Ohnishi
Journal:  Orig Life       Date:  1984

10.  Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.

Authors:  D E Woods; A F Markham; A T Ricker; G Goldberger; H R Colten
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205
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