Literature DB >> 4059054

Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside.

H Inoue, A Imura, E Ohtsuka.   

Abstract

Partially self-complementary dodecadeoxyribonucleotides containing a fluorescent nucleoside, 3-beta-D-2'-deoxyribofuranosyl-2, 7-dioxopyrido[2, 3-d]pyrimidine (pyridopyrimidine deoxynucleoside, dF) were synthesized by the phosphotriester solidphase method. A dodecanucleotide d(GGGAAFGTTCCC) pairing the analog and guanine at the centre of the chain showed a higher melting temperature than the corresponding G-C paired duplex. A similar comparison between A-T and A-F suggested that weaker hydrogen bonds exist when adenine and pyridopyrimidine residues are paired.

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Year:  1985        PMID： 4059054      PMCID： PMC322027          DOI： 10.1093/nar/13.19.7119

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Joining of 3'-modified oligonucleotides by T4 RNA ligase. Synthesis of a heptadecanucleotide corresponding to the bases 61--77 from Escherichia coli tRNAfMet.

Authors:  E Ohtsuka; S Nishikawa; A F Markham; S Tanaka; T Miyake; T Wakabayashi; M Ikehara; M Sugiura
Journal:  Biochemistry       Date:  1978-11-14       Impact factor: 3.162

2.  An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.

Authors:  E Ohtsuka; S Matsuki; M Ikehara; Y Takahashi; K Matsubara
Journal:  J Biol Chem       Date:  1985-03-10       Impact factor: 5.157

3.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

4.  Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA.

Authors:  F Sanger; J E Donelson; A R Coulson; H Kössel; D Fischer
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

5.  Synthesis of a gene for human growth hormone and its expression in Escherichia coli.

Authors:  M Ikehara; E Ohtsuka; T Tokunaga; Y Taniyama; S Iwai; K Kitano; S Miyamoto; T Ohgi; Y Sakuragawa; K Fujiyama
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

6.  Synthesis and biophysical studies of short oligodeoxynucleotides with novel modifications: a possible approach to the problem of mixed base oligodeoxynucleotide synthesis.

Authors:  T A Millican; G A Mock; M A Chauncey; T P Patel; M A Eaton; J Gunning; S D Cutbush; S Neidle; J Mann
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971

7.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
  7 in total
  11 in total

1.  NMR structure of a DNA duplex containing nucleoside analog 1-(2'-deoxy-beta-D-ribofuranosyl)-3-nitropyrrole and the structure of the unmodified control.

Authors:  D A Klewer; A Hoskins; P Zhang; V J Davisson; D E Bergstrom; A C LiWang
Journal:  Nucleic Acids Res       Date:  2000-11-15       Impact factor: 16.971

2.  Preorganization of DNA: Design Principles for Improving Nucleic Acid Recognition by Synthetic Oligonucleotides.

Authors:  Eric T. Kool
Journal:  Chem Rev       Date:  1997-08-05       Impact factor: 60.622

3.  Factors Contributing to Aromatic Stacking in Water: Evaluation in the Context of DNA.

Authors:  Kevin M Guckian; Barbara A Schweitzer; Rex X-F Ren; Charles J Sheils; Deborah C Tahmassebi; Eric T Kool
Journal:  J Am Chem Soc       Date:  2000-02-10       Impact factor: 15.419

4.  Synthesis and properties of oligonucleotides containing 2'-deoxynebularine and 2'-deoxyxanthosine.

Authors:  R Eritja; D M Horowitz; P A Walker; J P Ziehler-Martin; M S Boosalis; M F Goodman; K Itakura; B E Kaplan
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

5.  Pyrrolo-C as a fluorescent probe for monitoring RNA secondary structure formation.

Authors:  Rebecca A Tinsley; Nils G Walter
Journal:  RNA       Date:  2006-01-23       Impact factor: 4.942

6.  Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.

Authors:  Brittney J Rodgers; Nada A Elsharif; Nisha Vashisht; Macy M Mingus; Mark A Mulvahill; Gudrun Stengel; Robert D Kuchta; Byron W Purse
Journal:  Chemistry       Date:  2013-12-05       Impact factor: 5.236

7.  Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides.

Authors:  H Inoue; Y Hayase; A Imura; S Iwai; K Miura; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

8.  Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.

Authors:  E Jablonski; E W Moomaw; R H Tullis; J L Ruth
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

9.  Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation.

Authors:  A B Staubli; P B Dervan
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

10.  5-Nitroindole as an universal base analogue.

Authors:  D Loakes; D M Brown
Journal:  Nucleic Acids Res       Date:  1994-10-11       Impact factor: 16.971
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