Literature DB >> 450699

Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.

H M Hsiung, R Brousseau, J Michniewicz, S A Narang.   

Abstract

Preparative reversed-phase thin layer chromatography on silanized silica-gel (RP-2 and RP-18) has been developed to purify triester deoxyribooligonucleotides prepared by the modified triester method. The effectiveness of this technique has been demonstrated in the rapid synthesis of eight pure deoxyribooligonucleotides constituting the sequence of human insulin A DNA. The sequence of each of the deoxyribooligonucleotides was confirmed by the two-dimensional mobility-shift method of finger-printing.

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Year:  1979        PMID： 450699      PMCID： PMC327778          DOI： 10.1093/nar/6.4.1371

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  A rapid and convenient synthesis of poly-thymidylic acid by the modified triester approach.

Authors:  A K Sood; S A Narang
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

2.  Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

Authors:  C P Bahl; R Wu; J Stawinsky; S A Narang
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

3.  The phosphotriester approach to oligonucleotide synthesis: Preparation of oligo- and poly-thymidylic acids.

Authors:  R Arentzen; C B Reese
Journal:  J Chem Soc Perkin 1       Date:  1977

4.  Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction.

Authors:  H G Khorana; K L Agarwal; P Besmer; H Büchi; M H Caruthers; P J Cashion; M Fridkin; E Jay; K Kleppe; R Kleppe; A Kumar; P C Loewen; R C Miller; K Minamoto; A Panet; U L RajBhandary; B Ramamoorthy; T Sekiya; T Takeya; J H van de Sande
Journal:  J Biol Chem       Date:  1976-02-10       Impact factor: 5.157

5.  Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide.

Authors:  P C Zamecnik; M L Stephenson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

6.  Nucleoside-3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. III. An improved preparation of nucleoside 3'-phosphotriesters, their 1H NMR characterization and new conditions for removal of 2-cyanoethyl group.

Authors:  R W Adamiak; M Z Barciszewska; E Biala; K Grzéskowiak; R Kierzek; A Kraszewski; W T Markiewicz; M Wiewiórowski
Journal:  Nucleic Acids Res       Date:  1976-12       Impact factor: 16.971

7.  Rat insulin genes: construction of plasmids containing the coding sequences.

Authors:  A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal:  Science       Date:  1977-06-17       Impact factor: 47.728

8.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

9.  Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.

Authors:  K Itakura; T Hirose; R Crea; A D Riggs; H L Heyneker; F Bolivar; H W Boyer
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728

10.  [Synthesis of oligodesoxynucleotides with phosphoric acid triester].

Authors:  F Eckstein; I Rizk
Journal:  Chem Ber       Date:  1969
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  11 in total

1.  Granulocyte-macrophage colony-stimulating factor induces cytokine secretion by human polymorphonuclear leukocytes.

Authors:  A Lindemann; D Riedel; W Oster; H W Ziegler-Heitbrock; R Mertelsmann; F Herrmann
Journal:  J Clin Invest       Date:  1989-04       Impact factor: 14.808

2.  The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.

Authors:  M Houghton; A G Stewart; S M Doel; J S Emtage; M A Eaton; J C Smith; T P Patel; H M Lewis; A G Porter; J R Birch; T Cartwright; N H Carey
Journal:  Nucleic Acids Res       Date:  1980-05-10       Impact factor: 16.971

3.  Deoxydodecanucleotide heteroduplex d(TTTTATAATAAA). d(TTTATTATAAAA) containing the promoter Pribnow sequence TATAAT. I. Double-helix stability by UV spectrophotometry and calorimetry.

Authors:  K Bolewska; A Zielenkiewicz; K L Wierzchowski
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

4.  Chemical synthesis and cloning of a gene for human beta-urogastrone.

Authors:  J Smith; E Cook; I Fotheringham; S Pheby; R Derbyshire; M A Eaton; M Doel; D M Lilley; J F Pardon; T Patel; H Lewis; L D Bell
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

5.  Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

Authors:  M J Gait; M Singh; R C Sheppard; M D Edge; A R Greene; G R Heathcliffe; T C Atkinson; C R Newton; A F Markham
Journal:  Nucleic Acids Res       Date:  1980-03-11       Impact factor: 16.971

6.  Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.

Authors:  W L Sung; H M Hsiung; R Brousseau; J Michniewicz; R Wu; S A Narang
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

7.  Sequence determination of protected oligodeoxyribonucleotides containing phosphotriester linkages by californium-252 plasma desorption mass spectrometry.

Authors:  C J McNeal; S A Narang; R D Macfarlane; H M Hsiung; R Brousseau
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

8.  Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.

Authors:  H M Hsiung; W L Sung; R Brousseau; R Wu; S A Narang
Journal:  Nucleic Acids Res       Date:  1980-12-11       Impact factor: 16.971

9.  The expression in E. coli of synthetic repeating polymeric genes coding for poly(L-aspartyl-L-phenylalanine).

Authors:  M T Doel; M Eaton; E A Cook; H Lewis; T Patel; N H Carey
Journal:  Nucleic Acids Res       Date:  1980-10-24       Impact factor: 16.971

10.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
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