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Literature DB >> 3029671

Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Abstract

The naturally-occurring modified bases, N6-methyladenine, N4-methylcytosine, and 5-methylcytosine were chemically introduced in place of the adenine or cytosine in the decadeoxyribonucleotides containing recognition sequences of Bgl II, Sau 3AI, Mbo I and Mfl I. The modified oligomers bind to the enzymes but the rates of cleavage by the enzymes are variable.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1987 PMID： 3029671 PMCID： PMC340406 DOI： 10.1093/nar/15.1.219

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. The preparation of 6-N-methyldeoxyadenosine.

Authors: A CODDINGTON
Journal: Biochim Biophys Acta Date: 1962-05-21

2. Two restriction endonucleases from Bacillus globiggi.

Authors: V Pirrotta
Journal: Nucleic Acids Res Date: 1976-07 Impact factor: 16.971

3. The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors: M Silberklang; A M Gillum; U L RajBhandary
Journal: Nucleic Acids Res Date: 1977-12 Impact factor: 16.971

4. Two sequence-specific endonucleases from Moraxella bovis.

Authors: R E Gelinas; P A Myers; R J Roberts
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

5. Single-strand and double-strand cleavage at half-modified and fully modified recognition sites for the restriction nucleases Sau3a and Taqi.

Authors: R E Streeck
Journal: Gene Date: 1980-12 Impact factor: 3.688

Review 6. 5-Methylcytosine in eukaryotic DNA.

Authors: M Ehrlich; R Y Wang
Journal: Science Date: 1981-06-19 Impact factor: 47.728

7. Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.

Authors: C H Duncan; G A Wilson; F E Young
Journal: J Bacteriol Date: 1978-04 Impact factor: 3.490

8. EcoRI cleavage and methylation of DNAs containing modified pyrimidines in the recogintion sequence.

Authors: K L Berkner; W R Folk
Journal: J Biol Chem Date: 1977-05-25 Impact factor: 5.157

9. Simplications in the synthesis of short oligonucleotide blocks.

Authors: C Broka; T Hozumi; R Arentzen; K Itakura
Journal: Nucleic Acids Res Date: 1980-11-25 Impact factor: 16.971

10. Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.

Authors: K Miyoshi; R Arentzen; T Huang; K Itakura
Journal: Nucleic Acids Res Date: 1980-11-25 Impact factor: 16.971

17 in total

1. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M McClelland; M Nelson
Journal: Nucleic Acids Res Date: 1992-05-11 Impact factor: 16.971

2. Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1991-04-25 Impact factor: 16.971

3. Synthesis and restriction enzyme analysis of oligodeoxyribonucleotides containing the anti-cancer drug 2',2'-difluoro-2'-deoxycytidine.

Authors: F C Richardson; K K Richardson; J S Kroin; L W Hertel
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

4. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1989 Impact factor: 16.971

Review 5. The effect of site-specific methylation on restriction-modification enzymes.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1987 Impact factor: 16.971

6. Analysis of substrate specificity of the PaeR7 endonuclease: effect of base methylation on the kinetics of cleavage.

Authors: S S Ghosh; P S Obermiller; T J Kwoh; T R Gingeras
Journal: Nucleic Acids Res Date: 1990-09-11 Impact factor: 16.971

7. Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.

Authors: A Fliess; H Wolfes; F Seela; A Pingoud
Journal: Nucleic Acids Res Date: 1988-12-23 Impact factor: 16.971

8. Identification of a methyl-specific restriction system mediated by a conjugative element from Streptomyces bambergiensis.

Authors: S B Zotchev; H Schrempf; C R Hutchinson
Journal: J Bacteriol Date: 1995-08 Impact factor: 3.490

9. Differential effects of the incorporation of 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil (FIAU) on the binding of the transcription factors, AP-1 and TFIID, to their cognate target DNA sequences.

Authors: K A Staschke; K K Richardson; T E Mabry; A J Baxter; J C Scheuring; D M Huffman; W C Smith; F C Richardson; J M Colacino
Journal: Nucleic Acids Res Date: 1996-11-01 Impact factor: 16.971

10. Rapid screening of a human genomic library in yeast artificial chromosomes for single-copy sequences.

Authors: C N Traver; S Klapholz; R W Hyman; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1989-08 Impact factor: 11.205