Literature DB >> 7079181

Studies on deoxyribonucleic acids and related compounds. V. Synthesis of pentadecanucleotide duplex containing the ideal Pribnow sequence of promoter by the phosphotriester method using a new phosphorylating reagent.

E Ohtsuka, Y Taniyama, R Marumoto, H Sato, H Hirosaki, M Ikehara.   

Abstract

A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as 3'-terminal units for the synthesis of oligonucleotide blocks. By condensation of these oligonucleotide blocks the partially complementary deoxypentadecanucleotides dAGCTTATAATGC-TCG and dAGCTCGAGCATTATA, which contained the ideal Pribnow sequence TATAATG, were synthesized.

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Year:  1982        PMID： 7079181      PMCID： PMC320636          DOI： 10.1093/nar/10.8.2597

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  The use of nuclease P1 in sequence analysis of end group labeled RNA.

Authors:  M Silberklang; A M Gillum; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1977-12       Impact factor: 16.971

2.  CV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxyribonucleotide corresponding to the nucleotide sequence 31 to 50.

Authors:  H Büchi; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

3.  Studies on deoxyribonucleic acids and related compounds. III. Synthesis of oligodeoxyribonucleotides complementary to the anticodon loop of Escherichia coli tRNAfmet by an improved triester method.

Authors:  E Ohtsuka; S Shibahara; M Ikehara
Journal:  Chem Pharm Bull (Tokyo)       Date:  1981-12       Impact factor: 1.645

4.  Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Authors:  J Stawinski; T Hozumi; S A Narang; C P Bahl; R Wu
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

5.  Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA.

Authors:  F Sanger; J E Donelson; A R Coulson; H Kössel; D Fischer
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

6.  Simplications in the synthesis of short oligonucleotide blocks.

Authors:  C Broka; T Hozumi; R Arentzen; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971

7.  Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors:  C B Reese; L Zard
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

8.  Studies on transfer ribonucleic acids and related compounds. XL. Synthesis of an eicosaribonucleotide corresponding to residues 35-54 of tRNAfMet from E. coli.

Authors:  E Ohtsuka; K Fujiyama; M Ikehara
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971
  8 in total
  4 in total

1.  Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.

Authors:  T Aoyama; M Takanami; E Ohtsuka; Y Taniyama; R Marumoto; H Sato; M Ikehara
Journal:  Nucleic Acids Res       Date:  1983-09-10       Impact factor: 16.971

2.  Studies on transfer ribonucleic acids and related compounds. XLV. Block condensation of ribooligonucleotides containing 2'-O-tetrahydrofuranyl-5'-O-dimethoxytritylnucleosides.

Authors:  E Ohtsuka; A Yamane; M Ikehara
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

3.  Synthesis of a gene for human growth hormone and its expression in Escherichia coli.

Authors:  M Ikehara; E Ohtsuka; T Tokunaga; Y Taniyama; S Iwai; K Kitano; S Miyamoto; T Ohgi; Y Sakuragawa; K Fujiyama
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

4.  A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of lambda cro binding heptadecanucleotide on a polymer support.

Authors:  E Ohtsuka; Z Tozuka; S Iwai; M Ikehara
Journal:  Nucleic Acids Res       Date:  1982-10-25       Impact factor: 16.971
  4 in total

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