Literature DB >> 7099961

Syringe method for stepwise chemical synthesis of oligonucleotides.

T Tanaka, R L Letsinger.   

Abstract

A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in isolation procedures are also presented.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 7099961      PMCID： PMC320704          DOI： 10.1093/nar/10.10.3249

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Solid support synthesis of oligothymidylates using phosphorochloridates and 1-alkylimidazoles.

Authors:  R C Pless; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1975-06       Impact factor: 16.971

2.  Phosphorylation of tRNA by T4 polynucleotide kinase.

Authors:  J R Lillehaug; K Kleppe
Journal:  Nucleic Acids Res       Date:  1977-02       Impact factor: 16.971

3.  Synthesis of thymidine oligonucleotides by phosphite triester intermediates.

Authors:  R L Letsinger; W B Lunsford
Journal:  J Am Chem Soc       Date:  1976-06-09       Impact factor: 15.419

4.  Automated synthesis of gene fragments.

Authors:  G Alvarado-Urbina; G M Sathe; W C Liu; M F Gillen; P D Duck; R Bender; K K Ogilvie
Journal:  Science       Date:  1981-10-16       Impact factor: 47.728

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Development in the phosphite-triester method of synthesis of oligonucleotides.

Authors:  J L Finnan; A Varshney; R L Letsinger
Journal:  Nucleic Acids Symp Ser       Date:  1980

7.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971
  7 in total
  27 in total

1.  Synthesis and radioiodination of a stannyl oligodeoxyribonucleotide.

Authors:  H Dougan; J B Hobbs; J I Weitz; D M Lyster
Journal:  Nucleic Acids Res       Date:  1997-07-15       Impact factor: 16.971

2.  Mild detritylation of nucleic acid hydroxyl groups by warming up.

Authors:  Jozef Salon; Bo Zhang; Zhen Huang
Journal:  Nucleosides Nucleotides Nucleic Acids       Date:  2011-04       Impact factor: 1.381

3.  Highly efficient synthesis of oligodeoxyribonucleotides using alpha-phenyl cinnamoyl group for selective amino protection.

Authors:  A K Nagaich; K Misra
Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

Review 4.  Emerging and evolving concepts in gene essentiality.

Authors:  Giulia Rancati; Jason Moffat; Athanasios Typas; Norman Pavelka
Journal:  Nat Rev Genet       Date:  2017-10-16       Impact factor: 53.242

5.  Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.

Authors:  B A Connolly; P Rider
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

6.  Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

Authors:  V A Efimov; A A Buryakova; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

7.  Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.

Authors:  G A Bitter; K K Chen; A R Banks; P H Lai
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

8.  Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.

Authors:  T Tanaka; S Tamatsukuri; M Ikehara
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

9.  Synthesis and properties of oligodeoxynucleotides with an AP site at a preselected position.

Authors:  G R Stuart; R W Chambers
Journal:  Nucleic Acids Res       Date:  1987-09-25       Impact factor: 16.971

10.  Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

Authors:  B C Froehler; P G Ng; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.