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Literature DB >> 3299268

Synthesis and reactivity of intermediates formed in the T4 RNA ligase reaction.

Abstract

The intermediate adenylated donor derivatives A(5')pp(5')dTp and A(5')pp(5')GpGpGp have been prepared from suitable phosphorylating reagents activated by 1-hydroxybenzotriazole. Phosphodiester bond formation between donor and acceptor oligonucleotides as catalyzed by T4 RNA ligase is shown to be more efficient when the adenylated form of the donor molecule is used.

Entities: Chemical Species

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Year: 1987 PMID： 3299268 PMCID： PMC305962 DOI： 10.1093/nar/15.13.5289

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

1. Equimolar addition of oligoribonucleotides with T4 RNA ligase.

Authors: O C Uhlenbeck; V Cameron
Journal: Nucleic Acids Res Date: 1977-01 Impact factor: 16.971

2. Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase.

Authors: T E England; R I Gumport; O C Uhlenbeck
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

3. The effect of acceptor oligoribonucleotide sequence on the T4 RNA ligase reaction.

Authors: E Romaniuk; L W McLaughlin; T Neilson; P J Romaniuk
Journal: Eur J Biochem Date: 1982-07

4. Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.

Authors: T E England; O C Uhlenbeck
Journal: Biochemistry Date: 1978-05-30 Impact factor: 3.162

5. Reactions at the termini of tRNA with T4 RNA ligase.

Authors: A G Bruce; O C Uhlenbeck
Journal: Nucleic Acids Res Date: 1978-10 Impact factor: 16.971

6. Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.

Authors: A Sugino; T J Snoper; N R Cozzarelli
Journal: J Biol Chem Date: 1977-03-10 Impact factor: 5.157

7. Donor activation in the T4 RNA ligase reaction.

Authors: L W McLaughlin; N Piel; E Graeser
Journal: Biochemistry Date: 1985-01-15 Impact factor: 3.162

8. Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.

Authors: E Ohtsuka; T Miyake; K Nagao; H Uemura; S Nishikawa; M Sugiura; M Ikehara
Journal: Nucleic Acids Res Date: 1980-02-11 Impact factor: 16.971

9. A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

Authors: G A van der Marel; C A van Boeckel; G Wille; J H van Boom
Journal: Nucleic Acids Res Date: 1982-04-10 Impact factor: 16.971

10. Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters.

Authors: C B Reese; L Zard
Journal: Nucleic Acids Res Date: 1981-09-25 Impact factor: 16.971

3 in total

1. Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Authors: R Iwase; M Maeda; T Fujiwara; M Sekine; T Hata; K Miura
Journal: Nucleic Acids Res Date: 1992-04-11 Impact factor: 16.971

2. A novel type of defective viral genome suggests a unique strategy to establish and maintain persistent lymphocytic choriomeningitis virus infections.

Authors: B J Meyer; P J Southern
Journal: J Virol Date: 1997-09 Impact factor: 5.103

3. Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes.

Authors: Kyle Cole; Vivi Truong; Dale Barone; Glenn McGall
Journal: Nucleic Acids Res Date: 2004-06-17 Impact factor: 16.971

3 in total