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Literature DB >> 2582352

Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Abstract

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.

Entities: Chemical

Mesh：

Substances：

Year: 1985 PMID： 2582352 PMCID： PMC340973 DOI： 10.1093/nar/13.1.45

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

27 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Effect of salts and polyamines on T4 polynucleotide kinase.

Authors: J R Lillehaug; K Kleppe
Journal: Biochemistry Date: 1975-03-25 Impact factor: 3.162

3. A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes.

Authors: J Manning; M Pellegrini; N Davidson
Journal: Biochemistry Date: 1977-04-05 Impact factor: 3.162

4. Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge.

Authors: A Sodja; N Davidson
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

5. Strand-specific attachment of avidin-spheres to double-stranded poliovirus RNA.

Authors: O C Richards; E Ehrenfeld; J Manning
Journal: Proc Natl Acad Sci U S A Date: 1979-02 Impact factor: 11.205

6. Insolubilized biotin for the purification of avidin.

Authors: E Bayer; M Wilchek
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

7. A new method of in situ hybridization.

Authors: J E Manning; N D Hershey; T R Broker; M Pellegrini; H K Mitchell; N Davidson
Journal: Chromosoma Date: 1975-11-24 Impact factor: 4.316

8. Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels.

Authors: T R Broker; L M Angerer; P H Yen; N D Hershey; N Davidson
Journal: Nucleic Acids Res Date: 1978-02 Impact factor: 16.971

9. Use of avidin-sepharose to isolate and idenify biotin polypeptides from crude extracts.

Authors: J A Swack; G L Zander; M F Utter
Journal: Anal Biochem Date: 1978-06-15 Impact factor: 3.365

10. Biotin-labeled oligonucleotides: enzymatic synthesis and use as hybridization probes.

Authors: A Murasugi; R B Wallace
Journal: DNA Date: 1984-06

30 in total

Review 1. In situ hybridization of messenger RNA sequences.

Authors: L I Larsson
Journal: Histochem J Date: 1989-08

2. Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups.

Authors: C MacKellar; D Graham; D W Will; S Burgess; T Brown
Journal: Nucleic Acids Res Date: 1992-07-11 Impact factor: 16.971

3. Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

Authors: Y Kinoshita; K Nishigaki; Y Husimi
Journal: Nucleic Acids Res Date: 1997-09-15 Impact factor: 16.971

4. A general method for the synthesis of 3'-sulfhydryl and phosphate group containing oligonucleotides.

Authors: K C Gupta; P Sharma; P Kumar; S Sathyanarayana
Journal: Nucleic Acids Res Date: 1991-06-11 Impact factor: 16.971

5. Preparation and characterization of biotinylated psoralen.

Authors: W A Saffran; J T Welsh; R M Knobler; F P Gasparro; C R Cantor; R L Edelson
Journal: Nucleic Acids Res Date: 1988-08-11 Impact factor: 16.971

6. The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.

Authors: B S Sproat; B Beijer; P Rider
Journal: Nucleic Acids Res Date: 1987-08-11 Impact factor: 16.971

7. Oligonucleotides, part 5+: synthesis and fluorescence studies of DNA oligomers d(AT)5 containing adenines covalently linked at C-8 with dansyl fluorophore.

Authors: D Singh; V Kumar; K N Ganesh
Journal: Nucleic Acids Res Date: 1990-06-11 Impact factor: 16.971