Literature DB >> 192745

Sequence analysis of synthetic oligonucleotides by high-performance liquid anion-exchange chromatography.

J H van Boom, J F de Rooy.   

Abstract

A simple procedure for the sequential anlysis of small oligonucleotides is reported. The method is based on the simultaneous identification and quantitation of monomers released by venom phosphodiesterase digestion of oligonucleotides using high-performance anion-exchange chromatography on Permaphase AAX at room temperature and by applying isocratic elution conditions. In this way, the correct sequence of five oligomers, e.g., r-ACCUCC, r-CUGUU, r-AGGA, d-ATTACC and d-GGTAAT, could easily be established unambiguously.

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Year:  1977        PMID： 192745     DOI： 10.1016/s0021-9673(00)80930-9

Source DB:  PubMed          Journal:  J Chromatogr


  6 in total

1.  Fixed archival tissue. Purify DNA and primers for good PCR yield!

Authors:  G Weirich; M A Hornauer; T Brüning; H Höfler; H Brauch
Journal:  Mol Biotechnol       Date:  1997-12       Impact factor: 2.695

2.  Proton NMR studies on the covalently linked RNA-DNA hybrid r(GCG)d(TATACGC). Assignment of proton resonances by application of the nuclear Overhauser effect.

Authors:  J R Mellema; C A Haasnoot; G A van der Marel; G Wille; C A van Boeckel; J H van Boom; C Altona
Journal:  Nucleic Acids Res       Date:  1983-08-25       Impact factor: 16.971

3.  Chemical synthesis of genes for human insulin.

Authors:  R Crea; A Kraszewski; T Hirose; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

4.  Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.

Authors:  J H van Boom; P M Burgers; G van der Marel; C H Verdegaal; G Wille
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

5.  A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.

Authors:  G A van der Marel; C A van Boeckel; G Wille; J H van Boom
Journal:  Nucleic Acids Res       Date:  1982-04-10       Impact factor: 16.971

6.  Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.

Authors:  K Miyoshi; T Miyake; T Hozumi; K Itakura
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
  6 in total

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