Literature DB >> 6672769

Mass spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments.

L Alder, A Rosenthal, D Cech.   

Abstract

A new and convenient pyrolysis mass spectrometric approach for monitoring solid phase phosphate triester synthesis of oligodeoxyribonucleotides in 5'-3' direction has been developed. The base-typical fragment ions produced by pyrolysis at 280 degrees C of the polymer-bound oligonucleotide triesters in the mass spectrometer permit the analytical monitoring of DNA chain growth, using simple mathematical operations. The base composition can be determined directly from the polymer. In addition, minor nucleosides can easily be detected.

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Year:  1983        PMID： 6672769      PMCID： PMC326593          DOI： 10.1093/nar/11.23.8431

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Automated synthesis of gene fragments.

Authors:  G Alvarado-Urbina; G M Sathe; W C Liu; M F Gillen; P D Duck; R Bender; K K Ogilvie
Journal:  Science       Date:  1981-10-16       Impact factor: 47.728

2.  Synthesis of oligodeoxyribonucleotides on silica gel support.

Authors:  F Chow; T Kempe; G Palm
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

3.  Syringe method for stepwise chemical synthesis of oligonucleotides.

Authors:  T Tanaka; R L Letsinger
Journal:  Nucleic Acids Res       Date:  1982-05-25       Impact factor: 16.971

4.  Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides.

Authors:  T P Patel; T A Millican; C C Bose; R C Titmas; G A Mock; M A Eaton
Journal:  Nucleic Acids Res       Date:  1982-09-25       Impact factor: 16.971

5.  New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

Authors:  V A Efimov; S V Reverdatto; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

6.  Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.

Authors:  V Kohli; A Balland; M Wintzerith; R Sauerwald; A Staub; J P Lecocq
Journal:  Nucleic Acids Res       Date:  1982-11-25       Impact factor: 16.971

7.  Color coded triarylmethyl protecting groups useful for deoxypolynucleotide synthesis.

Authors:  E F Fisher; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

8.  Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.

Authors:  M J Gait; H W Matthes; M Singh; B S Sproat; R C Titmas
Journal:  Nucleic Acids Res       Date:  1982-10-25       Impact factor: 16.971

9.  Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

Authors:  H Ito; Y Ike; S Ikuta; K Itakura
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

10.  Sequence analysis of oligodeoxyribonucleotides by mass spectrometry. 1. Dinucleoside monophosphates.

Authors:  J L Wiebers; J A Shapiro
Journal:  Biochemistry       Date:  1977-03-22       Impact factor: 3.162
  10 in total

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