Literature DB >> 6169150

Automated synthesis of gene fragments.

G Alvarado-Urbina, G M Sathe, W C Liu, M F Gillen, P D Duck, R Bender, K K Ogilvie.   

Abstract

The DNA/RNA Synthesizer provides a complete and automated procedure for the synthesis of DNA sequences. Each base unit is added in a 30-minute cycle, permitting a tetradecamer to be constructed in 6 1/2 hours. The complete procedure is described, including a practical procedure for isolation and purification of the desired DNA sequence.

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Year:  1981        PMID： 6169150     DOI： 10.1126/science.6169150

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  37 in total

1.  Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.

Authors:  R T Pon; S Yu
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme.

Authors:  C K Barlowe; D R Appling
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

3.  Detection of gyrA gene mutations associated with ciprofloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencing.

Authors:  J J Goswitz; K E Willard; C E Fasching; L R Peterson
Journal:  Antimicrob Agents Chemother       Date:  1992-05       Impact factor: 5.191

4.  Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains.

Authors:  A K Bhargava; J T Woitach; E A Davidson; V P Bhavanandan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

5.  Suppression of a frameshift mutation in the recE gene of Escherichia coli K-12 occurs by gene fusion.

Authors:  C C Chu; A Templin; A J Clark
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

6.  Rational de novo gene synthesis by rapid polymerase chain assembly (PCA) and expression of endothelial protein-C and thrombin receptor genes.

Authors:  Tarlan G Mamedov; Nisha V Padhye; Hendrik Viljoen; Anuradha Subramanian
Journal:  J Biotechnol       Date:  2007-08-10       Impact factor: 3.307

Review 7.  Biological properties of single chemical-DNA adducts: a twenty year perspective.

Authors:  James C Delaney; John M Essigmann
Journal:  Chem Res Toxicol       Date:  2007-12-12       Impact factor: 3.739

8.  Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

Authors:  R T Pon; M J Damha; K K Ogilvie
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

9.  Regulation of expression of genes for light-harvesting antenna proteins LH-I and LH-II; reaction center polypeptides RC-L, RC-M, and RC-H; and enzymes of bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus by light and oxygen.

Authors:  Y S Zhu; J E Hearst
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

10.  Promoters selected from random DNA sequences.

Authors:  M S Horwitz; L A Loeb
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205
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