Literature DB >> 6174942

Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

S Ohno, T Taniguchi.   

Abstract

A hybrid plasmid was constructed from pSV2-Ecogpt (Mulligan and Berg, 1981) and the cloned 1.8 kilobase chromosomal DNA segment which contains the human interferon (HIFN-beta 1) gene and its flanking sequences. Cultured mouse FM3A cells were transfected by the hybrid plasmid and several Ecogpt+ clones were selected. Southern blot analysis of the DNA from these mouse cell clones showed that two of them contained a few copies of the 1.8 kilobase human DNA. Synthesis of mRNA characteristic of the HIFN-beta 1 mRNA increased as measured by RNA blot analysis when those clones were treated with Newcastle disease virus or with poly(I):poly(C). In addition, the induced mouse cell clones gave rise to the synthesis of human IFN-beta 1. These results suggest the presence of a unique nucleotide sequence in the 1.8 kilobase DNA which is sufficient for the inducer-responsive expression of the human IFN-beta 1 gene.

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Year:  1982        PMID： 6174942      PMCID： PMC326214          DOI： 10.1093/nar/10.3.967

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  28 in total

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9.  Human fibroblast interferon gene lacks introns.

Authors:  R M Lawn; J Adelman; A E Franke; C M Houck; M Gross; R Najarian; D V Goeddel
Journal:  Nucleic Acids Res       Date:  1981-03-11       Impact factor: 16.971

10.  Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene.

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Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205
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  23 in total

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4.  Transcriptional control of the mouse alpha 2(I) collagen gene: functional deletion analysis of the promoter and evidence for cell-specific expression.

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5.  A human IFN-beta 1 gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA.

Authors:  U Nir; B Cohen; L Chen; M Revel
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6.  Differential expression of human interferon genes.

Authors:  J Hiscott; K Cantell; C Weissmann
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7.  Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.

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8.  Regulated expression of human interferon beta 1 gene after transduction into cultured mouse and rabbit cells.

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Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

9.  Inducible expression of amplified human beta interferon genes in CHO cells.

Authors:  F McCormick; M Trahey; M Innis; B Dieckmann; G Ringold
Journal:  Mol Cell Biol       Date:  1984-01       Impact factor: 4.272

10.  Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells.

Authors:  J Content; L De Wit; J Tavernier; W Fiers
Journal:  Nucleic Acids Res       Date:  1983-05-11       Impact factor: 16.971
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