Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A mouse globin gene promoter is functional in SV40.

Literature DB >> 6254665

A mouse globin gene promoter is functional in SV40.

Abstract

We have constructed two SV40 recombinants carrying a complete mouse alpha-globin gene with its presumptive promoter region. In one recombinant the globin gene can be transcribed either from its own promoter or from the adjacent viral late region promoter. In the other efficient globin expression should depend only upon the promoter carried by the chromosomal globin gene. We show that both viruses direct the synthesis of functional globin mRNA in infected monkey kidney cells and that this mRNA has a 5' terminus indistinguishable from that of authentic globin mRNA. These results suggest that the cloned globin gene contains a functional promoter that is accurately recognized in monkey kidney cells.

Entities: Species

Mesh：

Substances：

Year: 1980 PMID： 6254665 DOI： 10.1016/0092-8674(80)90433-x

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

Keyword Cloud
Cited

18 in total

1. Expression of bacterial beta-galactosidase in animal cells.

Authors: G An; K Hidaka; L Siminovitch
Journal: Mol Cell Biol Date: 1982-12 Impact factor: 4.272

2. Introduction and expression of a fetal human globin gene in mouse fibroblasts.

Authors: N Hsiung; R S Roginski; P Henthorn; O Smithies; R Kucherlapati; A I Skoultchi
Journal: Mol Cell Biol Date: 1982-04 Impact factor: 4.272

3. Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

Authors: S Ohno; T Taniguchi
Journal: Nucleic Acids Res Date: 1982-02-11 Impact factor: 16.971

4. Expression of two human growth hormone genes in monkey cells infected by simian virus 40 recombinants.

Authors: G N Pavlakis; N Hizuka; P Gorden; P Seeburg; D H Hamer
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

5. Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1.

Authors: C M Wei; M Gibson; P G Spear; E M Scolnick
Journal: J Virol Date: 1981-09 Impact factor: 5.103

10. Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.

Authors: P R Young; R W Scott; D H Hamer; S M Tilghman
Journal: Nucleic Acids Res Date: 1982-05-25 Impact factor: 16.971

A mouse globin gene promoter is functional in SV40.

1. Expression of bacterial beta-galactosidase in animal cells.

2. Introduction and expression of a fetal human globin gene in mouse fibroblasts.

3. Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

4. Expression of two human growth hormone genes in monkey cells infected by simian virus 40 recombinants.

5. Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1.

6. Definition of the ovalbumin gene promoter by transfer of an ovalglobin fusion gene into cultured cells.

7. RNA processing errors in patients with beta-thalassemia.

8. In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.

9. Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.

10. Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.