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Literature DB >> 6956899

Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.

K Zinn, P Mellon, M Ptashne, T Maniatis.

Abstract

Beta(fibroblast)-interferon mRNA and protein are induced by the synthetic double-stranded RNA poly(I) X poly(C) in cultured human fibroblasts. To study the mechanism of this induction, we have isolated a human beta-interferon gene and inserted it in a vector plasmid containing DNA of the bovine papilloma virus. After removal of bacterial plasmid sequences, the bovine papilloma virus-beta-interferon recombinant was used to morphologically transform mouse fibroblasts. Analysis of DNA from the transformed cell lines indicated that this recombinant is propagated as a stable multicopy extrachromosomal element. Human beta-interferon mRNA and protein are inducible by poly(I) X poly(C) in all of these cell lines, and the mRNA is indistinguishable from beta-interferon mRNA synthesized by induced human cells.

Entities: Disease Species

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Year: 1982 PMID： 6956899 PMCID： PMC346792 DOI： 10.1073/pnas.79.16.4897

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.

1. Transformation of mammalian cells with genes from procaryotes and eucaryotes.

2. Selective extraction of polyoma DNA from infected mouse cell cultures.

3. Production of high-titered interferon in cultures of human diploid cells.

4. Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.

5. Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.

6. Controlled transcription of a human alpha-interferon gene introduced into mouse L cells.

7. Virus-specific transcription in bovine papillomavirus-transformed mouse cells.

8. Leukocyte and fibroblast interferon genes are located on human chromosome 9.

9. Human fibroblast interferon gene lacks introns.

10. Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.

1. Hepatitis B virus suppresses expression of human beta-interferon.

2. Optimizing gene expression in BPV-transformed cells: effects of cell type on enhancer/promoter interaction.

3. Transient expression of the beta interferon promoter in human cells.

4. Transcriptional trans-activating function of hepatitis B virus.

Review 5. The bovine papillomavirus genome and its uses as a eukaryotic vector.

6. Plasmidal maintenance of composite DNA derived from polyoma related plasmid, L factor.

7. Differential expression of human interferon genes.

8. High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.

9. Inducible expression of amplified human beta interferon genes in CHO cells.

10. Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells.