Literature DB >> 6258799

Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones.

E Buetti, H Diggelmann.   

Abstract

We have cloned circular unintegrated mouse mammary tumor virus (MMTV) DNA from infected rat hepatoma cells in bacteriophage lambda. Seven independent clones containing MMTV DNA of homogeneous length of 9 kb (five) or 10 kb (two) were identified. The five 9 kb clones had identical restriction maps consistent with that of 9 kb unintegrated DNA; the other two were aberrant. MMTV DNA inserts were purified, ligated and used for cotransfection of Ltk- cells together with a plasmid containing the thymidine kinase gene of herpes simplex virus. All Tk+ cell clones acquired new MMTV sequences and those transfected with the 9 kb MMTV DNA synthesized normal viral RNA and proteins. Viral gene expression was increased by the addition of dexamethasone.

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Year:  1981        PMID： 6258799     DOI： 10.1016/0092-8674(81)90129-x

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  53 in total

1.  Mutations in the hormone regulatory element of mouse mammary tumor virus differentially affect the response to progestins, androgens, and glucocorticoids.

Authors:  P L Gowland; E Buetti
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

2.  Molecular cloning and primary structure analysis of the mouse mammary tumor virus-related element from dwarf hamster genome.

Authors:  A T Tikhonenko; N S Vassetzky; T V Golovkina; A V Gudkov
Journal:  Virus Genes       Date:  1990-02       Impact factor: 2.332

3.  Regulation of gene amplification and expression in cells that constitutively express a temperature sensitive SV40 T-antigen.

Authors:  A Portela; S de la Luna; J A Melero; J Vara; A Jiménez; J Ortín
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971

4.  Molecular cloning and long terminal repeat sequences of human endogenous retrovirus genes related to types A and B retrovirus genes.

Authors:  M Ono
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

5.  Complete nucleotide sequence of a milk-transmitted mouse mammary tumor virus: two frameshift suppression events are required for translation of gag and pol.

Authors:  R Moore; M Dixon; R Smith; G Peters; C Dickson
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

6.  Induction of human beta-interferon synthesis with poly(rI . rC) in mouse cells transfected with cloned cDNA plasmids.

Authors:  P M Pitha; D M Ciufo; M Kellum; N B Raj; G R Reyes; G S Hayward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

7.  Polypeptide hormone regulation of gene transcription: specific 5' genomic sequences are required for epidermal growth factor and phorbol ester regulation of prolactin gene expression.

Authors:  S C Supowit; E Potter; R M Evans; M G Rosenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

8.  Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice.

Authors:  D E Graham; D Medina; G H Smith
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

9.  Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression in simian virus 40-transformed mink cells.

Authors:  D Owen; H Diggelmann
Journal:  J Virol       Date:  1983-01       Impact factor: 5.103

10.  Purified glucocorticoid receptor-hormone complex from rat liver cytosol binds specifically to cloned mouse mammary tumor virus long terminal repeats in vitro.

Authors:  M V Govindan; E Spiess; J Majors
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205
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